LOCUS       V022473                11967 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022473
VERSION     V022473
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5995
                     /label="FBLN2(NM_001004019)"
                     /note="FBLN2(NM_001004019)"
                     /gene="FBLN2"
     CDS             6011..6076
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6126..6337
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6350..6618
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6647..6656
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6677..7339
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7409..7462
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7463..8062
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8063..8650
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8724..8957
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9029..9150
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9169..9304
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9342..9358)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9366..9382
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9390..9420)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9435..9456
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9744..10332)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10503..11363)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11364..11468)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11942..11958
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggtgct gctctgggag cctgcaggag cctggcttgc
     2341 tctgggcctg gccctggccc tgggccccag cgtggccgca gctgcccctc ggcaggactg
     2401 cacgggcgtg gagtgcccgc cgctggagaa ctgcattgag gaggcgctgg agccgggtgc
     2461 ctgctgtgcc acgtgtgtgc agcagggctg cgcctgcgag ggctaccagt actatgactg
     2521 cctacagggt ggcttcgtgc gcggccgcgt gcccgccggt cagtcctatt ttgtggactt
     2581 cgggagcact gagtgctcct gcccaccagg cggcggcaag atcagctgcc agttcatgct
     2641 gtgcccggag ctgccgccca actgcatcga ggctgtagtg gtggctgaca gctgcccaca
     2701 gtgcggccag gtgggctgcg tccacgcggg ccacaagtac gccgctggcc acactgttca
     2761 cctgccgccc tgccgggcct gccactgccc tgacgccggt ggagagctca tctgctacca
     2821 gctccccggt tgccacggga acttctcaga tgccgaggag ggtgaccccg agcgacacta
     2881 cgaagacccc tacagctatg accaggaggt ggccgaggtg gaagcagcaa cagccctggg
     2941 gggtgaggtc caggcgggtg cagtccaggc aggcgcaggg ggccccccag ctgctctggg
     3001 aggtgggagt cagccactgt ccaccatcca ggcacccccc tggccagctg tcctccccag
     3061 gcccacagcg gctgctgccc tgggtccccc agccccagtg caggccaaag ctaggagagt
     3121 gaccgaggac agtgaggagg aagaagagga ggaggaggag agagaggaaa tggctgtcac
     3181 tgagcagctg gcagcaggtg gccacagggg gctggatggg ctgcccacta cagccccagc
     3241 tggacccagt cttcctatcc aggaggagag ggcagaagct ggggcaaggg cagaagctgg
     3301 ggcaaggcct gaagagaacc tcatcctgga tgcccaagcc acgtcccgca gcactgggcc
     3361 ggagggcgtg acgcatgcac cgagcctggg caaggctgct ctcgtcccaa ctcaggccgt
     3421 gcctggctct cccagggacc cagtcaagcc cagcccccac aacatcctgt ccacatcact
     3481 gcctgatgca gcctggatcc cacccacccg agaagtgccc aggaagccgc aagttctgcc
     3541 ccattcccac gtggaggagg acacagaccc caactctgtc cattctatcc ccagaagtag
     3601 ccctgaaggc tccaccaagg acctgatcga gacttgctgc gcagccggac agcagtgggc
     3661 cattgacaat gacgagtgcc tggagatccc tgagagtggc actgaggaca acgtctgcag
     3721 gacagcccag aggcactgct gtgtctccta cttgcaggag aagagctgca tggccggcgt
     3781 cctgggagcc aaggagggtg agacctgtgg ggctgaggac aacgacagct gcggcatctc
     3841 cctgtacaag caatgctgtg actgctgtgg cctgggcctc cgcgtgcggg ccgagggcca
     3901 gtcgtgtgag tccaatccta acctgggcta tccctgcaat catgtcatgc tctcctgctg
     3961 tgagggtgaa gagcctctca tagtacctga ggttcgccga cctccagagc ccgcagctgc
     4021 accacggaga gtttcagagg cagagatggc gggccgagag gccctgtcac tgggcacaga
     4081 ggccgagctg ccgaacagcc tgccgggcga tgaccaggat gagtgccttc tcctcccggg
     4141 agagctgtgc cagcaccttt gcatcaatac tgtgggttct taccactgtg cctgctttcc
     4201 tggcttctca ctgcaggacg atggccgcac ttgccgccca gagggtcacc ctccacagcc
     4261 ggaagcccca caggagcctg cactgaagtc agaattttcc caggtggcct ctaacaccat
     4321 cccgctgcca ctgccgcagc ccaatacctg caaagacaat ggaccctgca agcaggtgtg
     4381 cagcactgtt gggggctcag ccatatgctc ctgttttccc ggctatgcca tcatggcgga
     4441 tggcgtgtcc tgtgaagacc aagacgagtg cctgatgggt gctcacgatt gtagccggcg
     4501 acagttctgt gtgaacaccc tgggatcctt ctactgtgtc aaccacacag tgctctgtgc
     4561 cgatggctat atcctcaatg cgcacaggaa gtgcgtggac atcaacgagt gtgtgacgga
     4621 cctgcacacg tgcagccggg gcgagcactg tgtgaacaca ctgggctcct tccactgcta
     4681 caaggcactc acctgtgagc caggctatgc cctcaaggat ggcgagtgcg aagacgtgga
     4741 tgagtgtgcg atgggcacgc acacctgcca gccgggcttc ttgtgccaga acaccaaggg
     4801 ctccttctac tgccaggcca ggcagcgctg catggatggc ttcctgcagg atcctgaagg
     4861 caactgtgtg gacatcaacg agtgcacgtc actgtccgag ccatgtcggc caggcttcag
     4921 ctgcatcaac acggtgggct cctacacatg ccagaggaac ccgctgatct gcgcgcgcgg
     4981 ctaccacgcc agcgatgatg ggaccaagtg tgtggacgtg aatgagtgtg agacaggtgt
     5041 gcaccgctgc ggtgagggcc aagtgtgcca caacctccct ggctcctacc gctgtgactg
     5101 caaagccggc tttcagcggg atgcctttgg ccggggctgc atcgacgtga atgagtgctg
     5161 ggcctcgcca ggccgcctgt gccagcacac gtgtgagaac acactcggct cctaccgctg
     5221 ttcctgcgcc tccgggttcc tgctagcagc ggacggcaag cgctgtgaag acgtgaatga
     5281 gtgtgaggcc cagcgctgca gccaggagtg tgccaacatc tatggctcct accagtgcta
     5341 ctgccgccag ggctaccagc tggctgagga tgggcacacc tgcacagaca tcgacgagtg
     5401 tgctcaaggc gccggcatcc tctgcacctt ccgctgtctc aacgtgccag ggagctacca
     5461 gtgtgcatgc cctgagcagg gctacaccat gacggccaac gggaggtcct gcaaggacgt
     5521 ggatgagtgt gcactgggta cccacaactg ttccgaggct gagacctgcc acaacatcca
     5581 gggtagcttc cgctgcctgc gcttcgagtg tcctcccaac tatgtccaag tctccaaaac
     5641 gaagtgcgag cgcaccacgt gccatgactt cctggagtgc cagaactcgc cagcgcgcat
     5701 cacgcactac cagctcaact tccagacggg cctcctggtg cctgcgcata tcttccgcat
     5761 tggccccgcg ccagccttca cgggggacac catcgccctg aacatcatca agggcaatga
     5821 ggagggctac tttggcacgc gcaggctcaa tgcctacacg ggtgtggtct acctgcagcg
     5881 ggccgtgctg gagccccggg actttgccct ggacgtggag atgaagctct ggaggcaggg
     5941 ctccgtcacc accttcctgg ccaagatgca catcttcttc accacctttg ccctgggtgg
     6001 cggcggttcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     6061 ggatgacgat gacaagtagg atccgcggcc gcgaaggatc tgcgatcgct ccggtgcccg
     6121 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     6181 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     6241 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     6301 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     6361 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     6421 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     6481 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     6541 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     6601 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag
     6661 cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg
     6721 cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa
     6781 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat
     6841 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca
     6901 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt
     6961 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt
     7021 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa
     7081 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg
     7141 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt
     7201 caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg
     7261 cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa
     7321 gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac
     7381 cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga
     7441 cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg
     7501 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7561 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7621 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7681 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7741 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7801 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     7861 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     7921 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     7981 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8041 gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac
     8101 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     8161 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8221 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     8281 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8341 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8401 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     8461 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8521 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8581 tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc
     8641 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8701 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct
     8761 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8821 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8881 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8941 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9001 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     9061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9121 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     9181 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9241 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9301 ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg
     9361 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     9421 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     9481 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     9541 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     9601 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9661 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9721 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9781 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9841 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9901 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9961 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10021 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10081 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10141 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    10201 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10261 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10321 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10381 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10441 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10501 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10561 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10621 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    10681 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10741 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10801 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10861 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10921 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10981 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11041 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11101 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11161 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11221 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11281 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11341 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11401 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11461 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    11521 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    11581 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    11641 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    11701 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    11761 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
    11821 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
    11881 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
    11941 tgtaaaacga cggccagtgc caagctg
//
