LOCUS       V024252                12041 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024252
VERSION     V024252
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6084
                     /label="Erbb2(NM_001003817)"
                     /note="Erbb2(NM_001003817)"
                     /gene="Erbb2"
     CDS             6091..6156
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6200..6411
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6424..6692
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6721..6730
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6751..7413
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7483..7536
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7537..8136
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8137..8724
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8798..9031
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9103..9224
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9243..9378
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9416..9432)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9440..9456
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9464..9494)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9509..9530
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9818..10406)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10577..11437)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11438..11542)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12016..12032
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agctggcggc ctggtgccgt
     2341 tgggggttcc tcctcgccct cctgtccccc ggagccgcgg gtacccaagt gtgtaccggt
     2401 accgacatga agttgcgact ccctgccagt cctgagaccc acctggacat gcttcgccac
     2461 ctctaccagg gctgtcaggt ggtgcagggc aatttggagc ttacctacct gcccgccaat
     2521 gccagcctct cattcctgca ggacatccag gaagtccagg gatacatgct catcgctcac
     2581 aaccgagtga aacacgtccc actgcagagg ttgcgcatcg tgagagggac tcagctcttt
     2641 gaggacaagt atgccctggc tgtgctagac aaccgagacc ctttggacaa cgtcaccacc
     2701 gccgccccag gcagaacccc agaagggctg cgggagctgc agcttcgaag tctcacagag
     2761 atcttgaagg gaggagtttt gatccgtggg aaccctcagc tctgctacca ggacatggtt
     2821 ttgtggaagg atgtcctccg taagaataac cagctggctc ctgtcgacat ggacaccaat
     2881 cgttcccggg cctgtccacc ttgtgcccca acctgcaaag acaatcactg ttggggtgag
     2941 agtcctgaag actgtcagat cttgactggc accatctgta ctagtggctg tgcccggtgc
     3001 aagggccggc tgcccactga ctgttgccat gagcagtgtg ctgcaggctg cacgggtccc
     3061 aagcattctg actgcctggc ctgcctccac ttcaatcata gtggtatctg tgagctgcac
     3121 tgcccggccc tcatcaccta caacacagac accttcgagt ccatgctcaa ccctgagggt
     3181 cgctacacct ttggtgccag ctgtgtgacc acctgcccct acaactacct ctccacggaa
     3241 gtgggatcct gcactctggt ctgtcccccg aacaaccaag aggtcacagc tgaggacgga
     3301 acacagcggt gtgagaaatg cagcaagccc tgtgctggag tatgctatgg tctgggcatg
     3361 gagcacctcc gaggggcgag ggccatcacc agtgacaata tccaggagtt tgctggctgc
     3421 aagaagatct ttgggagcct ggcatttttg ccggagagct ttgatgggaa cccctcctcc
     3481 ggcgttgccc cactgaagcc agagcatctc caagtgttcg aaaccctgga ggagatcaca
     3541 ggttacctat acatttcagc atggccagag agcttccaag acctcagtgt cttccagaac
     3601 cttcgggtca ttcggggacg gattctccat gatggtgctt actcattgac gttgcaaggc
     3661 ctggggattc actcactggg gctacgctca ctgcgggagc tgggcagtgg attggctctc
     3721 attcaccgca acacccatct ctgctttgta aacactgtac cttgggacca gctcttccgg
     3781 aacccgcacc aggccctact ccacagtggg aaccggccag aagaggcatg tggtcttgag
     3841 ggcttggtct gtaactcact gtgtgcccgt gggcactgct gggggccagg gcccacccag
     3901 tgtgtcaact gcagtcagtt cctccggggc caggagtgtg tggaggagtg ccgagtatgg
     3961 aaggggctcc ccagggagta tgtgaggggc aagcactgtc tgccatgcca ccccgagtgt
     4021 cagcctcaaa acagctcgga gacctgctat ggatcggagg ctgaccagtg tgaggcttgt
     4081 gcccactaca aggactcatc ttcctgtgtg gctcgctgcc ccagtggtgt gaagccagac
     4141 ctctcctaca tgcctatctg gaagtacccg gatgaggagg gcatatgtca gccatgcccc
     4201 atcaactgca cccactcatg tgtggacctg gacgaacgag gctgcccagc agagcagaga
     4261 gccagcccag tgacattcat cattgcaact gtggtgggcg tcctgttgtt cctgatcata
     4321 gtggtggtca ttggaatcct aatcaaacga aggcgacaga agatccggaa gtataccatg
     4381 cgtaggctgc tgcaggagac cgagctggtg gagccgctga cgcccagtgg agctgtgccc
     4441 aaccaggctc agatgcggat cctaaaggag acagagctaa ggaagctgaa ggtgcttggg
     4501 tcaggagcct tcggcactgt ctacaagggc atctggatcc cagatgggga gaacgtgaaa
     4561 atccccgtgg ccatcaaggt gttgagggaa aacacatctc ctaaagctaa caaagaaatc
     4621 ctagatgaag cgtacgtcat ggctggtgtg ggttctccat atgtgtcccg cctcctgggc
     4681 atctgcctga catccacagt gcagctggtg acacagctta tgccctatgg ctgccttctg
     4741 gaccatgtcc gagaacaccg aggtcgctta ggctcccagg acctgctcaa ctggtgtgtt
     4801 cagattgcca aggggatgag ctacctggag gaagttcggc ttgttcacag ggacctagct
     4861 gcccgaaacg tgctagtcaa gagtcccaac cacgtcaaga ttaccgactt cgggctggca
     4921 cggctgctgg acattgatga gactgaatac catgcagatg ggggcaaggt gcccatcaag
     4981 tggatggcat tggaatctat tctcagacgc cggttcaccc atcagagtga tgtgtggagc
     5041 tatggtgtga ctgtgtggga gctgatgacc tttggggcca aaccttacga tgggatccca
     5101 gctcgggaga tccctgattt gctggagaag ggagaacgcc tacctcagcc tccaatctgc
     5161 accatcgacg tctacatgat catggtcaaa tgttggatga ttgactccga atgtcgcccg
     5221 agattccggg agttggtatc agaattctcc cgtatggcaa gggaccccca gcgctttgtg
     5281 gtcatccaga acgaggactt aggcccctcc agccccatgg acagcacctt ctaccgttca
     5341 ctgctggagg atgatgacat gggggagctg gtcgatgctg aagagtacct ggtaccccag
     5401 cagggattct tctccccaga ccctgcccta ggtactggga gcacagccca ccgcagacac
     5461 cgcagctcgt cggccaggag tggcggtggt gagctgacac tgggcctgga gccctcggaa
     5521 gaagagcccc ccagatctcc actggctccc tccgaagggg ctggctccga tgtgtttgat
     5581 ggtgacctgg cagtgggggt aaccaaagga ctgcagagcc tctctccaca tgacctcagc
     5641 cctctacagc ggtacagtga ggatcccaca ttacctctgc cccccgagac tgatggctac
     5701 gttgctcccc tggcctgcag cccccagccc gagtatgtga accagccaga ggttcggcct
     5761 cagtctccct tgaccccaga gggtcctccg cctcccatcc gacctgctgg tgctactcta
     5821 gaaagaccca agactctctc tcctgggaaa aatggggttg tcaaagacgt ttttgccttt
     5881 gggggtgctg tggagaaccc tgaatactta gcacccagag caggcactgc ctctcagccc
     5941 cacccttctc ctgccttcag cccagccttt gacaacctct attactggga ccagaactca
     6001 tcggagcagg gtcctccacc aagtaccttt gaagggaccc ccactgcaga gaaccctgag
     6061 tacctaggcc tggatgtgcc agtaggatcc gactacaaag accatgacgg tgattataaa
     6121 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     6181 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     6241 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     6301 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     6361 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     6421 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     6481 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     6541 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     6601 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     6661 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     6721 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     6781 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     6841 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     6901 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     6961 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     7021 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     7081 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     7141 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     7201 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     7261 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     7321 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     7381 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     7441 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     7501 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     7561 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     7621 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     7681 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     7741 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7801 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7861 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7921 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7981 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     8041 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     8101 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     8161 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8221 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8281 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8341 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8401 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8461 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8521 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     8581 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8641 ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg
     8701 atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     8761 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     8821 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8881 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8941 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9001 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     9061 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     9121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     9241 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9301 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9361 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     9421 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9481 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9541 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9601 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9661 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9721 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9781 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9841 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9901 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9961 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10021 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10081 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10141 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10201 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10261 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10321 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10381 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10441 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10501 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10561 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10621 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10681 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10741 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10801 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10861 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10921 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10981 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11041 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11101 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11161 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11221 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11281 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11341 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11401 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11461 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11521 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    11581 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    11641 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    11701 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    11761 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    11821 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    11881 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    11941 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    12001 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
