LOCUS       V029123                12810 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029123
VERSION     V029123
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5860
                     /label="ZMYND8(NM_183047)"
                     /note="ZMYND8(NM_183047)"
                     /gene="ZMYND8"
     CDS             5867..5932
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5982..6193
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6206..6474
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6503..6512
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6533..8182
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             8252..8305
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8306..8905
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8906..9493
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9567..9800
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9872..9993
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10012..10147
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10185..10201)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10209..10225
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10233..10263)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10278..10299
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10587..11175)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11346..12206)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12207..12311)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12785..12801
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgc atccacagag cttggctgaa gaggaaataa aaacagaaca
     2341 ggaggtggta gagggcatgg atatctctac tcgctccaaa gatcctggct ctgcagagag
     2401 aacagcccag aaaagaaagt tccccagccc tccacattct tccaatggcc actcgccgca
     2461 ggacacatca acaagcccca ttaaaaagaa aaagaaacct ggcttactga acagtaacaa
     2521 taaggagcag tcagaactaa gacatggtcc gttttactat atgaagcagc cactcaccac
     2581 agaccctgtt gatgttgtac cgcaggatgg acggaatgat ttctactgct gggtttgtca
     2641 ccgggaaggc caagtccttt gctgtgagct ctgtccccgg gtttatcacg ctaagtgtct
     2701 gagactgaca tcggaaccag agggggactg gttttgtcct gaatgtgaga aaattacagt
     2761 agcagaatgc atcgagaccc agagtaaagc catgacaatg ctcaccattg aacagttatc
     2821 ctacctgctc aagtttgcca ttcagaaaat gaaacagcca gggacagatg cattccagaa
     2881 gcccgttcca ttggaacagc accctgacta tgcggaatac atcttccatc caatggacct
     2941 ttgtacattg gaaaagaatg cgaaaaagaa aatgtatggc tgcacagaag ccttcctggc
     3001 tgatgcaaag tggattttgc acaactgcat catttataat gggggaaatc acaaattgac
     3061 gcaaatagcg aaagtagtca tcaaaatctg tgaacatgag atgaatgaaa tcgaagtatg
     3121 tccagaatgt tatctagctg cttgccaaaa acgagataac tggttttgtg agccttgtag
     3181 caatccacat cctttggtct gggccaaact gaaggggttt ccattctggc ctgcaaaagc
     3241 tctaagggat aaagacgggc aggtcgatgc ccgattcttt ggacaacatg acagggcctg
     3301 ggttccaata aataattgct acctcatgtc taaagaaatt cctttttctg tgaaaaagac
     3361 taagagcatc ttcaacagtg ccatgcaaga gatggaggtt tacgtggaga acatccgcag
     3421 gaagtttggg gtttttaatt actctccatt taggacaccc tacacaccca acagccagta
     3481 tcaaatgctg ctcgatccca ccaaccccag cgccggcact gccaagatag acaagcagga
     3541 gaaggtcaag ctcaactttg acatgacggc atcccccaag atcctgatga gcaagcctgt
     3601 gctgagtggg ggcacaggcc gccggatttc cttgtcggat atgccgcgct cccccatgag
     3661 cacaaactct tctgtgcaca cgggctccga cgtggagcag gatgctgaga agaaggccac
     3721 gtcgagccac ttcagtgcga gcgaggagtc catggacttc ctggataaga gcacagcttc
     3781 accagcctcc accaagacgg gacaagcagg gagtttatcc ggcagcccaa agcccttctc
     3841 tcctcaactg tcagctccta tcacgacgaa aacggacaaa acctccacca ccggcagcat
     3901 cctgaatctt aacctggatc gaagcaaagc tgagatggat ttgaaggagc tgagcgagtc
     3961 ggtccagcaa cagtccaccc ctgttcctct catctctccc aagcgccaga ttcgtagcag
     4021 gttccagctg aatcttgaca agaccataga gagttgcaaa gcacaattag gcataaatga
     4081 aatctcggaa gatgtctata cggccgtaga gcacagcgat tcggaggatt ctgagaagtc
     4141 agatagtagc gatagtgagt atatcagtga tgatgagcag aagtctaaga acgagccaga
     4201 agacacagag gacaaagaag gttgtcagat ggacaaagag ccatctgctg ttaaaaaaaa
     4261 gcccaagcct acaaacccag tggagattaa agaggagctg aaaagcacgt caccagccag
     4321 cgagaaggca gaccctggag cagtcaagga caaggccagc cctgagcctg agaaggactt
     4381 ttccgaaaag gcaaaacctt cacctcaccc cataaaggat aaactgaagg gaaaagatga
     4441 gacggattcc ccaacagtcc atttgggcct ggactctgat tcagagagcg aacttgtcat
     4501 agatttagga gaagaccatt ctgggcggga gggtcgaaaa aataagaagg aacccaaaga
     4561 accatctccc aaacaggatg ttgtaggtaa aactccacca tccacgacgg tgggcagcca
     4621 ttctcccccg gaaacaccgg tgctcacccg ctcttccgcc caaacttccg cggctggcgc
     4681 cacagccacc accagcacgt cctccacggt caccgtcacg gccccggccc ccgccgccac
     4741 aggaagccca gtgaaaaagc agaggccgct tttaccgaag gagactgccc cggccgtgca
     4801 gcgggtcgtg tggaactcat caactgtcca gcagaaggag atcacacaga gcccatccac
     4861 gtccaccatc accctggtga ccagcacaca gtcatcgccc ctggtcacca gctcggggtc
     4921 catgagcacc cttgtgtcct cagtcaacgc tgacctgccc atcgccactg cctcagctga
     4981 tgtcgccgct gatattgcca agtacactag caaaatgatg gatgcaataa aaggaacaat
     5041 gacagaaata tacaacgatc tttctaaaaa cactactgga agcacaatag ctgagattcg
     5101 caggctgagg atcgagatag agaagctcca gtggctgcac cagcaagagc tctccgaaat
     5161 gaaacacaac ttagagctga ccatggcgga gatgcggcag agcctggagc aggagcggga
     5221 ccggctcatc gccgaggtga agaagcagct ggagttggag aagcagcagg cggtggatga
     5281 gaccaagaag aagcagtggt gcgccaactg caagaaggag gccatctttt actgctgttg
     5341 gaacaccagc tactgtgact acccctgcca gcaagcccac tggcctgagc acatgaagtc
     5401 ctgcacccag tcagctactg ctcctcagca ggaagcggat gctgaggtga acacagaaac
     5461 actaaataag tcctcccagg ggagctcctc gagcacacaa tcagcacctt cagaaacggc
     5521 cagcgcctcc aaagagaagg agacgtcagc tgagaaaagc aaggagagtg gctcgaccct
     5581 tgacctttct ggctccagag agacgccctc ctccattctc ttaggctcca accaaggctc
     5641 tgttagcaaa aggtgtgaca agcaacctgc ctatgcccca accaccacag accaccagcc
     5701 gcaccccaac taccccgccc agaagtacca ttcccggagt aataaatcca gttggagcag
     5761 cagtgatgag aagaggggat cgacacgttc cgatcacaac accagtacca gcacgaagag
     5821 cctcctcccg aaagagtctc ggctggacac cttctgggac ctcgaggact acaaagacca
     5881 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaaggatc
     5941 cgcggccgca aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
     6001 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     6061 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     6121 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     6181 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     6241 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     6301 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     6361 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     6421 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     6481 gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc tgatggaaga
     6541 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
     6601 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     6661 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
     6721 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     6781 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     6841 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
     6901 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
     6961 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     7021 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
     7081 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     7141 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     7201 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     7261 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     7321 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     7381 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     7441 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     7501 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     7561 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     7621 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     7681 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     7741 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     7801 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     7861 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     7921 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     7981 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     8041 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     8101 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     8161 gaagggcgga aagatcgccg tgagatcccg cgctcagtcg tccaattctg ccgtggacgg
     8221 caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg
     8281 tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac
     8341 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8401 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     8461 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8521 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     8581 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8641 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8701 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8761 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     8821 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     8881 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt
     8941 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     9001 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     9061 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     9121 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     9181 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     9241 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     9301 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     9361 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     9421 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg
     9481 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     9541 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     9601 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9661 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9721 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9781 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9841 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     9901 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9961 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
    10021 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10081 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10141 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
    10201 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    10261 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    10321 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10381 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10441 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10501 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    11041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    11101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    11161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    11221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    11281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    11341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    11401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    11461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11641 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11701 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11761 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11821 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11881 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11941 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    12001 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    12061 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    12121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    12181 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    12241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    12301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    12361 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    12421 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    12481 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    12541 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    12601 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    12661 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    12721 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    12781 cgttgtaaaa cgacggccag tgccaagctg
//
