LOCUS       V037033                13234 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037033
VERSION     V037033
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7260
                     /label="Eif2ak4(NM_013719)"
                     /note="Eif2ak4(NM_013719)"
                     /gene="Eif2ak4"
     CDS             7267..7332
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7376..7587
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7600..7868
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7897..7906
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7927..8589
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8659..8712
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8713..9312
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9313..9901
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9975..10208
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10280..10401
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10441..10576
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10609..10625)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10633..10649
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10657..10687)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10702..10723
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11011..11599)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11770..12630)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12631..12735)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13209..13225
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctgggggccg cggggcctct
     2341 ggacgcggcc gggctgagcc acaggagagc tattcgcagc gacaggacca cgagctgcag
     2401 gccctggagg ccatttacgg ctcggacttc caggatctgc ggccggatgc gcgcggacgg
     2461 gtgagagagc ctcctgaaat caacttagtt ctttaccctc agggcctagc tggtgaagaa
     2521 gtatacgtgc aagtggaact gagggttaaa tgcccaccta catacccaga tgtagttccc
     2581 gaaatagact taaaaaatgc taaaggcctg tcgaatgaaa gtgttaattt gttaaaatca
     2641 cacctggaag aactggccaa aaagcagtgt ggagaggtga tgatatttga actggcacac
     2701 catgtgcagt cgtttctcag cgagcataac aagcccccac caaagtcttt ccatgaagaa
     2761 atgctggaaa ggcaggctca ggagaagcag cagaggttgc tggaggccag gcggaaggag
     2821 gagcaggagc aacgtgaaat cctccacgag attcagagaa ggaaagagga gatcaaggaa
     2881 gagaagaaaa ggaaggaaat ggctaagcag gaacgtttgg aaatcactag cttgacaaac
     2941 caggactatg cctctaagag agacccagca gggcacaggg cagctgccat cctccatgga
     3001 ggctctcctg actttgtagg aaatggtaaa gcccggacat actcctcagg aaggtccagg
     3061 cgagaacgtc agtattctgt gtgtagtggt gaaccctctc ctggctcgtg tgacatcctc
     3121 catttctctg tgggtagccc tgatcagctc atggtgcaca aagggagatg tgttggcagt
     3181 gatgagcagc tgggaaaggt ggtatacaat gctttggaaa cagccacggg cagctttgtc
     3241 ttgctgcatg agtgggtcct tcaatggcag aagatgggcc cttgccttac cagtcaagag
     3301 aaagagaaga ttgacaagtg caaaaggcag attcaaggag cagaaacaga attcagctcc
     3361 ctagtgaaac tgagccatcc aaatatcgtc cgctactttg cgatgaactc cagagaggag
     3421 gaggactcca ttgtgatcga cattctggca gagcacgtca gtggcatctc tctggctaca
     3481 cacctgagcc actcgggccc agtccccgcc caccagctgc gcaagtacac agcccagctc
     3541 ctggcaggcc tggattacct acacagcaac tccgtggtgc acaaggttct gagcgcgtcc
     3601 agtgtcttgg tagatgccga gggcactgtc aagataacag actacagcat ctctaagcgt
     3661 ctggcagaca tttgcaagga ggatgtattt gagcaagctc gagttcgttt tagtgacagt
     3721 gccctgcctt ataaaacagg aaagaaaggg gatgtgtggc ggctcggcct cctgctgttg
     3781 tctctcagcc aaggacagga gtgtggggag tatccggtga ccatccccag tgacctgcca
     3841 gctgacttcc aagacttcct gaagaagtgt gtctgcctgg acgacaagga aagatggagc
     3901 cctcagcagc tgctgaaaca cagctttata aaccctcaac caaagctgcc tttggtggag
     3961 cagagtcctg aagactctgg gggacaagac tacattgaga ccgtcattcc cagcaaccag
     4021 ctgcccagcg ctgcgttctt cagtgagaca cagaaacagt tctcccggta cttcattgag
     4081 tttgaagaac tacaactcct agggaaagga gcttttggag ctgtcatcaa ggtgcaaaac
     4141 aagcttgatg gctgctgcta tgctgtgaag cgcatcccta tcaaccctgc aagcagacac
     4201 ttccgccgga tcaagggcga ggtgacactg ctatcgcgcc tgcaccatga gaacattgtg
     4261 cgctactaca acgcctggat cgagcggcat gagcgcccag cggtgccagg gacaccgccc
     4321 ccagactgca caccccaggc ccaggacagc ccagccacct gcgggaaaac atcaggcgac
     4381 actgaagaac tgggcagcgt ggaggctgca gcaccgccac ccatcctcag cagctcggtg
     4441 gagtggagca catctgcaga gcgttctacc agcacccgct tcccagtcac gggccaggat
     4501 tccagcagcg atgaggagga cgaggacgag cgcgatggcg tcttctccca gtcctttcta
     4561 cctgcttcag attctgacag tgacatcatc tttgacaatg aagatgaaaa cagtaaaagt
     4621 caaaatcagg atgaagactg caatcaaaag gacggtagcc atgagattga gccttcagtg
     4681 acagctgagg ctgtgcacta cctttatatc cagatggagt actgcgagaa gagcacgctg
     4741 cgggacacca ttgaccaggg gctgttccga gacaccagcc ggctctggag gcttttccga
     4801 gagattctgg atggattagc ttatatccat gagaaaggaa tgattcatcg cgacttgaag
     4861 cctgtcaata tttttttgga ttctgatgac catgtgaaaa taggtgactt tggcctggca
     4921 acagaccatc tggccttcac tgctgaaggt aaacaggacg accaggcagg tgacggagtg
     4981 attaagtcag acccttcagg ccatttgact ggcatggttg gtactgctct gtatgtaagc
     5041 cctgaggtcc aaggaagcac caagtctgca tacaaccaga aagtggatct cttcagcctg
     5101 ggaattatct tctttgagat gtcttatcac ccgatggtga ctgcctcaga aagaattttc
     5161 gttctcaacc aacttcgaga tcccacatcg cccaagtttc cagacgactt cgatgatgga
     5221 gaacatacaa agcagaaatc tgtcatctcc tggctgttga accatgatcc agctaaacgg
     5281 cccacggcca tggaactgct caagagtgag ctgctccccc cgccgcagat ggaggagtcc
     5341 gagctgcatg aagtgctgca ccacacgctg gccaacatag atgggaaggc ctaccgcacc
     5401 atgatgagtc agatcttctg ccagcacatc tcccccgcta tagactacac ctacgacagt
     5461 gacatcctga agggcaactt cttgattcgt acagccaaga tccagcagct tgtatgtgaa
     5521 accatcgtcc gcgtctttaa aaggcatggc gctgtccagc tgtgcacccc actgctgctt
     5581 ccccgaaaca ggcaaatata tgagcacaac gaagcggctt tgttcatgga tcacagcggc
     5641 atgctggtga tgcttccgtt cgacctgcgg gtcccttttg caagatatgt ggcaagaaat
     5701 aacatactga atttaaaacg atactgcata gagcgggtgt tcagacctcg gaagttagac
     5761 cgatttcatc ccaaagaact cctggaatgt gcatttgata tcgtcacttc taccaccaac
     5821 agctctctgc ccactgccga aaccatctac accatctatg aaataatcca ggagtttcct
     5881 gcacttcagg aaaggaatta cagcatttac ttgaaccata cgatgcttct gaaggcaata
     5941 ctcctacact gtgggatccc agaggacaag ctcagccaag tctacgtcat tctgtatgat
     6001 gctgtgacag agaagctgac taggagagaa gtggaagcta aattctgtaa cctgtccttg
     6061 tcttctaata gcctgtgtag actctacaag ttcattgagc agaaggggga tttgcaagac
     6121 ttaacgccaa ccatcaactc actaataaaa cagaaaacag gcgttgctca gttggtgaag
     6181 tatagcttaa aagacctcga ggacgtcgtc gggctgctga agaaacttgg cgtgaagtta
     6241 caggtctcca tcaacctggg cttggtctac aaggtgcagc agcacactgg catcatcttc
     6301 cagttcctgg cattcagcaa acgcaggcaa agggttgtgc ctgagatcct tgccgccggt
     6361 ggcagatacg acctgctgat tcccaagttc agaggcccac agactgtggg gccagtcccc
     6421 actgctgtcg gtgtcagcat agccatagac aagatatttg ctgctgtcct caacatggaa
     6481 gagcctgtta cagtgagctc ctgtgacctc ctggttgtca gtgttggcca gatgtccatg
     6541 tccagggcca tcaacctaac ccagaaactg tggacagcgg gcatcactgc agaaatcatg
     6601 tatgactggt cccagtccca ggaagagtta caagagtact gcagacacca tgaaatcacc
     6661 tatgtggctc tggtctccga taaagaagga agccatgtca aggtcaagtc ctttgagaag
     6721 gagaggcaaa cagaaaagcg tgtattggaa tcggatcttg tggatcacgt tatgcagaaa
     6781 ctaagaacca aagttggtga cgaaagaaat ttcagagacg cttctgataa tcttgccgta
     6841 caaactctga aggggtcgtt ttctaatgct tcaggtttgt ttgaaatcca tggaactaca
     6901 gtagtcccca acgtgattgt tctagcacca gaaaagctgt cagccagcac caggaggcga
     6961 catgagattc aggtgcaaac ccgacttcag actacacttg ccaacttaca tcagaaaagc
     7021 agtgaaattg aaattttggc tgtggaccta cccaaggaaa caatcttaca gtttctatca
     7081 ttagagtggg atgctgatga acaggcattt aacacaactg tgaagcagct gctgtcacgc
     7141 ctgccaaagc aaagatacct caaactcgtc tgcgatgaaa tttataacat caaagttgaa
     7201 aagaaggtgt cagtgctgtt cctgtacagc tacagagatg actactacag aatcctattt
     7261 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     7321 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     7381 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     7441 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     7501 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     7561 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     7621 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     7681 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     7741 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     7801 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     7861 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     7921 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     7981 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     8041 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     8101 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     8161 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     8221 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     8281 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     8341 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     8401 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     8461 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     8521 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     8581 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     8641 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     8701 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8761 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8821 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8881 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8941 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     9001 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     9061 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     9121 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     9181 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     9241 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     9301 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     9361 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     9421 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     9481 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9541 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9601 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9661 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     9721 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9781 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9841 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9901 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9961 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
    10021 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    10081 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    10141 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    10201 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
    10261 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
    10321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10381 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
    10441 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10501 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10561 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
    10621 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10681 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10741 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10801 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10921 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10981 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    11041 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11101 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    11161 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11221 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11281 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11341 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11401 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    11461 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11521 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11641 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11701 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11761 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11821 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11881 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11941 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    12001 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    12061 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    12121 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    12181 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    12241 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12301 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12361 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12421 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12481 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12541 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12601 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12661 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12721 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    12781 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    12841 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    12901 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    12961 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    13021 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    13081 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    13141 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    13201 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
