LOCUS       V024353                10397 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024353
VERSION     V024353
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4440
                     /label="Exoc5(NM_207214)"
                     /note="Exoc5(NM_207214)"
                     /gene="Exoc5"
     CDS             4447..4512
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4556..4767
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4780..5048
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5077..5086
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5107..5769
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5839..5892
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5893..6492
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6493..7080
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7154..7387
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7459..7580
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7599..7734
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7772..7788)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7796..7812
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7820..7850)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7865..7886
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8174..8762)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8933..9793)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9794..9898)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10372..10388
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccacgacggc cgagctcttc
     2341 gaggagcctt ttgtggccga tgaatacatt gaacgtctcg tgtggagaac cccaggaggt
     2401 ggttctaggg gtggacctga agcttttgat cctaaaaggt tattagaaga atttgtgaat
     2461 catattcaag aactgcagat aatggatgaa aggattcaaa ggaaagtgga gaaattagaa
     2521 caacagtgtc agaaggaagc taaggagttt gctaagaagg tacaggagct ccagaaaagc
     2581 aaccaggttg ctttccaaca tttccaagag ctagatgagc acattagcta tgtagctacc
     2641 aaagtgtgtc accttggaga ccagttggaa ggggtaaaca cacctaggca acgtgcagtg
     2701 gaggcacaga aattgatgaa atactttaat gagtttctag atggagaatt gaaatctgat
     2761 gtttttacaa attctgaaaa gataaaggaa gcagcagacg tcattcaaaa actgcatctg
     2821 attgcccagg agttaccttt cgataggttt tcagaagtaa aatcaaaaat tgcaagtaaa
     2881 taccatgact tagaatgcca gctgattcag gagtttacaa gtgcccaaag aagaggggag
     2941 gtctcccgga tgagggaggt ggctgcagtc ctccttcatt ttaagggtta ttcccattgt
     3001 attgatgttt atataaagca gtgccaggag ggtgcttact tgagaaatga catatttgaa
     3061 gatgcagcaa tactctgtca gcgagtgaac aagcaggttg gagacatctt tagtaatcca
     3121 gaagcagtgc tggctaagct tattcagagt gtgtttgaaa tcaaactaca gagttttgtg
     3181 aaggatcagt tagaagaatg taggaaatct gatgctgagc agtatcttaa aagtctctat
     3241 gatctgtata caagaaccac cggtctttcc agcaaattga tggagtttaa cttaggtact
     3301 gataaacaga ctttcttgtc taagcttatc aaatccattt tcatttccta tctggagaac
     3361 tatattgaag tggagattgg atatttgaaa agcagaagtg ctatgatcct acagcgctat
     3421 tatgattcaa aaaaccacca aaagagatcc attggcacag gaggtattca agatttgaaa
     3481 gaaaggatta gacaacgtac caacttacca ctggggccaa gtattgatac acatggggaa
     3541 actttcctat ctcaagaagt ggtagttaat cttttacaag aaaccaaaca agcctttgaa
     3601 agatgtcata ggctttctga tccttctgat ttaccaagga atgcctttag aatttttacc
     3661 attcttgtgg aatttttatg tattgagcat attgattatg ctttagaaac gggtcttgct
     3721 ggaatcccat catcagattc taggaatgca aatctctact ttttggatgt tgtacaacag
     3781 gccaatacta tttttcatct ttttgacaaa cagtttaatg atcacctaat gccactaata
     3841 agctcttctc ctaagttatc tgaatgcctc cagaagaaaa aagagataat tgagcagatg
     3901 gagatgaaat tggatactgg cattgatagg acattaaatt gtatgattgg acagatgaag
     3961 catatcttgg ctgcagaaca aaagaaaaca gattttaagc cagaagatga gaacaatgtt
     4021 ttgattcagt atactaatgc atgtgtaaaa gtttgtgttt atgtaagaaa acaagttgag
     4081 aagattaaaa attccatgga tgggaagaat gtggacacag ttctgatgga acttggagta
     4141 cgttttcatc ggcttattta tgaacatctt caacaatatt cctatagttg tatgggtggc
     4201 atgcttgcaa tttgcgacgt ggctgagtat agaaaatgtg ccaaagactt caagattcca
     4261 atggtattac atctttttga tactctgcat gcactttgca atctcctggt cgtggctcca
     4321 gataatttaa aacaagtctg ctcgggagaa caacttgcca atctggacaa gaacatactc
     4381 cactcctttg tacaacttcg tgctgactat agatctgccc gcctggcgcg acacttcagc
     4441 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4501 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4561 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4621 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4681 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4741 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4801 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     4861 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     4921 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     4981 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5041 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5101 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5161 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5221 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5281 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5341 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5401 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5461 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5521 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5581 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5641 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5701 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5761 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     5821 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     5881 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5941 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6001 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6061 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6121 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6181 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6241 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6301 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6361 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6421 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6481 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6541 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6601 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6661 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6721 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6781 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6841 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6901 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6961 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7021 cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc
     7081 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7141 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     7201 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7261 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7321 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7381 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7441 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7501 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7561 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7681 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7741 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7801 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7861 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7921 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7981 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8341 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8581 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8821 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8881 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8941 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9001 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9061 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9121 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9181 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9241 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9301 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9361 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9421 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9481 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9541 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9601 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9661 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9721 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9781 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9841 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9901 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9961 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    10021 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10081 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    10141 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10201 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    10261 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    10321 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    10381 cggccagtgc caagctg
//
