LOCUS       V036942                13972 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036942
VERSION     V036942
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7998
                     /label="FNDC1(NM_032532)"
                     /note="FNDC1(NM_032532)"
                     /gene="FNDC1"
     CDS             8005..8070
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8114..8325
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8338..8606
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8635..8644
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8665..9327
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9397..9450
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9451..10050
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10051..10639
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10713..10946
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11018..11139
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11179..11314
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11347..11363)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11371..11387
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11395..11425)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11440..11461
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11749..12337)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12508..13368)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13369..13473)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13947..13963
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cccccgaggc cggggcgacc
     2341 ctgcgcgcgc cgcgccggct gtcctgggcg gcgctgctgc tcttggccgc gctgctcccc
     2401 gtcgcctcct cggcggcggc ctcagttgac cacccactga agccaaggca tgtgaaactg
     2461 ctgtccacta aaatgggcct gaaagtcacg tgggacccac ccaaagatgc taccagtaga
     2521 cctgtggagc attacaacat tgcctatggg aagtcactga aaagtcttaa atacatcaag
     2581 gtgaatgcgg agacatactc cttccttatt gaggatgtgg agccgggggt agtgtacttt
     2641 gtgctgctta ctgcagaaaa ccacagtgga gtgagccgtc ctgtttacag agctgaaagc
     2701 ccacctggag gtgaatggat cgagattgat ggttttccca ttaagggtcc aggaccattt
     2761 aatgaaaccg tcacagaaaa ggaagtgccc aacaagccct tgcgtgtgcg tgtccggtcc
     2821 tcagatgaca ggctgtccgt tgcgtggaag gcaccacgcc tgtctggagc caagagtcca
     2881 cgcagatcac ggggttttct cctgggctac ggggagagtg gccggaagat gaattatgtt
     2941 ccactgacaa gagatgaacg gacacacgaa attaaaaagc tagcctcgga atccgtgtat
     3001 gtggtctccc tgcagtccat gaactctcag ggccggagcc aaccagtcta cagggctgcc
     3061 ctaacaaagc gaaagatttc agaagaggac gaattggatg tacctgacga catcagcgtc
     3121 cgggttatgt catctcagtc tgtgcttgtg tcctgggtgg atcctgttct ggaaaaacag
     3181 aagaaagttg ttgcatcaag acagtacacc gtgcgctatc gagagaaggg ggaattggcc
     3241 aggtgggatt ataagcagat cgctaacagg cgtgtgctga ttgagaacct gattccagac
     3301 actgtgtatg aatttgcagt ccgtatttca cagggtgaaa gagatggcaa atggagtacg
     3361 tcagtcttcc aaagaacacc agaatctgcc cctaccacag ctcctgaaaa cttgaacgtc
     3421 tggccagtca atggcaaacc tacagttgtc gctgcatctt gggatgcgct accagagact
     3481 gaggggaaag tgaaagaata cattctttca tacgccccgg ctctcaaacc atttggagca
     3541 aagtccctca cctatcctgg agacactact tctgccctgg tggatggtct gcagcctggg
     3601 gaacgctatc ttttcaaaat ccgggccaca aacaggagag gcctgggacc tcactccaaa
     3661 gccttcattg tcgctatgcc aacaaccagt aaggcggatg ttgagcagaa cacggaggac
     3721 aatgggaaac ccgaaaaacc tgagccttcc tcaccttctc ccagagctcc agcttcctcc
     3781 caacacccct ctgtgcctgc ttctccccaa gggagaaatg ccaaggacct tcttcttgac
     3841 ttgaagaaca aaatattggc taatggtggg gcgccccgaa aaccccagct tcgcgccaag
     3901 aaggcagagg agctggatct tcagtcgaca gaaatcactg gggaggagga gctgggttcc
     3961 cgggaggact cgcccatgtc accctcagac acccaagacc agaaacggac cctgaggccg
     4021 ccaagtagac acggccactc ggtggttgct cccggcagga ctgcagtgag ggcccggatg
     4081 ccagcgctgc cccgaaggga aggcgtagat aagcctggct tttccctggc cacgcagccc
     4141 cgcccagggg cgcccccctc ggcttcggcc tctcctgccc accacgcgtc cacccagggc
     4201 acctctcatc gtccttccct gcctgccagc ttgaatgaca acgacttggt ggactcagac
     4261 gaagatgagc gcgctgtggg ctccctccac cccaagggcg ccttcgccca gccccggcca
     4321 gccctgtccc ccagccgcca gtccccgtcc agcgttctcc gcgacagaag ctctgtgcac
     4381 cccggcgcaa agccagcctc gccggcccgg aggacccccc attcaggggc cgcagaggaa
     4441 gattccagtg cctcagcccc accctcaaga ctttctccac cccatggggg atcatctcgg
     4501 ctgctgccca cccagccaca cctgagctct ccactttcca agggcgggaa ggatggtgag
     4561 gacgccccag ccaccaactc caatgcgcca tcacggtcca ccatgtcctc ctccgtctct
     4621 tctcatctct cgtccaggac gcaggtctct gagggagcgg aggcttctga tggtgaaagc
     4681 cacggtgacg gcgataggga agacggcgga aggcaggcgg aggccacggc ccagacgctg
     4741 cgggcccggc ctgcctctgg acacttccat ttgctcagac acaaaccctt tgctgccaac
     4801 gggaggtctc caagcaggtt cagcattggg cggggacctc ggctgcagcc ctccagctcc
     4861 ccacagtcga ctgtgccctc ccgagcccac cccagggttc cctctcactc tgattcccac
     4921 cctaagctta gctcaggtat ccatggagac gaggaggatg agaagccgct tcctgccacc
     4981 gttgtcaatg accacgtgcc ttcctcctcc aggcagccca tctcccgggg ctgggaggac
     5041 ttaaggagaa gcccgcagag aggggccagc ctgcatcgga aggaacccat cccagagaac
     5101 cccaaatcca caggggcaga tacacatcct cagggcaagt actcctccct ggcctccaag
     5161 gctcaggatg ttcaacagag cacagacgcg gacacggagg gtcattctcc caaagcacag
     5221 ccagggtcca cagaccgcca cgcgtcccct gctcgtccgc ccgcagcacg gtcacagcag
     5281 catcccagtg ttcccagaag gatgacaccc ggccgggccc cacaacagca gccccctcct
     5341 cccgtcgcca cgtcccagca ccacccggga ccccagagca gagacgcggg tcggtcacct
     5401 tcccagccca ggctctcact gacccaggcc gggcggcccc gccccacgtc gcagggccgc
     5461 tcccactcct cctcggaccc ttacacggcg agctccagag ggatgctccc cacggccctc
     5521 cagaaccagg acgaggatgc ccagggcagc tacgacgacg acagcacaga agtcgaggcc
     5581 caggatgtgc gggcccccgc gcacgccgcg cgcgccaagg aggcagctgc gtcccttccc
     5641 aagcaccagc aggtggagtc tcccacaggc gcaggggcag gtggcgacca caggtcccag
     5701 cgcggacatg cggcctcccc cgccaggccc agccgacccg gcggccccca gtcccgcgcc
     5761 cgggtaccca gcagggcagc gccggggaag tcggagcctc cttccaagcg gcccctgtcc
     5821 tccaagtccc agcagtcggt ctcagccgag gacgacgagg aggaggacgc gggatttttt
     5881 aaaggcggga aagaagacct tctgtcttcc tctgtgccaa agtggccctc ttcctccact
     5941 cccaggggcg gcaaagacgc cgatgggagc ctcgccaagg aagagaggga gcctgccatc
     6001 gcgcttgccc ctcgcggagg gagcctggct cctgtgaagc gacctctccc cccacctcca
     6061 ggcagctccc ccagggcctc ccacgtccct tcccgactgc cgcctcgcag cgctgccacc
     6121 gtgagccccg tcgcgggcac ccacccctgg ccgcagtaca ccacgcgcgc cccacctggc
     6181 cacttctcca ccaccccgat gctgtccttg cgccagagga tgatgcatgc cagattccgt
     6241 aaccctctct cccgacagcc tgccagaccc tcttacagac aaggttataa tggcagacca
     6301 aatgtagaag ggaaagtcct tcctggtagt aatggaaaac cgaatggaca gagaattatc
     6361 aatggccctc aaggaacaaa gtgggttgtg gaccttgatc gtgggttagt attgaatgca
     6421 gaaggaaggt acctccaaga ttcacatgga aatcctcttc ggattaaact aggaggagat
     6481 ggtcgaacca ttgtagatct ggaagggacc cccgtggtga gtcctgacgg cctcccactc
     6541 tttgggcagg ggcgacatgg cacacctctg gccaatgccc aagataagcc aattttgagt
     6601 cttggaggaa agccgctggt gggcttggag gtcatcaaaa aaaccaccca tccccctacc
     6661 actaccatgc agcccaccac tactacgacg cccctgccta ccactacaac cccgaggccc
     6721 accactgcca ccaccaggcg gacgaccaca accaggcgga caaccaccag acgaccgact
     6781 acgactgtga ggaccaccac acgcaccacc accacaacga cccccactcc aaccaccccg
     6841 ataccaacgt gtccccctgg gaccttggaa cggcacgacg atgatggcaa cctgataatg
     6901 agctccaatg ggatcccaga gtgctacgct gaagaagatg agttctcagg cttggagact
     6961 gacactgcag tacctacgga agaggcctac gttatatatg atgaagatta tgaatttgag
     7021 acgtcaaggc caccaaccac cactgagcct tcgaccactg ctaccacacc gagggtgatc
     7081 ccagaggaag gcgccatcag ttcctttcct gaagaagaat ttgatctggc tggaaggaaa
     7141 cgatttgttg ctccttacgt gacgtaccta aataaagacc catcagcccc gtgctctctg
     7201 actgatgcac tggatcactt ccaagtggac agcctggatg aaatcatccc caatgacctg
     7261 aagaagagtg acctgcctcc ccagcatgct ccccgcaaca tcaccgtggt ggccgtggaa
     7321 ggttgccact catttgtcat tgtggactgg gacaaagcca ccccaggaga tgtggtcaca
     7381 ggttacttgg tttacagtgc atcctatgaa gacttcatca ggaacaagtg gtccactcaa
     7441 gcttcatcag taactcactt gcccattgag aacctaaagc ccaacacgag gtattatttt
     7501 aaagtgcaag cacaaaatcc tcatggctac ggacctatca gcccttcggt ctcatttgtc
     7561 accgaatcag ataatcctct gcttgttgtg aggcccccag gcggtgagcc tatctggatc
     7621 ccattcgctt tcaaacatga tcccagctac acggactgcc atggacggca atatgtgaag
     7681 cgcacgtggt atcgaaagtt cgtgggagtt gttctttgta attcactgag gtataaaatc
     7741 tacctcagtg acaacctgaa agatacattc tacagcattg gagacagctg gggaagaggt
     7801 gaagaccatt gccaatttgt ggattcacac cttgatggaa gaacagggcc tcagtcctat
     7861 gtagaagccc tccctactat tcaaggctac tatcgccagt atcgtcagga gcctgtcagg
     7921 tttgggaaca tcggcttcgg aaccccctac tactatgtgg gctggtacga gtgtggggtc
     7981 tccatccctg gaaagtgggg atccgactac aaagaccatg acggtgatta taaagatcat
     8041 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     8101 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     8161 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     8221 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     8281 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     8341 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     8401 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     8461 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     8521 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     8581 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     8641 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     8701 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     8761 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     8821 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     8881 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     8941 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     9001 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     9061 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     9121 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     9181 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     9241 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     9301 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     9361 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     9421 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     9481 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     9541 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     9601 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     9661 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     9721 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     9781 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     9841 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     9901 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     9961 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
    10021 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
    10081 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
    10141 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
    10201 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
    10261 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
    10321 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    10381 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    10441 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    10501 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    10561 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    10621 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
    10681 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
    10741 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
    10801 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    10861 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    10921 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
    10981 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
    11041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
    11161 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    11221 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    11281 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
    11341 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    11401 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    11461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    11521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    11581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    11641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    11701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    11761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    11821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    12001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    12061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    12121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    12181 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    12241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    12301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    12361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    12421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    12481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    12541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    12601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    12661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    12721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    12781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    12841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    12901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    12961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    13021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    13081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    13141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    13201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    13261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    13321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    13381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    13441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    13501 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    13561 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    13621 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    13681 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    13741 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    13801 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    13861 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    13921 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
