LOCUS       V032858                11221 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032858
VERSION     V032858
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5247
                     /label="ERN1(NM_001433)"
                     /note="ERN1(NM_001433)"
                     /gene="ERN1"
     CDS             5254..5319
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5363..5574
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5587..5855
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5884..5893
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5914..6576
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6646..6699
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6700..7299
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7300..7888
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7962..8195
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8267..8388
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8428..8563
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8596..8612)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8620..8636
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8644..8674)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8689..8710
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8998..9586)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9757..10617)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10618..10722)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11196..11212
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cggcccggcg gctgctgctg
     2341 ctgctgacgc tgctgctgcc cggcctcggg atttttggaa gtaccagcac agtgacgctt
     2401 cctgaaacct tgttgtttgt gtcaacgctg gatggaagtt tgcatgctgt cagcaagagg
     2461 acaggctcaa tcaaatggac tttaaaagaa gatccagtcc tgcaggtccc aacacatgtg
     2521 gaagagcctg cctttctccc agatcctaat gatggcagcc tgtatacgct tggaagcaag
     2581 aataatgaag gcctgacgaa acttcctttt accatcccag aattggtgca ggcatcccca
     2641 tgccgaagtt cagatggaat cctctacatg ggtaaaaagc aggacatctg gtatgttatt
     2701 gacctcctga ccggagagaa gcagcagact ttgtcatcgg cctttgcaga tagtctctgc
     2761 ccatcaacct ctcttctgta tcttgggcga acagaataca ccatcaccat gtacgacacc
     2821 aaaacccgag agctccggtg gaatgccacc tactttgact atgcggcctc actgcctgag
     2881 gacgacgtgg actacaagat gtcccacttt gtgtccaatg gtgatgggct ggtggtgact
     2941 gtggacagtg aatctgggga cgtcctgtgg atccaaaact acgcctcccc tgtggtggcc
     3001 ttttatgtct ggcagcggga gggtctgagg aaggtgatgc acatcaatgt cgctgtggag
     3061 accctgcgct atctgacctt catgtctggg gaggtggggc gcatcacaaa gtggaagtac
     3121 ccgttcccca aggagacaga ggccaagagc aagctgacgc ccactctgta tgttgggaaa
     3181 tactctacca gcctctatgc ctctccctca atggtacacg agggggttgc tgtcgtgccc
     3241 cgcggcagca cacttccttt gctggaaggg ccccagactg atggcgtcac cattggggac
     3301 aagggggagt gtgtgatcac gcccagcacg gacgtcaagt ttgatcccgg actcaaaagc
     3361 aagaacaagc tcaactactt gaggaattac tggcttctga taggacacca tgaaacccca
     3421 ctgtctgcgt ctaccaagat gctggagaga tttcccaaca atctacccaa acatcgggaa
     3481 aatgtgattc ctgctgattc agagaaaaag agctttgagg aagttatcaa cctggttgac
     3541 cagacttcag aaaacgcacc taccaccgtg tctcgggatg tggaggagaa gcccgcccat
     3601 gcccctgccc ggcccgaggc ccccgtggac tccatgctta aggacatggc taccatcatc
     3661 ctgagcacct tcctgctgat tggctgggtg gccttcatca tcacctatcc cctgagcatg
     3721 catcagcagc agcagctcca gcaccagcag ttccagaagg aactggagaa gatccagctc
     3781 ctgcagcagc agcagcagca gctgcccttc cacccacctg gagacacggc tcaggacggc
     3841 gagctcctgg acacgtctgg cccgtactca gagagctcgg gcaccagcag ccccagcacg
     3901 tcccccaggg cctccaacca ctcgctctgc tccggcagct ctgcctccaa ggctggcagc
     3961 agcccctccc tggaacaaga cgatggagat gaggaaacca gcgtggtgat agttgggaaa
     4021 atttccttct gtcccaagga tgtcctgggc catggagctg agggcacaat tgtgtaccgg
     4081 ggcatgtttg acaaccgcga cgtggccgtg aagaggatcc tccccgagtg ttttagcttc
     4141 gcagaccgtg aggtccagct gttgcgagaa tcggatgagc acccgaacgt gatccgctac
     4201 ttctgcacgg agaaggaccg gcaattccag tacattgcca tcgagctgtg tgcagccacc
     4261 ctgcaagagt atgtggagca gaaggacttt gcgcatctcg gcctggagcc catcaccttg
     4321 ctgcagcaga ccacctcggg cctggcccac ctccactccc tcaacatcgt tcacagagac
     4381 ctaaagccac acaacatcct catatccatg cccaatgcac acggcaagat caaggccatg
     4441 atctccgact ttggcctctg caagaagctg gcagtgggca gacacagttt cagccgccga
     4501 tctggggtgc ctggcacaga aggctggatc gctccagaga tgctgagcga agactgtaag
     4561 gagaacccta cctacacggt ggacatcttt tctgcaggct gcgtctttta ctacgtaatc
     4621 tctgagggca gccacccttt tggcaagtcc ctgcagcggc aggccaacat cctcctgggt
     4681 gcctgcagcc ttgactgctt gcacccagag aagcacgaag acgtcattgc acgtgaattg
     4741 atagagaaga tgattgcgat ggatcctcag aaacgcccct cagcgaagca tgtgctcaaa
     4801 cacccgttct tctggagcct agagaagcag ctccagttct tccaggacgt gagcgacaga
     4861 atagaaaagg aatccctgga tggcccgatc gtgaagcagt tagagagagg cgggagagcc
     4921 gtggtgaaga tggactggcg ggagaacatc actgtccccc tccagacaga cctgcgtaaa
     4981 ttcaggacct ataaaggtgg ttctgtcaga gatctcctcc gagccatgag aaataagaag
     5041 caccactacc gggagctgcc tgcagaggtg cgggagacgc tggggtccct ccccgacgac
     5101 ttcgtgtgct acttcacatc tcgcttcccc cacctcctcg cacacaccta ccgggccatg
     5161 gagctgtgca gccacgagag actcttccag ccctactact tccacgagcc cccagagccc
     5221 cagcccccag tgactccaga cgccctcgga tccgactaca aagaccatga cggtgattat
     5281 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     5341 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5401 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5461 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5521 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5581 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5641 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5701 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5761 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     5821 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5881 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     5941 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     6001 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     6061 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     6121 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     6181 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     6241 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     6301 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     6361 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     6421 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     6481 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     6541 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     6601 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     6661 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     6721 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6781 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6841 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6901 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6961 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     7021 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     7081 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     7141 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7201 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     7261 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     7321 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7381 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7441 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7501 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7561 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7621 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7681 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7741 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7801 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7861 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     7921 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7981 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     8041 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8101 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8161 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8221 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     8281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     8401 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8461 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8521 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     8581 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8641 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8701 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8761 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8821 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8881 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8941 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9001 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9061 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9121 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9181 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9241 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9301 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9361 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9421 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9481 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9541 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9601 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9661 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9721 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9781 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9841 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9901 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9961 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10021 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10081 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10141 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10201 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10261 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10321 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10381 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10441 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10501 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10561 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10621 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10681 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10741 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10801 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10861 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10921 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10981 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    11041 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    11101 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    11161 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    11221 g
//
