LOCUS       V036092                11222 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036092
VERSION     V036092
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5316
                     /label="Flt3(NM_010229)"
                     /note="Flt3(NM_010229)"
                     /gene="Flt3"
     promoter        5381..5592
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5605..5873
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5902..5911
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5932..6594
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6664..6717
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6718..7317
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7318..7905
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7979..8212
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8284..8405
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8424..8559
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8597..8613)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8621..8637
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8645..8675)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8690..8711
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8999..9587)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9758..10618)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10619..10723)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11197..11213
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc gggcgttggc gcagcgcagc
     2341 gaccggcggc tgctgctgct tgttgttttg tcagtaatga ttcttgagac cgttacaaac
     2401 caagacctgc ctgtgatcaa gtgtgtttta atcagtcatg agaacaatgg ctcatcagcg
     2461 ggaaagccat catcgtaccg aatggtgcga ggatccccag aagacctcca gtgtgccccg
     2521 aggcgccaga gtgaagggac ggtatatgaa gcggccaccg tggaggtggc cgagtctggg
     2581 tccatcaccc tgcaagtgca gctcgccacc ccaggggacc tttcctgcct ctgggtcttt
     2641 aagcacagct ccctgggctg ccagccgcac tttgatttac aaaacagagg aatcgtttcc
     2701 atggccatct tgaacgtgac agagacccag gcaggagaat acctactcca tattcagagc
     2761 gaagccgcca actacacagt actgttcaca gtgaatgtaa gagatacaca gctgtacgtg
     2821 ctaagaagac cttactttag gaagatggaa aaccaggacg cactgctctg catctccgag
     2881 ggtgttccag agcccactgt ggagtgggtg ctctgcagct cccacaggga aagctgtaaa
     2941 gaagaaggcc ctgctgttgt cagaaaggag gaaaaggtac ttcatgagtt gttcggaaca
     3001 gacatcagat gctgtgctag aaatgcactg ggccgcgaat gcaccaagct gttcaccata
     3061 gatctaaacc aggctcctca gagcacactg ccccagttat tcctgaaagt gggggaaccc
     3121 ttgtggatca ggtgtaaggc catccatgtg aaccatggat tcgggctcac ctgggagctg
     3181 gaagacaaag ccctggagga gggcagctac tttgagatga gtacctactc cacaaacagg
     3241 accatgattc ggattctctt ggcctttgtg tcttccgtgg gaaggaacga caccggatat
     3301 tacacctgct cttcctcaaa gcaccccagc cagtcagcgt tggtgaccat cctagaaaaa
     3361 gggtttataa acgctaccag ctcgcaagaa gagtatgaaa ttgacccgta cgaaaagttc
     3421 tgcttctcag tcaggtttaa agcgtaccca cgaatccgat gcacgtggat cttctctcaa
     3481 gcctcatttc cttgtgaaca gagaggcctg gaggatgggt acagcatatc taaattttgc
     3541 gatcataaga acaagccagg agagtacata ttctatgcag aaaatgatga cgcccagttc
     3601 accaaaatgt tcacgctgaa tataagaaag aaacctcaag tgctagcaaa tgcctcagcc
     3661 agccaggcgt cctgttcctc tgatggctac ccgctaccct cttggacctg gaagaagtgt
     3721 tcggacaaat ctcccaattg cacggaggaa atcccagaag gagtttggaa taaaaaggct
     3781 aacagaaaag tgtttggcca gtgggtgtcg agcagtactc taaatatgag tgaggccggg
     3841 aaagggcttc tggtcaaatg ctgtgcgtac aattctatgg gcacgtcttg cgaaaccatc
     3901 tttttaaact caccaggccc cttccctttc atccaagaca acatctcctt ctatgcgacc
     3961 attgggctct gtctcccctt cattgttgtt ctcattgtgt tgatctgcca caaatacaaa
     4021 aagcaattta ggtacgagag tcagctgcag atgatccagg tgactggccc cctggataac
     4081 gagtacttct acgttgactt cagggactat gaatatgacc ttaagtggga gttcccgaga
     4141 gagaacttag agtttgggaa ggtcctgggg tctggcgctt tcgggagggt gatgaacgcc
     4201 acggcctatg gcattagtaa aacgggagtc tcaattcagg tggcggtgaa gatgctaaaa
     4261 gagaaagctg acagctgtga aaaagaagct ctcatgtcgg agctcaaaat gatgacccac
     4321 ctgggacacc atgacaacat cgtgaatctg ctgggggcat gcacactgtc agggccagtg
     4381 tacttgattt ttgaatattg ttgctatggt gacctcctca actacctaag aagtaaaaga
     4441 gagaagtttc acaggacatg gacagagatt tttaaggaac ataatttcag tttttaccct
     4501 actttccagg cacattcaaa ttccagcatg cctggttcac gagaagttca gttacacccg
     4561 cccttggatc agctctcagg gttcaatggg aatttaattc attctgaaga tgagattgaa
     4621 tatgaaaacc agaagaggct ggcagaagaa gaggaggaag atttgaacgt gctgacgttt
     4681 gaagacctcc tttgctttgc gtaccaagtg gccaaaggca tggaattcct ggagttcaag
     4741 tcgtgtgtcc acagagacct ggcagccagg aatgtgttgg tcacccacgg gaaggtggtg
     4801 aagatctgtg actttggact ggcccgagac atcctgagcg actccagcta cgtcgtcagg
     4861 ggcaacgcac ggctgccggt gaagtggatg gcacctgaga gcttatttga agggatctac
     4921 acaatcaaga gtgacgtctg gtcctacggc atccttctct gggagatatt ttcactgggt
     4981 gtgaaccctt accctggcat tcctgtcgac gctaacttct ataaactgat tcagagtgga
     5041 tttaaaatgg agcagccatt ctatgccaca gaagggatat actttgtaat gcaatcctgc
     5101 tgggcttttg actcaaggaa gcggccatcc ttccccaacc tgacttcatt tttaggatgt
     5161 cagctggcag aggcagaaga agcgatgtat cagaacatgg gtggcaacgt cccagaacat
     5221 ccatccatct accaaaacag gcggcccctc agcagagagg caggctcaga gccgccatcg
     5281 ccacaggccc aggtgaagat tcacggagaa agaagttagg gatccctgcg atatccagcg
     5341 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5401 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5461 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5521 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5581 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5641 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5701 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5761 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5821 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5881 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     5941 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     6001 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     6061 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     6121 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     6181 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     6241 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     6301 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     6361 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     6421 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     6481 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     6541 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     6601 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     6661 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     6721 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6781 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6841 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6901 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6961 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7021 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7081 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7141 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7201 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7261 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     7321 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7381 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7441 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7501 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7561 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7621 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7681 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7741 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7801 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc
     7861 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga
     7921 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7981 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc
     8041 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8101 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8161 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8221 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8281 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8341 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8401 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     8461 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8521 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
     8581 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8641 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8701 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8821 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8881 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8941 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9001 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9061 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9421 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9661 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9721 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10681 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10741 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10801 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10861 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10921 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    10981 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    11041 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    11101 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    11161 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    11221 tg
//
