LOCUS       V038766                10299 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038766
VERSION     V038766
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4477
                     /label="Elmo1(NM_080288)"
                     /note="Elmo1(NM_080288)"
                     /gene="Elmo1"
     CDS             4508..5227
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5269..5480
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5493..5761
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5788..6387
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6394..6982
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7056..7289
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7361..7482
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7501..7636
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7674..7690)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7698..7714
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7722..7752)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7767..7788
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8076..8664)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8835..9695)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9696..9800)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10274..10290
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgc cgccgccttc ggacatcgtg aaggtggcca tagaatggcc
     2341 tggcgcctac cctaaactca tggagattga ccagaaaaag ccactgtctg ccattataaa
     2401 ggaagtctgt gatgggtggt ctcttgccaa ccatgaatat tttgcactgc agcacgctga
     2461 tagttcaaac ttctacatca cagaaaagaa tcgcaacgaa ataaaaaatg gcacgatcct
     2521 tcgattaacc acatctccag ctcagaatgc ccagcagctc catgaacgaa tacagtcatc
     2581 aagtatggat gccaagttgg aagccctgaa ggacttggcc agcctctccc gggatgttac
     2641 ctttgctcag gagttcataa acctggatgg catctcactt ctcactcaga tggtggaaag
     2701 tggcactgaa cgataccaga agttgcagaa gattatgaag ccctgctttg gagacatgct
     2761 gtccttcact ctcacagcct ttgtggagtt gatggaccac ggcattgtat catgggatac
     2821 attctcagtg gccttcatta agaagatagc cagctttgtg aacaagtctg ccatagacat
     2881 ctcaatcctc cagcggtcct tggccattct ggagtcaatg gttctcaaca gtcatgacct
     2941 ctaccaaaag gtagcccagg agatcaccat tggccaactc attcctcatc ttcaggggac
     3001 agaccaagaa atacaaactt acaccattgc tgtaatcaac gcacttttcc tcaaagctcc
     3061 tgatgagagg agacaggaga tggcgaatat tttggctcag aagcagctgc gctacatcat
     3121 tttaacacat gtcatccgag cacagcgggc catcaacaat gagatggcac accagttata
     3181 tgttctccaa gtgctgacct ttaaccttct ggaagacagg atgatgacta agatggaccc
     3241 ccaggaccag gcccaaaggg acatcatatt tgaacttcga agaattgctt tcgatgcaga
     3301 gtcagaacct aataacagca gtggcagcat ggagaaacgc aagtccatgt acactcggga
     3361 ttataaaaaa cttggcttca ttaatcacgt caatcctgcc atggacttca cacagacccc
     3421 tcctgggatg ctggctctgg acaacatgct gtatttcgct aagcaccacc aggatgcata
     3481 catccggatc gtgctagaga acagcagccg agaagataaa catgagtgcc ccttcggccg
     3541 cagcagtata gagctgacca agatgctgtg tgagatcctc aaagtgggcg agctgcctag
     3601 tgagacctgc aacgactttc acccgatgtt cttcacccat gacagatctt ttgaggaatt
     3661 cttctgcatt tgcattcaac tcctgaacaa aacatggaag gaaatgaggg caacatctga
     3721 agacttcaac aaggtaatgc aggtggtgaa ggagcaggtt atgagagcgc ttacaaccaa
     3781 gcctagttcc ctggaccagt tcaaaagcaa gctgcaaaac ctgagctaca ccgagatcct
     3841 gaaaatccgc cagtctgaga ggatgaacca ggaagatttc cagtctcgcc cgattttgga
     3901 actaaaggag aagatccagc cagaaatctt agagctgatt aaacagcagc gcctgaaccg
     3961 ccttgtggaa gggacctgct ttaggaaact caatgctcgc cggagacaag acaagttttg
     4021 gtattgtcgg ctttcaccaa atcacaaggt cttacattat ggcgacttgg aagagagccc
     4081 ccaaggagaa gtgccccacg attccctgca ggacaaattg ccggtggcag atatcaaagc
     4141 agtggtgacg ggaaaggact gccctcatat gaaagagaaa ggtgccctta aacaaaacaa
     4201 ggaggtgctt gaacttgctt tctccatctt atacgactca aattgccaac tgaacttcat
     4261 tgctcctgat aagcatgagt actgcatctg gacagatggg ctgaatgcac tgcttgggaa
     4321 ggacatgatg agtgacctga cacgcaatga cctggacacc ctgctgagca tggagatcaa
     4381 gcttcgcctg ctggacctgg aaaacatcca gatcccggat gcacctccgc ctatccccaa
     4441 ggaacctagc aactatgact ttgtctatga ctgtaacggt ggcggaggat ccggaggagg
     4501 gggaagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4561 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4621 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4681 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     4741 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4801 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4861 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4921 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4981 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5041 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5101 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5161 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5221 caagtaagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     5281 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     5341 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     5401 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     5461 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     5521 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     5581 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     5641 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     5701 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     5761 ccaagctgtg accggcgcct acgctagatg accgagtaca agcccacggt gcgcctcgcc
     5821 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5881 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5941 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6001 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6061 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6121 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6181 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6241 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6301 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6361 tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac ctctggatta caaaatttgt
     6421 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6481 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6541 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6601 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6661 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6721 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6781 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6841 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6901 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6961 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     7021 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7081 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7261 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7321 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7381 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7441 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7501 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7561 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7621 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
     7681 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7741 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7801 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7861 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7921 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7981 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8581 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8701 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8761 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8821 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8881 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8941 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9001 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9061 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     9841 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     9901 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     9961 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    10021 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10081 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    10141 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    10201 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    10261 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
