LOCUS       V033479                10686 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033479
VERSION     V033479
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3813
                     /label="SHMT2(NM_005412)"
                     /note="SHMT2(NM_005412)"
                     /gene="SHMT2"
     promoter        3858..4069
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4082..4350
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4379..4388
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4409..6058
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             6128..6181
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6182..6781
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6782..7369
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7443..7676
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7748..7869
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7888..8023
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8061..8077)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8085..8101
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8109..8139)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8154..8175
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8463..9051)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9222..10082)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10083..10187)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10661..10677
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgctgtac ttctctttgt tttgggcggc tcggcctctg
     2341 cagagatgtg ggcagctggt caggatggcc attcgggctc agcacagcaa cgcagcccag
     2401 actcagactg gggaagcaaa caggggctgg acaggccagg agagcctgtc ggacagtgat
     2461 cctgagatgt gggagttgct gcagagggag aaggacaggc agtgtcgtgg cctggagctc
     2521 attgcctcag agaacttctg cagccgagct gcgctggagg ccctggggtc ctgtctgaac
     2581 aacaagtact cggagggtta tcctggcaag agatactatg ggggagcaga ggtggtggat
     2641 gaaattgagc tgctgtgcca gcgccgggcc ttggaagcct ttgacctgga tcctgcacag
     2701 tggggagtca atgtccagcc ctactccggg tccccagcca acctggccgt ctacacagcc
     2761 cttctgcaac ctcacgaccg gatcatgggg ctggacctgc ccgatggggg ccatctcacc
     2821 cacggctaca tgtctgacgt caagcggata tcagccacgt ccatcttctt cgagtctatg
     2881 ccctataagc tcaaccccaa aactggcctc attgactaca accagctggc actgactgct
     2941 cgacttttcc ggccacggct catcatagct ggcaccagcg cctatgctcg cctcattgac
     3001 tacgcccgca tgagagaggt gtgtgatgaa gtcaaagcac acctgctggc agacatggcc
     3061 cacatcagtg gcctggtggc tgccaaggtg attccctcgc ctttcaagca cgcggacatc
     3121 gtcaccacca ctactcacaa gactcttcga ggggccaggt cagggctcat cttctaccgg
     3181 aaaggggtga aggctgtgga ccccaagact ggccgggaga tcccttacac atttgaggac
     3241 cgaatcaact ttgccgtgtt cccatccctg caggggggcc cccacaatca tgccattgct
     3301 gcagtagctg tggccctaaa gcaggcctgc acccccatgt tccgggagta ctccctgcag
     3361 gttctgaaga atgctcgggc catggcagat gccctgctag agcgaggcta ctcactggta
     3421 tcaggtggta ctgacaacca cctggtgctg gtggacctgc ggcccaaggg cctggatgga
     3481 gctcgggctg agcgggtgct agagcttgta tccatcactg ccaacaagaa cacctgtcct
     3541 ggagaccgaa gtgccatcac accgggcggc ctgcggcttg gggccccagc cttaacttct
     3601 cgacagttcc gtgaggatga cttccggaga gttgtggact ttatagatga aggggtcaac
     3661 attggcttag aggtgaagag caagactgcc aagctccagg atttcaaatc cttcctgctt
     3721 aaggactcag aaacaagtca gcgtctggcc aacctcaggc aacgggtgga gcagtttgcc
     3781 agggccttcc ccatgcctgg ttttgatgag cattgaggcg gccgcaagga tctgcgatcg
     3841 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3901 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3961 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     4021 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     4081 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     4141 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     4201 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     4261 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     4321 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc
     4381 caccatggag agcgacgaga gcggcctgat ggaagacgcc aaaaacataa agaaaggccc
     4441 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
     4501 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
     4561 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
     4621 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
     4681 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
     4741 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
     4801 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
     4861 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
     4921 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
     4981 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
     5041 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
     5101 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     5161 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     5221 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
     5281 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     5341 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
     5401 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
     5461 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     5521 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
     5581 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     5641 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     5701 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     5761 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     5821 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     5881 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     5941 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     6001 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgag
     6061 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     6121 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     6181 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6241 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6301 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6361 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6421 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6481 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6541 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6601 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6661 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6721 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6781 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6841 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6901 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6961 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7021 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7081 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7141 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7201 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7261 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7321 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt
     7381 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7441 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7681 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7741 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7801 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7861 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     7921 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7981 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     8041 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8101 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8161 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8221 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8281 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8341 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8401 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8461 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8521 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8581 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8641 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8701 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8761 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8821 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8881 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8941 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9001 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9061 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9121 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9181 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9241 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9301 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9361 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9421 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9481 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9541 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9601 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9661 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9721 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9781 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9841 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9901 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9961 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10021 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10081 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10141 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10201 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    10261 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    10321 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    10381 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    10441 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10501 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    10561 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    10621 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    10681 aagctg
//
