LOCUS       V037795                11359 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037795
VERSION     V037795
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5385
                     /label="FAM13A(NM_014883)"
                     /note="FAM13A(NM_014883)"
                     /gene="FAM13A"
     CDS             5392..5457
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5501..5712
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5725..5993
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6022..6031
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6052..6714
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6784..6837
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6838..7437
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7438..8026
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8100..8333
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8405..8526
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8566..8701
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8734..8750)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8758..8774
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8782..8812)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8827..8848
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9136..9724)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9895..10755)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10756..10860)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11334..11350
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gggcaggagc tctagccatc
     2341 tgtcaaagta aagcagcggt tcggctgaaa gaagacatga aaaagatagt ggcagtgcca
     2401 ttaaatgaac agaaggattt tacctatcag aagttatttg gagtcagtct ccaagaactt
     2461 gaacggcagg ggctcaccga gaatggcatt ccagcagtag tgtggaatat agtggaatat
     2521 ttgacgcagc atggacttac ccaagaaggt ctttttaggg tgaatggtaa cgtgaaggtg
     2581 gtggaacaac ttcgactgaa gttcgagagt ggagtgcccg tggagctcgg gaaggacggt
     2641 gatgtctgct cagcagccag tctgttgaag ctgtttctga gggagctgcc tgacagtctg
     2701 atcacctcag cgttgcagcc tcgattcatt caactctttc aggatggcag aaatgatgtt
     2761 caggagagta gcttaagaga cttaataaaa gagctgccag acacccacta ctgcctcctc
     2821 aagtaccttt gccagttctt gacaaaagta gccaagcatc atgtgcagaa tcgcatgaat
     2881 gttcacaatc tcgccactgt atttgggcca aattgctttc atgtgccacc tgggcttgaa
     2941 ggcatgaagg aacaggacct gtgcaacaag ataatggcta aaattctaga aaattacaat
     3001 accctgtttg aagtagagta tacagaaaat gatcatctga gatgtgaaaa cctggctagg
     3061 cttatcatag taaaagaggt ctattataag aactccctgc ccatcctttt aacaagaggc
     3121 ttagaaagag acatgccaaa accacctcca aaaaccaaga tcccaaaatc caggagtgag
     3181 ggatctattc aggcccacag agtactgcaa ccagagctat ctgatggcat tcctcagctc
     3241 agcttgcggc taagttatag aaaagcctgc ttggaagaca tgaattcagc agagggtgct
     3301 attagtgcca agttggtacc cagttcacag gaagatgaaa gacctctgtc acctttctat
     3361 ttgagtgctc atgtacccca agtcagcaat gtgtctgcaa ccggagaact cttagaaaga
     3421 accatccgat cagctgtaga acaacatctt tttgatgtta ataactctgg aggtcaaagt
     3481 tcagaggact cagaatctgg aacactatca gcatcttctg ccacatctgc cagacagcgc
     3541 cgccgccagt ccaaggagca ggatgaagtt cgacatggga gagacaaggg acttatcaac
     3601 aaagaaaata ctccttctgg gttcaaccac cttgatgatt gtattttgaa tactcaggaa
     3661 gtcgaaaagg tacacaaaaa tacttttggt tgtgctggag aaaggagcaa gcctaaacgt
     3721 cagaaatcca gtactaaact ttctgagctt catgacaatc aggacggtct tgtgaatatg
     3781 gaaagtctca attccacacg atctcatgag agaactggac ctgatgattt tgaatggatg
     3841 tctgatgaaa ggaaaggaaa tgaaaaagat ggtggacaca ctcagcattt tgagagcccc
     3901 acaatgaaga tccaggagca tcccagccta tctgacacca aacagcagag aaatcaagat
     3961 gccggtgacc aggaggagag ctttgtctcc gaagtgcccc agtcggacct gactgcattg
     4021 tgtgatgaaa agaactggga agagcctatc cctgctttct cctcctggca gcgggagaac
     4081 agtgactctg atgaagccca cctctcgccg caggctgggc gcctgatccg tcagctgctg
     4141 gacgaagaca gcgaccccat gctctctcct cggttctacg cttatgggca gagcaggcaa
     4201 tacctggatg acacagaagt gcctccttcc ccaccaaact cccattcttt catgaggcgg
     4261 cgaagctcct ctctggggtc ctatgatgat gagcaagagg acctgacacc tgcccagctc
     4321 acacgaagga ttcagagcct taaaaagaag atccggaagt ttgaagatag attcgaagaa
     4381 gagaagaagt acagaccttc ccacagtgac aaagcagcca atccggaggt tctgaaatgg
     4441 acaaatgacc ttgccaaatt ccggagacaa cttaaagaat caaaactaaa gatatctgaa
     4501 gaggacctaa ctcccaggat gcggcagcga agcaacacac tccccaagag ttttggttcc
     4561 caacttgaga aagaagatga gaagaagcaa gagctggtgg ataaagcaat aaagcccagt
     4621 gttgaagcca cattggaatc tattcagagg aagctccagg agaagcgagc ggaaagcagc
     4681 cgccctgagg acattaagga tatgaccaaa gaccagattg ctaatgagaa agtggctctg
     4741 cagaaagctc tgttatatta tgaaagcatt catggacggc cggtaacaaa gaacgaacgg
     4801 caggtgatga agccactata cgacaggtac cggctggtca aacagatcct ctcccgagct
     4861 aacaccatac ccatcattgg ttccccctcc agcaagcgga gaagcccttt gctgcagcca
     4921 attatcgagg gcgaaactgc ttccttcttc aaggagataa aggaagaaga ggaggggtca
     4981 gaagacgata gcaatgtgaa gccagacttc atggtcactc tgaaaaccga tttcagtgca
     5041 cgatgctttc tggaccaatt cgaagatgac gctgatggat ttatttcccc aatggatgat
     5101 aaaataccat caaaatgcag ccaggacaca gggctttcaa atctccatgc tgcctcaata
     5161 cctgaactcc tggaacacct ccaggaaatg agagaagaaa agaaaaggat tcgaaagaaa
     5221 cttcgggatt ttgaagacaa ctttttcaga cagaatggaa gaaatgtcca gaaggaagac
     5281 cgcactccta tggctgaaga atacagtgaa tataagcaca taaaggcgaa actgaggctc
     5341 ctggaggtgc tcatcagcaa gagagacact gattccaagt ccatgggatc cgactacaaa
     5401 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     5461 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5521 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5581 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5641 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5701 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5761 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5821 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5881 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5941 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     6001 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     6061 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     6121 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     6181 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     6241 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     6301 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     6361 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     6421 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     6481 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     6541 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     6601 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     6661 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     6721 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     6781 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     6841 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6901 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6961 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     7021 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     7081 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7141 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7201 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7261 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7321 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7381 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     7441 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7501 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7561 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7621 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7681 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7741 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7801 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7861 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7921 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7981 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     8041 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8101 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     8161 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8221 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8281 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8341 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8401 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8521 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     8581 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8641 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8701 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     8761 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8821 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8881 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8941 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9001 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9061 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9121 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9181 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9241 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9361 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9421 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9481 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9541 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9601 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9661 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9721 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9781 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9841 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9901 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9961 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10021 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10081 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10141 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10201 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10261 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10321 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10381 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10441 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10501 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10561 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10621 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10681 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10741 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10861 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10921 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10981 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    11041 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11101 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11161 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11221 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11281 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11341 gacggccagt gccaagctg
//
