LOCUS       V032594                12409 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032594
VERSION     V032594
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EYFP"
     CDS             3049..5514
                     /label="FGFR1(NM_023110)"
                     /note="FGFR1(NM_023110)"
                     /gene="FGFR1"
     CDS             5521..5538
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     promoter        5581..5792
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5805..6073
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6102..6111
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6132..7781
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             7851..7904
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7905..8504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8505..9092
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9166..9399
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9471..9592
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9611..9746
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9784..9800)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9808..9824
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9832..9862)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9877..9898
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10186..10774)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10945..11805)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11806..11910)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12384..12400
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca gtgcttcgcc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg cggtggagga tcaggtggag gcggatccat gtggagctgg
     3061 aagtgcctcc tcttctgggc tgtgctggtc acagccacac tctgcaccgc taggccgtcc
     3121 ccgaccttgc ctgaacaagc ccagccctgg ggagcccctg tggaagtgga gtccttcctg
     3181 gtccaccccg gtgacctgct gcagcttcgc tgtcggctgc gggacgatgt gcagagcatc
     3241 aactggctgc gggacggggt gcagctggcg gaaagcaacc gcacccgcat cacaggggag
     3301 gaggtggagg tgcaggactc cgtgcccgca gactccggcc tctatgcttg cgtaaccagc
     3361 agcccctcgg gcagtgacac cacctacttc tccgtcaatg tttcagatgc tctcccctcc
     3421 tcggaggatg atgatgatga tgatgactcc tcttcagagg agaaagaaac agataacacc
     3481 aaaccaaacc gtatgcccgt agctccatat tggacatccc cagaaaagat ggaaaagaaa
     3541 ttgcatgcag tgccggctgc caagacagtg aagttcaaat gcccttccag tgggacccca
     3601 aaccccacac tgcgctggtt gaaaaatggc aaagaattca aacctgacca cagaattgga
     3661 ggctacaagg tccgttatgc cacctggagc atcataatgg actctgtggt gccctctgac
     3721 aagggcaact acacctgcat tgtggagaat gagtacggca gcatcaacca cacataccag
     3781 ctggatgtcg tggagcggtc ccctcaccgg cccatcctgc aagcagggtt gcccgccaac
     3841 aaaacagtgg ccctgggtag caacgtggag ttcatgtgta aggtgtacag tgacccgcag
     3901 ccgcacatcc agtggctaaa gcacatcgag gtgaatggga gcaagattgg cccagacaac
     3961 ctgccttatg tccagatctt gaagactgct ggagttaata ccaccgacaa agagatggag
     4021 gtgcttcact taagaaatgt ctcctttgag gacgcagggg agtatacgtg cttggcgggt
     4081 aactctatcg gactctccca tcactctgca tggttgaccg ttctggaagc cctggaagag
     4141 aggccggcag tgatgacctc gcccctgtac ctggagatca tcatctattg cacaggggcc
     4201 ttcctcatct cctgcatggt ggggtcggtc atcgtctaca agatgaagag tggtaccaag
     4261 aagagtgact tccacagcca gatggctgtg cacaagctgg ccaagagcat ccctctgcgc
     4321 agacaggtaa cagtgtctgc tgactccagt gcatccatga actctggggt tcttctggtt
     4381 cggccatcac ggctctcctc cagtgggact cccatgctag caggggtctc tgagtatgag
     4441 cttcccgaag accctcgctg ggagctgcct cgggacagac tggtcttagg caaacccctg
     4501 ggagagggct gctttgggca ggtggtgttg gcagaggcta tcgggctgga caaggacaaa
     4561 cccaaccgtg tgaccaaagt ggctgtgaag atgttgaagt cggacgcaac agagaaagac
     4621 ttgtcagacc tgatctcaga aatggagatg atgaagatga tcgggaagca taagaatatc
     4681 atcaacctgc tgggggcctg cacgcaggat ggtcccttgt atgtcatcgt ggagtatgcc
     4741 tccaagggca acctgcggga gtacctgcag gcccggaggc ccccagggct ggaatactgc
     4801 tacaacccca gccacaaccc agaggagcag ctctcctcca aggacctggt gtcctgcgcc
     4861 taccaggtgg cccgaggcat ggagtatctg gcctccaaga agtgcataca ccgagacctg
     4921 gcagccagga atgtcctggt gacagaggac aatgtgatga agatagcaga ctttggcctc
     4981 gcacgggaca ttcaccacat cgactactat aaaaagacaa ccaacggccg actgcctgtg
     5041 aagtggatgg cacccgaggc attatttgac cggatctaca cccaccagag tgatgtgtgg
     5101 tctttcgggg tgctcctgtg ggagatcttc actctgggcg gctccccata ccccggtgtg
     5161 cctgtggagg aacttttcaa gctgctgaag gagggtcacc gcatggacaa gcccagtaac
     5221 tgcaccaacg agctgtacat gatgatgcgg gactgctggc atgcagtgcc ctcacagaga
     5281 cccaccttca agcagctggt ggaagacctg gaccgcatcg tggccttgac ctccaaccag
     5341 gagtacctgg acctgtccat gcccctggac cagtactccc ccagctttcc cgacacccgg
     5401 agctctacgt gctcctcagg ggaggattcc gtcttctctc atgagccgct gcccgaggag
     5461 ccctgcctgc cccgacaccc agcccagctt gccaatggcg gactcaaacg ccgcctcgag
     5521 caccatcacc atcaccatta gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt
     5581 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     5641 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     5701 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     5761 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     5821 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     5881 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     5941 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     6001 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     6061 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg
     6121 agagcggcct gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc
     6181 tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
     6241 ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact
     6301 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
     6361 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
     6421 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
     6481 gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt
     6541 tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg
     6601 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
     6661 atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga
     6721 actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg
     6781 tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
     6841 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
     6901 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc
     6961 ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca
     7021 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg
     7081 ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca
     7141 ggcaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
     7201 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
     7261 ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga
     7321 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
     7381 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc
     7441 gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca
     7501 tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg
     7561 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     7621 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
     7681 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
     7741 agatcctcat aaaggccaag aagggcggaa agatcgccgt gagatcccgc gctcagtcgt
     7801 ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag
     7861 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc
     7921 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     7981 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     8041 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8101 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8161 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8221 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8281 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8341 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8401 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8461 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt
     8521 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8581 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8641 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8701 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8761 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8821 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8881 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     8941 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     9001 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga
     9061 cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     9121 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     9181 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     9241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     9421 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     9481 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9541 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9601 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9661 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9721 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc
     9781 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9841 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9901 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9961 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10021 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10081 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10201 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10261 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10321 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10381 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10441 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10501 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10561 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10621 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10681 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10981 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11341 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    11941 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    12001 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    12061 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    12121 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    12181 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    12241 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    12301 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    12361 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
