LOCUS       V028013                10230 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028013
VERSION     V028013
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..3280
                     /label="Gimap4(NM_174990)"
                     /note="Gimap4(NM_174990)"
                     /gene="Gimap4"
     CDS             3287..3352
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        3402..3613
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             3626..3894
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      3923..3932
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3953..5602
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             5672..5725
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5726..6325
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6326..6913
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6987..7220
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7292..7413
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7432..7567
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7605..7621)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7629..7645
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7653..7683)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7698..7719
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8007..8595)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8766..9626)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9627..9731)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10205..10221
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg aagtccagtg cggtggtgcg gggttcatcc cagaaagttc
     2341 aaggagcagc catgagcttg gaaaccaaga tcaaggaatt ccccaactga gaattgtctt
     2401 acttggaaaa actggagcag gaaagagttc aacagggaac agtatccttg gggaaaaagt
     2461 gtttaattct ggcatttgtg caaaatccat caccaaggtc tgtgaaaaaa gggtgagcac
     2521 ctgggatggg aaagagcttg ttgtcgtgga tacacctggt atttttgaca ctgaggtacc
     2581 agatgctgac acacaaaggg agatcactcg ctatgttgcc ctgacctctc cagggcctca
     2641 tgctctgctc ctggtagttc cactggggcg ttatactgtg gaagaacaca aggctacaca
     2701 gaaaattctg gacatgtttg gaaaacaggc tagaagattc atgattctct tgctcaccag
     2761 gaaggatgac ttagaagaca ctgatatcca tgagtactta gagaaggctc ctaaattctt
     2821 tcaagaggtg atgcatgagt tccagaatcg ctactgtttg ttcaacaaca gagcctcagg
     2881 cgctgaaaag gaagagcaga agatgcaatt gttgaccttg gtccagagca tggtgaggga
     2941 aaatggtgga agatgcttta ctaataagat gtatgaaagt gccgagtgtg tgatccagaa
     3001 agagaccttg aggatgcaag aattatacag agaggagcta gagagggaga aagcacggat
     3061 aagacgggag tatgaagaac agattaaaga tctgagagat gaactggaga gggaaataag
     3121 aagggcacgt atggagaggg aattcaagga gagggaggct atctttacaa agaatcaaca
     3181 aaatgctagg aaggaagttg agaacacaag tatgatactt gaattaatta taaaagcatg
     3241 ggagattgct tcctttatct tcaaccagtt tatgaaagac ctcgaggact acaaagacca
     3301 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaaggatc
     3361 cgcggccgca aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3421 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     3481 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3541 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3601 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     3661 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     3721 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     3781 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     3841 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     3901 gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc tgatggaaga
     3961 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
     4021 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     4081 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
     4141 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     4201 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     4261 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
     4321 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
     4381 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     4441 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
     4501 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     4561 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     4621 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     4681 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     4741 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     4801 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     4861 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     4921 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     4981 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     5041 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     5101 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     5161 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     5221 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     5281 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     5341 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     5401 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     5461 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     5521 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     5581 gaagggcgga aagatcgccg tgagatcccg cgctcagtcg tccaattctg ccgtggacgg
     5641 caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg
     5701 tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac
     5761 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5821 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5881 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5941 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     6001 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     6061 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     6121 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     6181 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     6241 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     6301 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt
     6361 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6421 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6481 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6541 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6601 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6661 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6721 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6781 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6841 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg
     6901 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6961 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     7021 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7081 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7141 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7201 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7261 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7321 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7381 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7441 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7501 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7561 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
     7621 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7681 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7741 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7801 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9001 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9061 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9121 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9181 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9241 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9301 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9361 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9421 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9481 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9541 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9601 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9661 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9721 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9781 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9841 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9901 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9961 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    10021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    10081 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10141 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10201 cgttgtaaaa cgacggccag tgccaagctg
//
