LOCUS       V035609                11191 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035609
VERSION     V035609
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5251
                     /label="EPHA3(NM_005233)"
                     /note="EPHA3(NM_005233)"
                     /gene="EPHA3"
     CDS             5258..5284
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5288..5314
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5318..5344
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5389..5600
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5613..5881
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5916..6623
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6633..6686
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6687..7286
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7287..7874
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7948..8181
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8253..8374
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8393..8528
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8566..8582)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8590..8606
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8614..8644)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8659..8680
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8968..9556)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9727..10587)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10588..10692)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11166..11182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggattg tcagctctcc atcctcctcc ttctcagctg
     2341 ctctgttctc gacagcttcg gggaactgat tccgcagcct tccaatgaag tcaatctact
     2401 ggattcaaaa acaattcaag gggagctggg ctggatctct tatccatcac atgggtggga
     2461 agagatcagt ggtgtggatg aacattacac acccatcagg acttaccagg tgtgcaatgt
     2521 catggaccac agtcaaaaca attggctgag aacaaactgg gtccccagga actcagctca
     2581 gaagatttat gtggagctca agttcactct acgagactgc aatagcattc cattggtttt
     2641 aggaacttgc aaggagacat tcaacctgta ctacatggag tctgatgatg atcatggggt
     2701 gaaatttcga gagcatcagt ttacaaagat tgacaccatt gcagctgatg aaagtttcac
     2761 tcaaatggat cttggggacc gtattctgaa gctcaacact gagattagag aagtaggtcc
     2821 tgtcaacaag aagggatttt atttggcatt tcaagatgtt ggtgcttgtg ttgccttggt
     2881 gtctgtgaga gtatacttca aaaagtgccc atttacagtg aagaatctgg ctatgtttcc
     2941 agacacggta cccatggact cccagtccct ggtggaggtt agagggtctt gtgtcaacaa
     3001 ttctaaggag gaagatcctc caaggatgta ctgcagtaca gaaggcgaat ggcttgtacc
     3061 cattggcaag tgttcctgca atgctggcta tgaagaaaga ggttttatgt gccaagcttg
     3121 tcgaccaggt ttctacaagg cattggatgg taatatgaag tgtgctaagt gcccgcctca
     3181 cagttctact caggaagatg gttcaatgaa ctgcaggtgt gagaataatt acttccgggc
     3241 agacaaagac cctccatcca tggcttgtac ccgacctcca tcttcaccaa gaaatgttat
     3301 ctctaatata aacgagacct cagttatcct ggactggagt tggcccctgg acacaggagg
     3361 ccggaaagat gttaccttca acatcatatg taaaaaatgt gggtggaata taaaacagtg
     3421 tgagccatgc agcccaaatg tccgcttcct ccctcgacag tttggactca ccaacaccac
     3481 ggtgacagtg acagaccttc tggcacatac taactacacc tttgagattg atgccgttaa
     3541 tggggtgtca gagctgagct ccccaccaag acagtttgct gcggtcagca tcacaactaa
     3601 tcaggctgct ccatcacctg tcctgacgat taagaaagat cggacctcca gaaatagcat
     3661 ctctttgtcc tggcaagaac ctgaacatcc taatgggatc atattggact acgaggtcaa
     3721 atactatgaa aagcaggaac aagaaacaag ttataccatt ctgagggcaa gaggcacaaa
     3781 tgttaccatc agtagcctca agcctgacac tatatacgta ttccaaatcc gagcccgaac
     3841 agccgctgga tatgggacga acagccgcaa gtttgagttt gaaactagtc cagactcttt
     3901 ctccatctct ggtgaaagta gccaagtggt catgatcgcc atttcagcgg cagtagcaat
     3961 tattctcctc actgttgtca tctatgtttt gattgggagg ttctgtggct ataagtcaaa
     4021 acatggggca gatgaaaaaa gacttcattt tggcaatggg catttaaaac ttccaggtct
     4081 caggacttat gttgacccac atacatatga agaccctacc caagctgttc atgagtttgc
     4141 caaggaattg gatgccacca acatatccat tgataaagtt gttggagcag gtgaatttgg
     4201 agaggtgtgc agtggtcgct taaaacttcc ttcaaaaaaa gagatttcag tggccattaa
     4261 gaccctgaaa gttggctaca cagaaaagca gaggagagac ttcctgggag aagcaagcat
     4321 tatgggacag tttgaccacc ccaatatcat tcgactggaa ggagttgtta ccaaaagtaa
     4381 gccagttatg attgtcacag aatacatgga gaatggttcc ttggatagtt tcctacgtaa
     4441 acacgatgcc cagtttactg tcattcagct agtggggatg cttcgaggga tagcatctgg
     4501 catgaagtac ctgtcagaca tgggctatgt tcaccgagac ctcgctgctc ggaacatctt
     4561 gatcaacagt aacttggtgt gtaaggtttc tgatttcgga ctttcgcgtg tcctggagga
     4621 tgacccagaa gctgcttata caacaagagg agggaagatc ccaatcaggt ggacatcacc
     4681 agaagctata gcctaccgca agttcacgtc agccagcgat gtatggagtt atgggattgt
     4741 tctctgggag gtgatgtctt atggagagag accatactgg gagatgtcca atcaggatgt
     4801 aattaaagct gtagatgagg gctatcgact gccacccccc atggactgcc cagctgcctt
     4861 gtatcagctg atgctggact gctggcagaa agacaggaac aacagaccca agtttgagca
     4921 gattgttagt attctggaca agcttatccg gaatcccggc agcctgaaga tcatcaccag
     4981 tgcagccgca aggccatcaa accttcttct ggaccaaagc aatgtggata tcactacctt
     5041 ccgcacaaca ggtgactggc ttaatggtgt ctggacagca cactgcaagg aaatcttcac
     5101 gggtgtggag tacagttctt gtgacacaat agccaagatt tccacagatg acatgaaaaa
     5161 ggttggtgtc accgtggttg ggccacagaa gaagatcatc agtagcatta aagctctaga
     5221 aacgcaatca aagaatggcc cagttcccgt gggatcctac ccatacgacg tacctgatta
     5281 tgcaacttat ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta
     5341 cgcataggcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     5401 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     5461 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     5521 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     5581 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     5641 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     5701 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     5761 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     5821 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     5881 ccaagctgtg accggcgcct acgctagacg ccaccatggt gagcaagggc gaggaggata
     5941 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     6001 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     6061 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     6121 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     6181 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     6241 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     6301 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     6361 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     6421 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     6481 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     6541 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     6601 gcggcatgga cgagctgtac aagggatctc gcgagggcag aggaagtctt ctaacatgcg
     6661 gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca
     6721 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6781 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6841 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6901 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6961 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7021 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     7081 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7141 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7201 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7261 gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat
     7321 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7381 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7441 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7501 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7561 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7621 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7681 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7741 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7801 cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt
     7861 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     7921 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag
     7981 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8041 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8101 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8161 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8221 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8401 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8461 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8521 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc
     8581 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8641 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8701 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8761 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8821 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8881 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8941 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9001 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9061 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9121 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9181 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9241 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9301 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9361 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     9421 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9481 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9541 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9601 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9661 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9721 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9781 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9841 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9901 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9961 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10021 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10081 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10141 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10201 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10261 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10321 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10381 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10441 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10501 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10561 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10621 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10681 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    10741 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    10801 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10861 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10921 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10981 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    11041 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    11101 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    11161 acgttgtaaa acgacggcca gtgccaagct g
//
