LOCUS       V014627                10539 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014627
VERSION     V014627
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2302..3951
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             4027..5454
                     /label="tdTomato"
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     promoter        5509..5720
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             5733..6001
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6028..6624
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6634..7222
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7296..7529
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7601..7735
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7741..7876
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7962..7992)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8007..8028)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8316..8904)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9078..9935)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9936..10040)
                     /label="AmpR promoter"
     primer_bind     10514..10530
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca
     2341 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
     2401 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     2461 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     2521 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
     2581 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
     2641 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     2701 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     2761 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     2821 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     2881 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     2941 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     3001 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     3061 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     3121 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     3181 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     3241 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     3301 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     3361 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     3421 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     3481 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     3541 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     3601 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     3661 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     3721 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     3781 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     3841 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     3901 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gttagcggcc
     3961 gctgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggcccttcc
     4021 ggaaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     4081 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4141 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     4201 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     4261 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4321 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     4381 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     4441 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     4501 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     4561 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     4621 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     4681 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggtag cggcagctcc
     4741 ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag
     4801 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     4861 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     4921 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     4981 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     5041 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     5101 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     5161 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     5221 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     5281 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     5341 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     5401 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaagtc
     5461 gacggatccg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     5521 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     5581 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     5641 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     5701 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     5761 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     5821 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     5881 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     5941 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     6001 ccaagctgtg accggcgcct acgctagatg accgagtaca agcccacggt gcgcctcgcc
     6061 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6121 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6181 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6241 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6301 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6361 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6421 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6481 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6541 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6601 tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac ctctggatta caaaatttgt
     6661 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6721 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6781 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6841 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6901 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6961 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7021 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7081 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7141 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7201 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     7261 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7321 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7381 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7441 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7501 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7561 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7681 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7741 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7801 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7861 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
     7921 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7981 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8041 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8101 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8161 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8221 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8281 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8341 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8401 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8461 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8521 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8581 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8641 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8701 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8761 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8821 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8881 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8941 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9001 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9061 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9121 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9181 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9241 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9301 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9361 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9421 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9481 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9541 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9601 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9661 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9721 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9781 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9841 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9901 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9961 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10021 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    10081 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    10141 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    10201 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    10261 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10321 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    10381 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    10441 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    10501 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
