LOCUS       V036924                10970 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036924
VERSION     V036924
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4092
                     /label="Nfe2l2(NM_010902)"
                     /note="Nfe2l2(NM_010902)"
                     /gene="Nfe2l2"
     promoter        4142..4353
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4366..4634
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4663..4672
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4693..6342
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             6412..6465
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6466..7065
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7066..7653
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7727..7960
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8032..8153
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8172..8307
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8345..8361)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8369..8385
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8393..8423)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8438..8459
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8747..9335)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9506..10366)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10367..10471)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10945..10961
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatggac ttggagttgc caccgccagg actacagtcc
     2341 cagcaggaca tggatttgat tgacatcctt tggaggcaag acatagatct tggagtaagt
     2401 cgagaagtgt ttgactttag tcagcgacag aaggactatg agctggaaaa acagaaaaaa
     2461 ctcgaaaagg aaagacaaga gcaactccag aaggaacagg agaaggcctt ttttgctcag
     2521 tttcaactgg atgaagaaac aggagaattc ctcccaattc agccggccca gcacatccag
     2581 acagacacca gtggatccgc cagctactcc caggttgccc acattcccaa acaagatgcc
     2641 ttgtactttg aagactgtat gcagcttttg gcagagacat tcccatttgt agatgaccat
     2701 gagtcgcttg ccctggatat ccccagccac gctgaaagtt cagtcttcac tgcccctcat
     2761 caggcccagt ccctcaatag ctctctggag gcagccatga ctgatttaag cagcatagag
     2821 caggacatgg agcaagtttg gcaggagcta ttttccattc ccgaattaca gtgtcttaat
     2881 accgaaaaca agcagctggc tgatactacc gctgttccca gcccagaagc cacactgaca
     2941 gaaatggaca gcaattacca tttttactca tcgatctcct cgctggaaaa agaagtgggc
     3001 aactgtggtc cacatttcct tcatggtttt gaggattctt tcagcagcat cctctccact
     3061 gatgatgcca gccagctgac ctccttagac tcaaatccca ccttaaacac agattttggc
     3121 gatgaatttt attctgcttt catagcagag cccagtgacg gtggcagcat gccttcctcc
     3181 gctgccatca gtcagtcact ctctgaactc ctggacggga ctattgaagg ctgtgacctg
     3241 tcactgtgta aagctttcaa cccgaagcac gctgaaggca caatggaatt caatgactct
     3301 gactctggca tttcactgaa cacgagtccc agccgagcgt ccccagagca ctccgtggag
     3361 tcttccattt acggagaccc accgcctggg ttcagtgact cggaaatgga ggagctagat
     3421 agtgcccctg gaagtgtcaa acagaacggc cctaaagcac agccagcaca ttctcctgga
     3481 gacacagtac agcctctgtc accagctcaa gggcacagtg ctcctatgcg tgaatcccaa
     3541 tgtgaaaata caacaaaaaa agaagttccc gtgagtcctg gtcatcaaaa agccccattc
     3601 acaaaagaca aacattcaag ccgcttagag gctcatctca cacgagatga gcttagggca
     3661 aaagctctcc atattccatt ccctgtcgaa aaaatcatta acctccctgt tgatgacttc
     3721 aatgaaatga tgtccaagga gcaattcaat gaagctcagc tcgcattgat ccgagatata
     3781 cgcaggagag gtaagaataa agtcgccgcc cagaactgta ggaaaaggaa gctggagaac
     3841 attgtcgagc tggagcaaga cttgggccac ttaaaagacg agagagaaaa actactcaga
     3901 gaaaagggag aaaacgacag aaacctccat ctactgaaaa ggcggctcag caccttgtat
     3961 cttgaagtct tcagcatgtt acgtgatgag gatggaaagc cttactctcc cagtgaatac
     4021 tctctgcagc aaaccagaga tggcaatgtg ttccttgttc ccaaaagcaa gaagccagat
     4081 acaaagaaaa actagggatc cgcggccgca aggatctgcg atcgctccgg tgcccgtcag
     4141 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4201 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4261 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4321 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     4381 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     4441 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     4501 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     4561 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     4621 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     4681 gagagcggcc tgatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg
     4741 ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     4801 cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac
     4861 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     4921 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     4981 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     5041 agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt
     5101 ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg
     5161 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     5221 aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg
     5281 aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc
     5341 gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     5401 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     5461 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc
     5521 cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc
     5581 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc
     5641 gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc
     5701 aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     5761 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     5821 gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg
     5881 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     5941 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac
     6001 cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc
     6061 atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac
     6121 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     6181 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     6241 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     6301 gagatcctca taaaggccaa gaagggcgga aagatcgccg tgagatcccg cgctcagtcg
     6361 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6421 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6481 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6541 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6601 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6661 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6721 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6781 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6841 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6901 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6961 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7021 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7081 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7141 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7201 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7261 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7321 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7381 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7441 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7501 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7561 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag
     7621 acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     7681 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7741 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7801 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7861 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7921 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7981 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     8041 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8101 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8161 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8221 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8281 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     8341 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8401 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8461 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8521 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8581 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8641 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8701 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8941 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9181 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9661 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9721 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9781 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9841 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9901 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9961 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10021 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10081 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10141 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10201 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10261 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10321 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10381 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10441 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10501 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    10561 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    10621 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    10681 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    10741 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10801 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10861 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10921 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
