LOCUS       V021717                10717 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021717
VERSION     V021717
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4759
                     /label="FGFR2(NM_000141)"
                     /note="FGFR2(NM_000141)"
                     /gene="FGFR2"
     CDS             4766..4831
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4876..5087
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5100..5368
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5397..5406
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5427..6089
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6159..6212
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6213..6812
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6813..7400
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7474..7707
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7779..7900
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7919..8054
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8092..8108)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8116..8132
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8140..8170)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8185..8206
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8494..9082)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9253..10113)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10114..10218)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10692..10708
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg tcagctgggg tcgtttcatc tgcctggtcg tggtcaccat
     2341 ggcaaccttg tccctggccc ggccctcctt cagtttagtt gaggatacca cattagagcc
     2401 agaagagcca ccaaccaaat accaaatctc tcaaccagaa gtgtacgtgg ctgcgccagg
     2461 ggagtcgcta gaggtgcgct gcctgttgaa agatgccgcc gtgatcagtt ggactaagga
     2521 tggggtgcac ttggggccca acaataggac agtgcttatt ggggagtact tgcagataaa
     2581 gggcgccacg cctagagact ccggcctcta tgcttgtact gccagtagga ctgtagacag
     2641 tgaaacttgg tacttcatgg tgaatgtcac agatgccatc tcatccggag atgatgagga
     2701 tgacaccgat ggtgcggaag attttgtcag tgagaacagt aacaacaaga gagcaccata
     2761 ctggaccaac acagaaaaga tggaaaagcg gctccatgct gtgcctgcgg ccaacactgt
     2821 caagtttcgc tgcccagccg gggggaaccc aatgccaacc atgcggtggc tgaaaaacgg
     2881 gaaggagttt aagcaggagc atcgcattgg aggctacaag gtacgaaacc agcactggag
     2941 cctcattatg gaaagtgtgg tcccatctga caagggaaat tatacctgtg tagtggagaa
     3001 tgaatacggg tccatcaatc acacgtacca cctggatgtt gtggagcgat cgcctcaccg
     3061 gcccatcctc caagccggac tgccggcaaa tgcctccaca gtggtcggag gagacgtaga
     3121 gtttgtctgc aaggtttaca gtgatgccca gccccacatc cagtggatca agcacgtgga
     3181 aaagaacggc agtaaatacg ggcccgacgg gctgccctac ctcaaggttc tcaaggccgc
     3241 cggtgttaac accacggaca aagagattga ggttctctat attcggaatg taacttttga
     3301 ggacgctggg gaatatacgt gcttggcggg taattctatt gggatatcct ttcactctgc
     3361 atggttgaca gttctgccag cgcctggaag agaaaaggag attacagctt ccccagacta
     3421 cctggagata gccatttact gcataggggt cttcttaatc gcctgtatgg tggtaacagt
     3481 catcctgtgc cgaatgaaga acacgaccaa gaagccagac ttcagcagcc agccggctgt
     3541 gcacaagctg accaaacgta tccccctgcg gagacaggta acagtttcgg ctgagtccag
     3601 ctcctccatg aactccaaca ccccgctggt gaggataaca acacgcctct cttcaacggc
     3661 agacaccccc atgctggcag gggtctccga gtatgaactt ccagaggacc caaaatggga
     3721 gtttccaaga gataagctga cactgggcaa gcccctggga gaaggttgct ttgggcaagt
     3781 ggtcatggcg gaagcagtgg gaattgacaa agacaagccc aaggaggcgg tcaccgtggc
     3841 cgtgaagatg ttgaaagatg atgccacaga gaaagacctt tctgatctgg tgtcagagat
     3901 ggagatgatg aagatgattg ggaaacacaa gaatatcata aatcttcttg gagcctgcac
     3961 acaggatggg cctctctatg tcatagttga gtatgcctct aaaggcaacc tccgagaata
     4021 cctccgagcc cggaggccac ccgggatgga gtactcctat gacattaacc gtgttcctga
     4081 ggagcagatg accttcaagg acttggtgtc atgcacctac cagctggcca gaggcatgga
     4141 gtacttggct tcccaaaaat gtattcatcg agatttagca gccagaaatg ttttggtaac
     4201 agaaaacaat gtgatgaaaa tagcagactt tggactcgcc agagatatca acaatataga
     4261 ctattacaaa aagaccacca atgggcggct tccagtcaag tggatggctc cagaagccct
     4321 gtttgataga gtatacactc atcagagtga tgtctggtcc ttcggggtgt taatgtggga
     4381 gatcttcact ttagggggct cgccctaccc agggattccc gtggaggaac tttttaagct
     4441 gctgaaggaa ggacacagaa tggataagcc agccaactgc accaacgaac tgtacatgat
     4501 gatgagggac tgttggcatg cagtgccctc ccagagacca acgttcaagc agttggtaga
     4561 agacttggat cgaattctca ctctcacaac caatgaggaa tacttggacc tcagccaacc
     4621 tctcgaacag tattcaccta gttaccctga cacaagaagt tcttgttctt caggagatga
     4681 ttctgttttt tctccagacc ccatgcctta cgaaccatgc cttcctcagt atccacacat
     4741 aaacggcagt gttaaaacag gatccgacta caaagaccat gacggtgatt ataaagatca
     4801 tgacatcgac tacaaggatg acgatgacaa gtgagcggcc gcgaaggatc tgcgatcgct
     4861 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     4921 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     4981 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     5041 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
     5101 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
     5161 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
     5221 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
     5281 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
     5341 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca
     5401 ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca
     5461 ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc
     5521 gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga
     5581 gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc
     5641 ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg
     5701 acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg
     5761 acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca
     5821 tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca
     5881 gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc
     5941 acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg
     6001 ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc
     6061 acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg
     6121 tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa
     6181 catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc
     6241 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6301 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6361 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6421 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6481 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6541 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6601 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6661 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6721 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6781 cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg
     6841 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6901 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6961 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7021 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7081 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7141 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7201 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7261 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7321 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct
     7381 ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag
     7441 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7501 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7561 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7621 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7681 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     7741 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7801 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7861 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7921 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7981 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8041 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct
     8101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10261 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10321 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10381 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10441 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10501 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10561 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10621 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10681 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
