LOCUS       V040101                13037 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040101
VERSION     V040101
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6159
                     /label="Smc4(NM_133786)"
                     /note="Smc4(NM_133786)"
                     /gene="Smc4"
     promoter        6209..6420
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6433..6701
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6730..6739
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6760..8409
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             8479..8532
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8533..9132
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9133..9720
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9794..10027
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10099..10220
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10239..10374
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10412..10428)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10436..10452
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10460..10490)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10505..10526
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10814..11402)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11573..12433)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12434..12538)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13012..13028
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcggcgt aagggcacca agccctccac tgcctgccac
     2341 caggaggaag ggccgccgcc ctcccaggac ggtgcccaca gcgacgaaga gatggagcaa
     2401 ccggccggcg aagcggagag cgctgcaccc gcaaagcctc caggtgagga gctggataac
     2461 agaagtttag aggagatttt gaatagtatc cctccacccc cacctccagc catggccagt
     2521 gaagctggag ctcctcgtct catgataact catattgtaa accaaaactt caagtcctat
     2581 gctggagaga aagttctggg acctttccat aagcgctttt cctgtattat tggaccaaat
     2641 ggcagtggaa aatccaatgt tattgattcc atgctttttg tgtttggcta tcgagcacaa
     2701 aaaataaggt ctaaaaagct atcggtactg atacataatt ctgatgaaca caaggatatt
     2761 cagagttgta ctgtagaagt tcattttcaa aagataattg ataaggaagg agatgattac
     2821 gaagtccttc ctaacagcaa cttctatgta tccagaaccg cctacagaga tagcacttct
     2881 gtctaccaca taagtgggaa gaagaagacg tttaaagatg ttggaaatct ccttagaagc
     2941 catggcattg acttagacca taatcgattt ttaatcttgc agggtgaagt tgaacagatt
     3001 gctatgatga aacccaaagg ccagactgaa catgatgaag gtatgcttga gtatttagaa
     3061 gatataattg gctgtggacg gctaaatgaa cctattaaag tcttgtgtcg gagagttgaa
     3121 atattaaatg aacacagagg agaaaagtta aatagagtga aaatggtaga aaaggagaag
     3181 gatgcactag agggagagaa aaacatagcc attgagttcc ttaccctgga aaatgaaatg
     3241 tttaagaaaa agaatcacat ctgtcaatac tacatttatg atctgcagaa tagaattgct
     3301 gaaataacga ctcaaaagga aaaaattcat gaagatacta aagaaattac tgagaagagc
     3361 aatgtgcttt ccaatgaaat gaaagcgaag aattcagctg taaaggatgt agaaaagaag
     3421 ctcaataaag ttacaaaatt tatcgaacag aataaagaaa aatttacaca actagatttg
     3481 gaagatgttc aagttaggga aaaattaaaa catgctacaa gtaaagccaa gaaactggag
     3541 aaacaacttc aaaaagataa agaaaaggtt gaagagctga aaagtgtacc tgccaagagt
     3601 aagactgtca taaatgagac aacaactaga aacaattcgc ttgagaaaga aagagagaaa
     3661 gaagaaaaaa aattaaaaga ggtcatggac agccttaagc aggaaacaca ggggcttcag
     3721 aaagaaaagg agattcaaga aaaggaactt atgggtttta acaaatcagt aaatgaagca
     3781 cgttcaaaga tggaagttgc tcagtcagaa cttgatatct atctcagtcg acataataca
     3841 gcagtgtctc aattaagtaa ggcgaaggaa gccttaatta cagcttctga gactctcaag
     3901 gagaggaaag ctgcaatcaa ggatataaac acaaagctcc ctcagacgca acaggaacta
     3961 aaggagaaag aaaaagaact tcagaaactg acacaagaag aaataaactt aaaaagtttg
     4021 gttcatgatc ttttccaaaa agttgaagaa gcaaagagtt ccttagcaat gaatcgaagt
     4081 agggggaaag tacttgatgc aataattcaa gaaaaaaaat caggcaggat cccaggaata
     4141 tatggaagat tgggagactt aggagctatt gatgaaaaat atgacattgc tatttcatcc
     4201 tgttgccatg cattggacta cattgttgtt gattctattg atacagccca agaatgtgta
     4261 aacttcctta aaaagcataa tattgggatt gcaactttca taggtttgga taagatgaca
     4321 gtgtgggcca aaaaaatgag caaaattcaa actcctgaaa atactcctcg gttatttgat
     4381 ctagttaaag tgaaaaatga ggaaatccgc caagcctttt actttgcttt acgagatacc
     4441 ttggtggcta acaatttgga tcaagctaca agagtagcgt accaaagaga cagacgctgg
     4501 agagtagtga ctctgcaggg acagatcata gaacagtcag gtacaatgag tggcggtgga
     4561 agcaaggtga tgagaggaag gatgggctct tcggttatcg atgaaatctc tgtagaagag
     4621 gtaaataaga tggaatccca gttggaaagg cattctaaac aagcaatgca aatccaagaa
     4681 cagaaagtac aacatgaaga agcagtagtt aagttacggc acagtgaacg agacatgaga
     4741 aatacactgg aaaagttcgc tgcaagcatc cagggcttat cagagcaaga agagtatctg
     4801 tgtgtacaaa ttaaagaact ggaagctaac gttcttacta cggcccctga cagaaaacaa
     4861 cagaaactgc tggaagaaaa tgtcagtgtt ttcaaaaaag aatatgatgc tgtggctgag
     4921 aaagctggta aagttgaagc tgagattaaa cggctacaca ataccatcat agatatcaac
     4981 aaccgaaaac tcaaggccca gcaaaacaaa cttgacacga taaataagca gcttgatgaa
     5041 tgtgcttctg ctattactaa ggcccaagta gcaatcaaga ctgctgacag aaatcttaaa
     5101 aaggcacagg attctgtttg ccgcacagag aaagaaataa aagacactga aaaagaaata
     5161 aatgacttaa agacagagct aaaaaatatt gaagacaagg cagaagaggt cataaacaat
     5221 acaaaaactg cagagacatc cttaccagaa atccagaaag aacatcgtaa tctacttcag
     5281 gaactaaaag ttattcaaga aaatgaacat gctcttcaga aagatgcact tagtattaag
     5341 ttgaaacttg aacagataga tggccacatt tctgaacata actcaaaaat aaaatactgg
     5401 caaaaagaga tttcaaaaat aaaattgcat cctgttgaag ataatcctgt tgagacagta
     5461 gcagttctaa gccaggagga gcttgaggca atcaagaatc cagagtctat aacaaatgaa
     5521 attgcacttt tggaagctca gtgtcgtgaa atgaaaccaa accttggagc catcgctgag
     5581 tataaaaaaa aggaagattt atatttgcaa agagtagccg aactggacaa aattacttct
     5641 gaaagagata attttagaca agcatatgaa gatcttcgaa aacaaaggct gaatgaattt
     5701 atggctggtt tttacgtaat aacaaataaa ctaaaagaaa actaccagat gctcacattg
     5761 ggaggagatg ctgaactgga gcttgtggac agtttagatc ctttttctga aggaatcatg
     5821 ttcagtgttc ggccacctaa gaaaagttgg aagaagatct ttaacctctc aggaggcgag
     5881 aaaaccctta gttccttggc cttagtgttt gctcttcatc actacaagcc cactcccctc
     5941 tacttcatgg atgagattga tgcagctctg gattttaaaa atgtgtctat tgttgcattt
     6001 tatatatatg agcaaacaaa aaatgcccag ttcataataa tctctcttcg aaataatatg
     6061 tttgagattt cagacagact tattggcatc tataaaacat ataatagtac aaaaagtgtt
     6121 gcagtgaacc caaaacaaat tgcgtctaaa ggactctgtt gaggatccgc ggccgcaagg
     6181 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     6241 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     6301 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     6361 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     6421 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     6481 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     6541 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6601 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6661 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6721 acgctagacg ccaccatgga gagcgacgag agcggcctga tggaagacgc caaaaacata
     6781 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
     6841 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
     6901 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
     6961 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     7021 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     7081 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     7141 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
     7201 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
     7261 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
     7321 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
     7381 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
     7441 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     7501 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     7561 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
     7621 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
     7681 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
     7741 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
     7801 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     7861 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
     7921 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
     7981 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     8041 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
     8101 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
     8161 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     8221 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     8281 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     8341 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     8401 atcgccgtga gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     8461 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     8521 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8581 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8641 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8701 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8761 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8821 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     8881 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     8941 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     9001 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     9061 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     9121 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     9181 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     9241 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     9301 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9361 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9421 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9481 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     9541 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9601 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9661 cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc
     9721 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     9781 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     9841 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9901 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9961 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10021 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
    10081 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10201 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
    10261 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10321 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
    10381 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10441 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10501 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10561 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11221 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    11281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12541 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    12601 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    12661 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    12721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    12781 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    12841 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    12901 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    12961 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    13021 cggccagtgc caagctg
//
