LOCUS       V020315                13839 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020315
VERSION     V020315
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..6889
                     /label="TOP2A(NM_001067)"
                     /note="TOP2A(NM_001067)"
                     /gene="TOP2A"
     CDS             6896..6961
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7011..7222
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7235..7503
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7532..7541
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7562..9211
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             9281..9334
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9335..9934
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9935..10522
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10596..10829
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10901..11022
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11041..11176
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11214..11230)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11238..11254
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11262..11292)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11307..11328
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11616..12204)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12375..13235)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13236..13340)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13814..13830
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg aagtgtcacc attgcagcct gtaaatgaaa atatgcaagt
     2341 caacaaaata aagaaaaatg aagatgctaa gaaaagactg tctgttgaaa gaatctatca
     2401 aaagaaaaca caattggaac atattttgct ccgcccagac acctacattg gttctgtgga
     2461 attagtgacc cagcaaatgt gggtttacga tgaagatgtt ggcattaact atagggaagt
     2521 cacttttgtt cctggtttgt acaaaatctt tgatgagatt ctagttaatg ctgcggacaa
     2581 caaacaaagg gacccaaaaa tgtcttgtat tagagtcaca attgatccgg aaaacaattt
     2641 aattagtata tggaataatg gaaaaggtat tcctgttgtt gaacacaaag ttgaaaagat
     2701 gtatgtccca gctctcatat ttggacagct cctaacttct agtaactatg atgatgatga
     2761 aaagaaagtg acaggtggtc gaaatggcta tggagccaaa ttgtgtaaca tattcagtac
     2821 caaatttact gtggaaacag ccagtagaga atacaagaaa atgttcaaac agacatggat
     2881 ggataatatg ggaagagctg gtgagatgga actcaagccc ttcaatggag aagattatac
     2941 atgtatcacc tttcagcctg atttgtctaa gtttaaaatg caaagcctgg acaaagatat
     3001 tgttgcacta atggtcagaa gagcatatga tattgctgga tccaccaaag atgtcaaagt
     3061 ctttcttaat ggaaataaac tgccagtaaa aggatttcgt agttatgtgg acatgtattt
     3121 gaaggacaag ttggatgaaa ctggtaactc cttgaaagta atacatgaac aagtaaacca
     3181 caggtgggaa gtgtgtttaa ctatgagtga aaaaggcttt cagcaaatta gctttgtcaa
     3241 cagcattgct acatccaagg gtggcagaca tgttgattat gtagctgatc agattgtgac
     3301 taaacttgtt gatgttgtga agaagaagaa caagggtggt gttgcagtaa aagcacatca
     3361 ggtgaaaaat cacatgtgga tttttgtaaa tgccttaatt gaaaacccaa cctttgactc
     3421 tcagacaaaa gaaaacatga ctttacaacc caagagcttt ggatcaacat gccaattgag
     3481 tgaaaaattt atcaaagctg ccattggctg tggtattgta gaaagcatac taaactgggt
     3541 gaagtttaag gcccaagtcc agttaaacaa gaagtgttca gctgtaaaac ataatagaat
     3601 caagggaatt cccaaactcg atgatgccaa tgatgcaggg ggccgaaact ccactgagtg
     3661 tacgcttatc ctgactgagg gagattcagc caaaactttg gctgtttcag gccttggtgt
     3721 ggttgggaga gacaaatatg gggttttccc tcttagagga aaaatactca atgttcgaga
     3781 agcttctcat aagcagatca tggaaaatgc tgagattaac aatatcatca agattgtggg
     3841 tcttcagtac aagaaaaact atgaagatga agattcattg aagacgcttc gttatgggaa
     3901 gataatgatt atgacagatc aggaccaaga tggttcccac atcaaaggct tgctgattaa
     3961 ttttatccat cacaactggc cctctcttct gcgacatcgt tttctggagg aatttatcac
     4021 tcccattgta aaggtatcta aaaacaagca agaaatggca ttttacagcc ttcctgaatt
     4081 tgaagagtgg aagagttcta ctccaaatca taaaaaatgg aaagtcaaat attacaaagg
     4141 tttgggcacc agcacatcaa aggaagctaa agaatacttt gcagatatga aaagacatcg
     4201 tatccagttc aaatattctg gtcctgaaga tgatgctgct atcagcctgg cctttagcaa
     4261 aaaacagata gatgatcgaa aggaatggtt aactaatttc atggaggata gaagacaacg
     4321 aaagttactt gggcttcctg aggattactt gtatggacaa actaccacat atctgacata
     4381 taatgacttc atcaacaagg aacttatctt gttctcaaat tctgataacg agagatctat
     4441 cccttctatg gtggatggtt tgaaaccagg tcagagaaag gttttgttta cttgcttcaa
     4501 acggaatgac aagcgagaag taaaggttgc ccaattagct ggatcagtgg ctgaaatgtc
     4561 ttcttatcat catggtgaga tgtcactaat gatgaccatt atcaatttgg ctcagaattt
     4621 tgtgggtagc aataatctaa acctcttgca gcccattggt cagtttggta ccaggctaca
     4681 tggtggcaag gattctgcta gtccacgata catctttaca atgctcagct ctttggctcg
     4741 attgttattt ccaccaaaag atgatcacac gttgaagttt ttatatgatg acaaccagcg
     4801 tgttgagcct gaatggtaca ttcctattat tcccatggtg ctgataaatg gtgctgaagg
     4861 aatcggtact gggtggtcct gcaaaatccc caactttgat gtgcgtgaaa ttgtaaataa
     4921 catcaggcgt ttgatggatg gagaagaacc tttgccaatg cttccaagtt acaagaactt
     4981 caagggtact attgaagaac tggctccaaa tcaatatgtg attagtggtg aagtagctat
     5041 tcttaattct acaaccattg aaatctcaga gcttcccgtc agaacatgga cccagacata
     5101 caaagaacaa gttctagaac ccatgttgaa tggcaccgag aagacacctc ctctcataac
     5161 agactatagg gaataccata cagataccac tgtgaaattt gttgtgaaga tgactgaaga
     5221 aaaactggca gaggcagaga gagttggact acacaaagtc ttcaaactcc aaactagtct
     5281 cacatgcaac tctatggtgc tttttgacca cgtaggctgt ttaaagaaat atgacacggt
     5341 gttggatatt ctaagagact tttttgaact cagacttaaa tattatggat taagaaaaga
     5401 atggctccta ggaatgcttg gtgctgaatc tgctaaactg aataatcagg ctcgctttat
     5461 cttagagaaa atagatggca aaataatcat tgaaaataag cctaagaaag aattaattaa
     5521 agttctgatt cagaggggat atgattcgga tcctgtgaag gcctggaaag aagcccagca
     5581 aaaggttcca gatgaagaag aaaatgaaga gagtgacaac gaaaaggaaa ctgaaaagag
     5641 tgactccgta acagattctg gaccaacctt caactatctt cttgatatgc ccctttggta
     5701 tttaaccaag gaaaagaaag atgaactctg caggctaaga aatgaaaaag aacaagagct
     5761 ggacacatta aaaagaaaga gtccatcaga tttgtggaaa gaagacttgg ctacatttat
     5821 tgaagaattg gaggctgttg aagccaagga aaaacaagat gaacaagtcg gacttcctgg
     5881 gaaagggggg aaggccaagg ggaaaaaaac acaaatggct gaagttttgc cttctccgcg
     5941 tggtcaaaga gtcattccac gaataaccat agaaatgaaa gcagaggcag aaaagaaaaa
     6001 taaaaagaaa attaagaatg aaaatactga aggaagccct caagaagatg gtgtggaact
     6061 agaaggccta aaacaaagat tagaaaagaa acagaaaaga gaaccaggta caaagacaaa
     6121 gaaacaaact acattggcat ttaagccaat caaaaaagga aagaagagaa atccctggtc
     6181 tgattcagaa tcagatagga gcagtgacga aagtaatttt gatgtccctc cacgagaaac
     6241 agagccacgg agagcagcaa caaaaacaaa attcacaatg gatttggatt cagatgaaga
     6301 tttctcagat tttgatgaaa aaactgatga tgaagatttt gtcccatcag atgctagtcc
     6361 acctaagacc aaaacttccc caaaacttag taacaaagaa ctgaaaccac agaaaagtgt
     6421 cgtgtcagac cttgaagctg atgatgttaa gggcagtgta ccactgtctt caagccctcc
     6481 tgctacacat ttcccagatg aaactgaaat tacaaaccca gttcctaaaa agaatgtgac
     6541 agtgaagaag acagcagcaa aaagtcagtc ttccacctcc actaccggtg ccaaaaaaag
     6601 ggctgcccca aaaggaacta aaagggatcc agctttgaat tctggtgtct ctcaaaagcc
     6661 tgatcctgcc aaaaccaaga atcgccgcaa aaggaagcca tccacttctg atgattctga
     6721 ctctaatttt gagaaaattg tttcgaaagc agtcacaagc aagaaatcca agggggagag
     6781 tgatgacttc catatggact ttgactcagc tgtggctcct cgggcaaaat ctgtacgggc
     6841 aaagaaacct ataaagtacc tggaagagtc agatgaagat gatctgtttc tcgaggacta
     6901 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     6961 gtaaggatcc gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     7021 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     7081 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     7141 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     7201 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     7261 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     7321 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     7381 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     7441 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     7501 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct
     7561 gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
     7621 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
     7681 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
     7741 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
     7801 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
     7861 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
     7921 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
     7981 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
     8041 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
     8101 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
     8161 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
     8221 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
     8281 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
     8341 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta
     8401 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct
     8461 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc
     8521 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata
     8581 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     8641 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     8701 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg
     8761 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     8821 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc
     8881 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca
     8941 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact
     9001 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     9061 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     9121 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     9181 aaaggccaag aagggcggaa agatcgccgt gagatcccgc gctcagtcgt ccaattctgc
     9241 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct
     9301 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     9361 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     9421 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     9481 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     9541 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     9601 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     9661 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     9721 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     9781 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9841 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9901 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     9961 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    10021 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    10081 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    10141 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    10201 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    10261 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    10321 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
    10381 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    10441 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
    10501 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
    10561 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
    10621 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
    10681 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10741 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10801 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
    10861 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
    10921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
    11041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    11101 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    11161 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
    11221 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    11281 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    11341 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    11401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    11461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    11521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    11581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    11641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    11701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    11761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    11821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    11881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    11941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    12001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    12061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    12121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    12181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    12241 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    12301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    12361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    12421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    12481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    12541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    12601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    12661 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    12721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    12781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    12841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    12901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12961 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    13021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    13081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    13141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    13201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    13261 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    13321 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    13381 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    13441 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    13501 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    13561 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    13621 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    13681 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    13741 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    13801 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
