LOCUS       V026149                11329 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026149
VERSION     V026149
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5355
                     /label="ELAPOR1(NM_020775)"
                     /note="ELAPOR1(NM_020775)"
                     /gene="ELAPOR1"
     CDS             5362..5427
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5471..5682
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5695..5963
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5992..6001
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6022..6684
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6754..6807
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6808..7407
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7408..7996
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8070..8303
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8375..8496
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8536..8671
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8704..8720)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8728..8744
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8752..8782)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8797..8818
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9106..9694)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9865..10725)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10726..10830)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11304..11320
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctgagcctgg gcacagccac
     2341 catctctccg ccagagtcag gggaagaact gagaggcgca taccccggct gtggcggctg
     2401 ctgctctggg ctgggaccgc cttccaggtg acccagggaa cgggaccgga gcttcatgcc
     2461 tgcaaagagt ctgagtacca ctatgagtac acggcgtgtg acagcacggg ttccaggtgg
     2521 agggtcgccg tgccgcatac cccgggcctg tgcaccagcc tgcctgaccc catcaagggc
     2581 accgagtgct ccttctcctg caacgccggg gagtttctgg atatgaagga ccagtcatgt
     2641 aagccatgcg ctgagggccg ctactccctc ggcacaggca ttcggtttga tgagtgggat
     2701 gagctgcccc atggctttgc cagcctctca gccaacatgg agctggatga cagtgctgct
     2761 gagtccaccg ggaactgtac ttcgtccaag tgggttcccc ggggcgacta catcgcctcc
     2821 aacacggacg aatgcacagc cacactgatg tacgccgtca acctgaagca atctggcacc
     2881 gttaacttcg aatactacta tccagactcc agcatcatct ttgagttttt cgttcagaat
     2941 gaccagtgcc agcccaatgc agatgactcc aggtggatga agaccacaga gaaaggatgg
     3001 gaattccaca gtgtggagct aaatcgaggc aataatgtcc tctattggag aaccacagcc
     3061 ttctcagtat ggaccaaagt acccaagcct gtgctggtga gaaacattgc cataacaggg
     3121 gtggcctaca cttcagaatg cttcccctgc aaacctggca cgtatgcaga caagcagggc
     3181 tcctctttct gcaaactttg cccagccaac tcttattcaa ataaaggaga aacttcttgc
     3241 caccagtgtg accctgacaa atactcagag aaaggatctt cttcctgtaa cgtgcgccca
     3301 gcttgcacag acaaagatta tttctacaca cacacggcct gcgatgccaa cggagagaca
     3361 caactcatgt acaaatgggc caagccgaaa atctgtagcg aggaccttga gggggcagtg
     3421 aagctgcctg cctctggtgt gaagacccac tgcccaccct gcaacccagg cttcttcaaa
     3481 accaacaaca gcacctgcca gccctgccca tatggttcct actccaatgg ctcagactgt
     3541 acccgctgcc ctgcagggac tgaacctgct gtgggatttg aatacaaatg gtggaacacg
     3601 ctgcccacaa acatggaaac gaccgttctc agtgggatca acttcgagta caagggcatg
     3661 acaggctggg aggtggctgg tgatcacatt tacacagctg ctggagcctc agacaatgac
     3721 ttcatgattc tcactctggt tgtgccagga tttagacctc cgcagtcggt gatggcagac
     3781 acagagaata aagaggtggc cagaatcaca tttgtctttg agaccctctg ttctgtgaac
     3841 tgtgagctct acttcatggt gggtgtgaat tctaggacca acactcctgt ggagacgtgg
     3901 aaaggttcca aaggcaaaca gtcctatacc tacatcattg aggagaacac taccacgagc
     3961 ttcacctggg ccttccagag gaccactttt catgaggcaa gcaggaagta caccaatgac
     4021 gttgccaaga tctactccat caatgtcacc aatgttatga atggtgtggc ctcctactgc
     4081 cgtccctgtg ccctagaagc ctctgatgtg ggctcctcct gcacctcttg tcctgctggt
     4141 tactatattg accgagattc aggaacctgc cactcctgcc ccactaacac aattctgaaa
     4201 gcccaccagc cttatggtgt ccaggcctgt gtgccctgtg gtccagggac caagaacaac
     4261 aagatccact ctctgtgcta caacgattgc accttctcac gcaacactcc gaccaggact
     4321 ttcaactaca acttctccgc tttggcaaac actgtcactc ttgctggagg gccaagcttc
     4381 acttccaaag ggctgaaata cttccatcac tttaccctca gtctctgtgg aaaccagggt
     4441 aggaaaatgt ctgtgtgcac cgacaatgtc actgacctcc ggattcctga gggtgagtca
     4501 gggttctcca aatctatcac agcctacgtc tgccaggcag tcatcatccc cccagaggtg
     4561 acaggctaca aggccggggt ttcctcacag cctgtcagcc ttgctgatcg acttattggg
     4621 gtgacaacag atatgactct ggatggaatc acctccccag ctgaactttt ccacctggag
     4681 tccttgggaa taccggacgt gatcttcttt tataggtcca atgatgtgac ccagtcctgc
     4741 agttctggga gatcaaccac catccgcgtc aggtgcagtc cacagaaaac tgtccctgga
     4801 agtttgctgc tgccaggaac gtgctcggat gggacctgtg atggctgcaa cttccacttc
     4861 ctgtgggaga gcgcggctgc ttgcccgctc tgctcagtgg ctgactacca tgctatcgtc
     4921 agcagctgtg tggctgggat ccagaagact acttacgtgt ggcgagaacc caagctatgc
     4981 tctggtggca tttctctgcc tgagcagaga gtcaccatct gcaaaaccat agatttctgg
     5041 ctgaaagtgg gcatctctgc aggcacctgt actgccatcc tgctcaccgt cttgacctgc
     5101 tacttttgga aaaagaatca aaaactagag tacaagtact ccaagctggt gatgaatgct
     5161 actctcaagg actgtgacct gccagcagct gacagctgcg ccatcatgga aggcgaggat
     5221 gtagaggacg acctcatctt taccagcaag aagtcactct ttgggaagat caaatcattt
     5281 acctccaaga ggactcctga tggatttgac tcagtgccgc tgaagacatc ctcaggaggc
     5341 ctagacatgg acctgggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     5401 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     5461 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5521 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5581 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5641 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     5701 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     5761 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     5821 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     5881 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     5941 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     6001 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     6061 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     6121 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     6181 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     6241 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     6301 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     6361 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     6421 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     6481 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     6541 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     6601 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     6661 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     6721 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     6781 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     6841 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6901 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6961 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7021 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7081 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7141 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7201 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7261 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7321 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7381 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     7441 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7501 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7561 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7621 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7681 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7741 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7801 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7861 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7921 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7981 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     8041 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     8101 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8161 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8221 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8281 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8341 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8401 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8461 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8521 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8581 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8641 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
     8701 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8761 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8821 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8881 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8941 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9001 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9301 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9541 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9841 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9901 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9961 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10021 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10081 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10141 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10201 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10261 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10321 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10381 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10441 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10501 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10561 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10621 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10681 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10741 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10801 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10861 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10921 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    10981 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    11041 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    11101 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11161 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11221 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11281 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
