LOCUS       V044610                10373 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044610
VERSION     V044610
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4554
                     /label="EZH2(NM_004456)"
                     /note="EZH2(NM_004456)"
                     /gene="EZH2"
     CDS             4591..5301
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        5343..5554
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5567..5835
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5862..6461
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6468..7056
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7130..7363
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7435..7556
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7575..7710
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7748..7764)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7772..7788
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7796..7826)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7841..7862
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8150..8738)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8909..9769)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9770..9874)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10348..10364
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgggccag actgggaaga aatctgagaa gggaccagtt
     2341 tgttggcgga agcgtgtaaa atcagagtac atgcgactga gacagctcaa gaggttcaga
     2401 cgagctgatg aagtaaagag tatgtttagt tccaatcgtc agaaaatttt ggaaagaacg
     2461 gaaatcttaa accaagaatg gaaacagcga aggatacagc ctgtgcacat cctgacttct
     2521 gtgagctcat tgcgcgggac tagggagtgt tcggtgacca gtgacttgga ttttccaaca
     2581 caagtcatcc cattaaagac tctgaatgca gttgcttcag tacccataat gtattcttgg
     2641 tctcccctac agcagaattt tatggtggaa gatgaaactg ttttacataa cattccttat
     2701 atgggagatg aagttttaga tcaggatggt actttcattg aagaactaat aaaaaattat
     2761 gatgggaaag tacacgggga tagagaatgt gggtttataa atgatgaaat ttttgtggag
     2821 ttggtgaatg cccttggtca atataatgat gatgacgatg atgatgatgg agacgatcct
     2881 gaagaaagag aagaaaagca gaaagatctg gaggatcacc gagatgataa agaaagccgc
     2941 ccacctcgga aatttccttc tgataaaatt tttgaagcca tttcctcaat gtttccagat
     3001 aagggcacag cagaagaact aaaggaaaaa tataaagaac tcaccgaaca gcagctccca
     3061 ggcgcacttc ctcctgaatg tacccccaac atagatggac caaatgctaa atctgttcag
     3121 agagagcaaa gcttacactc ctttcatacg cttttctgta ggcgatgttt taaatatgac
     3181 tgcttcctac atcgtaagtg caattattct tttcatgcaa cacccaacac ttataagcgg
     3241 aagaacacag aaacagctct agacaacaaa ccttgtggac cacagtgtta ccagcatttg
     3301 gagggagcaa aggagtttgc tgctgctctc accgctgagc ggataaagac cccaccaaaa
     3361 cgtccaggag gccgcagaag aggacggctt cccaataaca gtagcaggcc cagcaccccc
     3421 accattaatg tgctggaatc aaaggataca gacagtgata gggaagcagg gactgaaacg
     3481 gggggagaga acaatgataa agaagaagaa gagaagaaag atgaaacttc gagctcctct
     3541 gaagcaaatt ctcggtgtca aacaccaata aagatgaagc caaatattga acctcctgag
     3601 aatgtggagt ggagtggtgc tgaagcctca atgtttagag tcctcattgg cacttactat
     3661 gacaatttct gtgccattgc taggttaatt gggaccaaaa catgtagaca ggtgtatgag
     3721 tttagagtca aagaatctag catcatagct ccagctcccg ctgaggatgt ggatactcct
     3781 ccaaggaaaa agaagaggaa acaccggttg tgggctgcac actgcagaaa gatacagctg
     3841 aaaaaggacg gctcctctaa ccatgtttac aactatcaac cctgtgatca tccacggcag
     3901 ccttgtgaca gttcgtgccc ttgtgtgata gcacaaaatt tttgtgaaaa gttttgtcaa
     3961 tgtagttcag agtgtcaaaa ccgctttccg ggatgccgct gcaaagcaca gtgcaacacc
     4021 aagcagtgcc cgtgctacct ggctgtccga gagtgtgacc ctgacctctg tcttacttgt
     4081 ggagccgctg accattggga cagtaaaaat gtgtcctgca agaactgcag tattcagcgg
     4141 ggctccaaaa agcatctatt gctggcacca tctgacgtgg caggctgggg gatttttatc
     4201 aaagatcctg tgcagaaaaa tgaattcatc tcagaatact gtggagagat tatttctcaa
     4261 gatgaagctg acagaagagg gaaagtgtat gataaataca tgtgcagctt tctgttcaac
     4321 ttgaacaatg attttgtggt ggatgcaacc cgcaagggta acaaaattcg ttttgcaaat
     4381 cattcggtaa atccaaactg ctatgcaaaa gttatgatgg ttaacggtga tcacaggata
     4441 ggtatttttg ccaagagagc catccagact ggcgaagagc tgttttttga ttacagatac
     4501 agccaggctg atgccctgaa gtatgtcggc atcgaaagag aaatggaaat ccctggatcc
     4561 ggtggaggcg gatcaggagg cggtggatct atggtgagca agggcgagga ggataacatg
     4621 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     4681 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     4741 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     4801 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     4861 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     4921 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     4981 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     5041 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     5101 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     5161 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     5221 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     5281 atggacgagc tgtacaagta ggcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     5341 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     5401 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     5461 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     5521 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     5581 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     5641 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     5701 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     5761 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     5821 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gatgaccgag tacaagccca
     5881 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     5941 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     6001 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6061 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6121 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6181 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6241 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6301 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6361 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6421 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg
     6481 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6541 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6601 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6661 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6721 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6781 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6841 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6901 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6961 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7021 agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact
     7081 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7141 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7201 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7261 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7321 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7381 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7501 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7561 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7621 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7681 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     7741 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7801 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7861 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7921 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7981 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8041 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8101 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8161 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8221 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8281 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8341 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8401 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8461 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8521 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8581 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8641 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8701 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8761 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8821 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8881 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8941 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9001 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9061 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9121 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9181 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9241 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9301 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9361 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9421 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9481 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9541 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9601 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9661 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9721 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9781 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9841 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9901 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9961 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10021 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10081 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10141 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10201 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10261 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10321 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
