LOCUS       V035109                14830 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035109
VERSION     V035109
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..9732
                     /label="Fn1(NM_010233)"
                     /note="Fn1(NM_010233)"
                     /gene="Fn1"
     CDS             9739..9756
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     promoter        9800..10011
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10024..10292
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             10319..10918
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10925..11513
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11587..11820
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11892..12013
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12032..12167
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12205..12221)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12229..12245
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12253..12283)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12298..12319
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12607..13195)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13366..14226)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14227..14331)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14805..14821
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgctcagg ggtccgggac ccgggcggct gctgctgctg
     2341 gcagtcctgt gcctggggac ctcggtgcgc tgcaccgaag ccgggaagag caagaggcag
     2401 gctcagcaaa tcgtgcagcc tcaatccccg gtggctgtca gtcagagcaa gcctggctgt
     2461 tttgacaatg ggaagcacta tcagataaat cagcagtggg aacggaccta cctaggcaac
     2521 gccctggttt gtacctgcta tggaggaagc cggggtttta actgcgagag caagcctgag
     2581 cctgaagaga cttgctttga caaatacact gggaacactt acaaagtggg tgacacttat
     2641 gagcgcccta aagattccat gatctgggac tgtacctgca tcggggctgg gagaggcagg
     2701 atcagctgta ccattgcaaa tcgctgccat gaagggggtc agtcctacaa gattggcgac
     2761 aagtggagga ggccacatga gactggtggc tacatgttag agtgtctgtg tctgggaaat
     2821 ggaaaagggg aatggacctg caaacctata gctgagaagt gttttgatca tgctgctggg
     2881 acgtcctacg tcgtggggga gacctgggaa aagccctacc aaggctggat gatggtggac
     2941 tgtacttgtc taggcgaagg caatggacgc atcacctgta cctccagaaa cagatgcaac
     3001 gatcaggaca cccggacatc ctataggatt ggagacacgt ggagcaagaa ggacaaccga
     3061 ggaaacctgc ttcagtgtgt ctgcacaggc aatggcagag gggagtggaa gtgtgagcga
     3121 catgctctac aaagtgcttc agccggatct ggctccttca ctgatgtccg aacagctatt
     3181 taccaaccgc agactcaccc ccagcccgct ccctacggcc actgtgtcac cgacagtggt
     3241 gtggtctact ctgtgggaat gcagtggctg aagtcgcaag gaaacaagca aatgctgtgc
     3301 acgtgcctgg gcaatggcgt cagctgccag gagacagccg tgacccagac ttatggtggc
     3361 aattcaaacg gggagccctg tgtcctcccg ttcacctaca acggtaggac cttctattcc
     3421 tgcaccaccg aagggcggca agacggacat ctgtggtgta gcacaacttc caattacgaa
     3481 caagaccaga agtattcctt ctgcacagac catgcggttt tggttcagac tcgaggcgga
     3541 aattccaatg gtgctctgtg ccacttcccc ttcctgtaca acaaccggaa ttacaccgac
     3601 tgtacttctg agggtcgcag ggacaacatg aaatggtgcg gcaccaccca gaactacgat
     3661 gccgatcaga agtttggatt ctgcccaatg gctgcccacg aggagatctg cacaaccaat
     3721 gaaggggtca tgtatcgcat tggggatcag tgggataagc agcatgacct gggccacatg
     3781 atgaggtgca cgtgtgtggg gaacggtcgt ggagaatggg cctgcatccc ctactcccag
     3841 ctccgagacc agtgcatcgt tgatgacatt acttacaatg tgaacgacac gttccacaag
     3901 cgtcacgagg agggacatat gctgaactgt acctgctttg gtcagggccg gggcagatgg
     3961 aagtgtgacc ccattgacca gtgccaagat tcagagaccc ggacatttta ccagattggt
     4021 gactcctggg agaagtttgt gcatggtgtc cgataccagt gttactgcta cggccgtggc
     4081 atcggggagt ggcactgtca acctctgcag acctacccag gcacaactgg acctgtccaa
     4141 gtaattatca cggagacccc cagccagccc aattcccacc ccatccagtg gaatgccccg
     4201 gagccttcac acatcaccaa gtacattctc agatggagac ctaaaacctc tacgggtcgc
     4261 tggaaggaag ctaccattcc tggccacctt aactcctata ccatcaaagg cctgacccca
     4321 ggtgtgatct atgagggaca gctcatcagc atccagcagt atggccacag agaagtgaca
     4381 cgcttcgact tcaccaccag cgccagcacc cctgtgacca gcaacacggt gaccggagag
     4441 actgcgccct actctcctgt tgtggccact tctgaatctg taactgaaat cacagccagc
     4501 agctttgtgg tctcatgggt ctcagcctcc gacaccgtgt caggcttccg ggtggagtat
     4561 gagctgagcg aggagggaga tgaaccacag taccttgatc tcccaagcac ggccacttcc
     4621 gtgaacattc ctgacctgct cccgggcaga aagtacattg tcaatgtcta tcagatatct
     4681 gaggagggaa aacagagctt gatcctgtct acctcacaga ctacagcacc tgacgctcct
     4741 ccagacccta ccgtggacca ggttgatgat acttccattg ttgttcggtg gagtagaccc
     4801 caggcaccta tcacagggta tagaattgtc tattcacctt cagtagaagg cagtagcaca
     4861 gagctcaacc tccctgaaac ggccaactcc gtcaccctca gcgacctgca gcccggtgtt
     4921 cagtacaaca tcactatcta tgctgtggag gagaaccagg agagcacacc cgttttcatc
     4981 caacaagaga ccactggcac cccaagatct gataacgtcc cccctccgac ggacctacag
     5041 tttgtggaac tgactgatgt gaaagtcacc atcatgtgga cccctcctga tagtgtggtg
     5101 tctggatacc gtgtggaggt cctgcctgtc agcctgcccg gggaacatgg gcagaggctg
     5161 cctgtcaaca gaaatacctt tgctgaaatc actgggctgt cccctggggt cacgtacctc
     5221 ttcaaagtct ttgctgtgca ccagggcagg gaaagcaatc ctctgacggc acaacagacc
     5281 accaaactcg acgctcccac taacctccag tttgtcaatg aaactgacag aacagttctg
     5341 gtaacgtgga ctccacctcg agcccgtata gcaggctacc gactgaccgc gggcctgacc
     5401 cgaggaggcc agcccaagca gtacaatgtg ggacccttgg cctccaagta tcccctgaga
     5461 aatctgcagc ctgggtctga gtacaccgtg accttggtgg ctgtgaaagg gaaccagcag
     5521 agtcccaaag ccaccggagt ctttactacc ctgcagcctc tgcgctccat tccaccttac
     5581 aacaccgagg tgacagagac cacaattgtg atcacctgga cccccgctcc aaggattggc
     5641 ttcaagctgg gtgtacgacc gagccaggga ggtgaggcac cccgagaagt gacttcagac
     5701 tctgggagca ttgttgtgtc tggcttgact ccaggcgtgg aatacactta caccatccaa
     5761 gtcctgcgag atggccagga gagagatgca ccgattgtca acagagtagt gacaccgctg
     5821 tctccaccga ccaacttgca tctggaggca aaccctgaca ctggagtgct tactgtctcc
     5881 tgggagagga gcactacccc agatatcact ggctacagaa taactactac ccccacgaac
     5941 gggcagcagg ggacctctct ggaagaagtg gtccatgctg atcagagttc ctgcactttt
     6001 gagaacctga atcctggcct ggagtacaac gtcagtgttt acactgtcaa agatgacaag
     6061 gaaagtgccc ctatctctga taccgttgtc ccagaggtgc cccagctcac tgacctaagc
     6121 tttgttgata taactgattc aagcatcggc ctgaggtgga ccccgctaaa ctcttccacc
     6181 attatcgggt accgaatcac agtagttgcg gcaggagaag ggatccctat ttttgaagat
     6241 tttgtggact cctcagtagg atactacaca gttacagggc tggagcctgg cattgactat
     6301 gacatcagcg ttatcactct cattaatggc ggagagagtg cccctactac actgacacag
     6361 caaacggctg tccctcctcc cacggatctg cgattcacca atatcggtcc agacacgatg
     6421 cgggtcactt gggccccgcc tccgtccatc gagctgacca acctcttggt gcgctactca
     6481 cccgtgaaga atgaagagga cgttgcagag ctatccattt caccttcaga caatgccgtg
     6541 gtcctaacaa atctcctgcc tgggacagaa tacttagtca gtgtctccag tgtctacgaa
     6601 caacatgaga gcatccctct ccggggaagg cagaaaacag gtctcgattc cccaactggt
     6661 tttgattctt ctgatatcac cgccaactca ttcactgtcc actgggtggc tcctcgggcc
     6721 cccatcaccg gctacatcat ccgccatcac gccgagcatt ctgtcggaag acccaggcag
     6781 gatcgagtgc cgccctcgcg gaattccatc accctcacca accttaatcc gggcaccgag
     6841 tacgttgtca gcatcattgc tgttaatggc agagaggaga gcccgccact gattggccag
     6901 caagccacag tttctgatat tccgagagat ctggaggtca ttgcctccac ccccaccagc
     6961 ctgctcatca gttgggaacc ccctgccgtc tctgtgcgct attacagaat cacctacgga
     7021 gagacaggag gaaatagccc tgtccaggag ttcactgtgc ccggaagcaa gtccacagcc
     7081 accatcaaca acattaaacc aggagcagac tacaccatca ccctgtatgc tgtcactggc
     7141 cgtggggaca gtccagcaag cagcaagcca gtttccatca attataaaac agaaattgac
     7201 aagccgtccc agatgcaggt gacagatgtc caggacaaca gcatcagtgt caggtggctg
     7261 ccttcaactt ctcctgtgac aggctacaga gtgaccacca ctcccaaaaa tggcctagga
     7321 ccatcaaaaa ctaaaactgc cagtccagat caaacagaaa tgaccattga aggtttgcaa
     7381 cccactgtgg agtacgtggt tagtgtttat gctcagaacc ggaacggaga aagccagccc
     7441 ctggttcaaa ctgcagtgac caacattgat cgccctaaag gactggcatt cactgatgtg
     7501 gatgtcgatt ccatcaaaat tgcttgggaa agcccacagg ggcaagtttc caggtacagg
     7561 gtgacctact cgagccctga ggatggaatc cgggagcttt tccctgcacc tgatggtgaa
     7621 gacgacactg cagagctgca gggcctcagg ccggggtctg agtacacagt cagtgtggtt
     7681 gccttgcacg atgatatgga gagccagccc ctgattggaa tccagtccac agccattcct
     7741 gcgcccacca atctgaagtt cagtcaggtg acacccacca gctttactgc ccagtggata
     7801 gcacccagtg ttcagctcac tggctaccgg gtgcgggtga acccgaaaga gaagacagga
     7861 ccaatgaaag aaatcaacct ttctccagac agctcatcgg tgattgtgtc aggactcatg
     7921 gtggccacta aatacgaagt cagtgtctat gctctcaagg acacactgac aagcagacca
     7981 gcccagggag tcatcactac tctggagaat gttagccctc caagaagggc ccgtgtgacg
     8041 gacgctacag agaccaccat cactattagc tggagaacaa agacagagac aatcactggc
     8101 ttccaagtcg atgccatccc agccaatggc cagaccccag ttcagaggag catcagcccg
     8161 gatgttagaa gctacaccat tacaggttta cagccaggca ctgactacaa gatccacctg
     8221 tacactctga acgacaatgc ccggagctcg cccgtgatca tcgatgcctc caccgccatc
     8281 gacgcaccat ccaacctgcg gttcctgacc accacaccca actccttgct ggtgtcatgg
     8341 caggcgcccc gtgccaggat tactggctac attatcaagt atgagaagcc tggatcccct
     8401 cccagagaag tggtccctcg gccccgccct ggtgtcacgg aggccaccat tactggtctg
     8461 gagccaggaa ccgagtacac catctacgtc attgccctga agaacaatca gaagagtgag
     8521 cccctgattg ggaggaagaa gacagatgag cttccccaac tggttaccct tccacacccc
     8581 aatcttcatg gaccagagat cttggatgtt ccctccacag ttcaaaagac ccccttcatc
     8641 accaaccctg ggtatgacac cgaaaatggt attcagcttc ctggcacaac ccaccagcaa
     8701 cccagtgttg ggcaacaaat gatctttgag gaacatggct ttaggcggac aacgccaccc
     8761 actgcggcca cccccgtcag gcttaggcca agaccatacc tgccgaatgt agatgaggag
     8821 gtccaaatcg gtcatgttcc caggggagac gtagactacc acctctatcc tcatgttccg
     8881 ggcctcaatc caaatgcctc tacaggacag gaagctctct ctcagacaac catctcttgg
     8941 acgccgttcc aggagagttc tgagtacatc atttcatgcc aaccagttgg caccgacgaa
     9001 gagcccttac agttccaagt tcctggaact tctaccagtg cgactctgac tggccttacc
     9061 agaggggtca cctacaacat catagtggag gcactgcaga accagaggag gcacaaggtt
     9121 cgggaagagg ttgtgactgt gggcaacgct gtcagcgaag gcctgaacca gcctacagat
     9181 gactcatgct ttgaccctta cacggtttcc cattacgcca ttggagagga gtgggagcgg
     9241 ttgtctgacg ctggctttaa gctcacatgc cagtgcttgg gctttggcag tggtcatttc
     9301 agatgcgatt catctaaatg gtgccatgac aacggtgtca actacaagat cggagagaag
     9361 tgggatcggc agggagaaaa tggccagcgg atgagctgca catgcctcgg gaatggaaag
     9421 ggagaattca agtgtgatcc ccatgaagca acgtgctatg acgatgggaa gacctaccat
     9481 gtaggagaac agtggcagaa agaatatctc ggagccattt gctcctgcac gtgtttcgga
     9541 ggccagcggg gctggcgctg tgacaactgc cgtagacctg gggctgctga acccagtccc
     9601 gatggcacca ccggccacac ctacaaccag tatacacaga gatacaatca gagaacaaac
     9661 actaacgtaa attgccccat tgagtgcttc atgccgctag atgtgcaagc tgacagagac
     9721 gattctcgag agggatccca tcaccatcac catcactagc ggccgcgaag gatctgcgat
     9781 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     9841 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     9901 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     9961 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
    10021 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
    10081 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
    10141 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
    10201 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
    10261 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat
    10321 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
    10381 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
    10441 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    10501 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    10561 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    10621 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    10681 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    10741 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    10801 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    10861 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt
    10921 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
    10981 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
    11041 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
    11101 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
    11161 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
    11221 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
    11281 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
    11341 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
    11401 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
    11461 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac
    11521 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    11581 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg
    11641 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    11701 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    11761 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    11821 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    11881 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11941 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    12001 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc
    12061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    12121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag
    12181 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    12241 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    12301 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    12361 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    12421 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    12481 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    12541 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    12601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    12661 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    12721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12781 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12961 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    13021 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    13081 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    13141 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    13201 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    13261 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    13321 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    13381 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    13441 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    13501 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    13561 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    13621 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    13681 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    13741 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    13801 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    13861 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    13921 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13981 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    14041 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    14101 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    14161 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    14221 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    14281 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    14341 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    14401 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    14461 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    14521 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    14581 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    14641 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    14701 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    14761 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    14821 tgccaagctg
//
