LOCUS       V032627                10795 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032627
VERSION     V032627
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5649
                     /label="EIF2AK3(NM_004836)"
                     /note="EIF2AK3(NM_004836)"
                     /gene="EIF2AK3"
     CDS             5656..5721
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5765..5976
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5989..6257
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6284..6883
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6890..7478
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7552..7785
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7857..7978
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7997..8132
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8170..8186)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8194..8210
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8218..8248)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8263..8284
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8572..9160)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9331..10191)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10192..10296)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10770..10786
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagcgc gccatcagcc cggggctgct ggtacgggcg
     2341 ctgctgctgc tgctgctgct gctggggctc gcggcaagga cggtggccgc ggggcgcgcc
     2401 cgtggcctcc cagcgccgac ggcggaggcg gcgttcggcc tcggggcggc cgctgctccc
     2461 acctcagcga cgcgagtacc ggcggcgggc gccgtggctg cggccgaggt gactgtggag
     2521 gacgctgagg cgctgccggc agccgcggga gagcaggagc ctcggggtcc ggaaccagac
     2581 gatgagacag agttgcgacc gcgcggcagg tcattagtaa ttatcagcac tttagatggg
     2641 agaattgctg ccttggatcc tgaaaatcat ggtaaaaagc agtgggattt ggatgtggga
     2701 tccggttcct tggtgtcatc cagccttagc aaaccagagg tatttgggaa taagatgatc
     2761 attccttccc tggatggagc cctcttccag tgggaccaag accgtgaaag catggaaaca
     2821 gttcctttca cagttgaatc acttcttgaa tcttcttata aatttggaga tgatgttgtt
     2881 ttggttggag gaaaatctct gactacatat ggactcagtg catatagtgg aaaggtgagg
     2941 tatatctgtt cagctctggg ttgtcgccaa tgggatagtg acgaaatgga acaagaggaa
     3001 gacatcctgc ttctacagcg tacccaaaaa actgttagag ctgtcggacc tcgcagtggc
     3061 aatgagaagt ggaatttcag tgttggccac tttgaacttc ggtatattcc agacatggaa
     3121 acgagagccg gatttattga aagcaccttt aagcccaatg agaacacaga agagtctaaa
     3181 attatttcag atgtggaaga acaggaagct gccataatgg acatagtgat aaaggtttcg
     3241 gttgctgact ggaaagttat ggcattcagt aagaagggag gacatctgga atgggagtac
     3301 cagttttgta ctccaattgc atctgcctgg ttacttaagg atgggaaagt cattcccatc
     3361 agtctttttg atgatacaag ttatacatct aatgatgatg ttttagaaga tgaagaagac
     3421 attgtagaag ctgccagagg agccacagaa aacagtgttt acttgggaat gtatagaggc
     3481 cagctgtatc tgcagtcatc agtcagaatt tcagaaaagt ttccttcaag tcccaaggct
     3541 ttggaatctg tcactaatga aaacgcaatt attcctttac caacaatcaa atggaaaccc
     3601 ttaattcatt ctccttccag aactcctgtc ttggtaggat ctgatgaatt tgacaaatgt
     3661 ctcagtaatg ataagttttc tcatgaagaa tatagtaatg gtgcactttc aatcttgcag
     3721 tatccatatg ataatggtta ttatctacca tactacaaga gggagaggaa caaacgaagc
     3781 acacagatta cagtcagatt cctcgacaac ccacattaca acaagaatat ccgcaaaaag
     3841 gatcctgttc ttcttttaca ctggtggaaa gaaatagttg caacgatttt gttttgtatc
     3901 atagcaacaa cgtttattgt gcgcaggctt ttccatcctc atcctcacag gcaaaggaag
     3961 gagtctgaaa ctcagtgtca aactgaaaat aaatatgatt ctgtaagtgg tgaagccaat
     4021 gacagtagct ggaatgacat aaaaaactct ggatatatat cacgatatct aactgatttt
     4081 gagccaattc aatgcctggg acgtggtggc tttggagttg tttttgaagc taaaaacaaa
     4141 gtagatgact gcaattatgc tatcaagagg atccgtctcc ccaataggga attggctcgg
     4201 gaaaaggtaa tgcgagaagt taaagcctta gccaagcttg aacacccggg cattgttaga
     4261 tatttcaatg cctggctcga agcaccacca gagaagtggc aagaaaagat ggatgaaatt
     4321 tggctgaaag atgaaagcac agactggcca ctcagctctc ctagcccaat ggatgcacca
     4381 tcagttaaaa tacgcagaat ggatcctttc gctacaaaag aacatattga aatcatagct
     4441 ccttcaccac aaagaagcag gtctttttca gtagggattt cctgtgacca gacaagttca
     4501 tctgagagcc agttctcacc actggaattc tcaggaatgg accatgagga catcagtgag
     4561 tcagtggatg cagcatacaa cctccaggac agttgcctta cagactgtga tgtggaagat
     4621 gggactatgg atggcaatga tgaggggcac tcctttgaac tttgtccttc tgaagcttct
     4681 ccttatgtaa ggtcaaggga gagaacctcc tcttcaatag tatttgaaga ttctggctgt
     4741 gataatgctt ccagtaaaga agagccgaaa actaatcgat tgcatattgg caaccattgt
     4801 gctaataaac taactgcttt caagcccacc agtagcaaat cttcttctga agctacattg
     4861 tctatttctc ctccaagacc aaccacttta agtttagatc tcactaaaaa caccacagaa
     4921 aaactccagc ccagttcacc aaaggtgtat ctttacattc aaatgcagct gtgcagaaaa
     4981 gaaaacctca aagactggat gaatggacga tgtaccatag aggagagaga gaggagcgtg
     5041 tgtctgcaca tcttcctgca gatcgcagag gcagtggagt ttcttcacag taaaggactg
     5101 atgcacaggg acctcaagcc atccaacata ttctttacaa tggatgatgt ggtcaaggtt
     5161 ggagactttg ggttagtgac tgcaatggac caggatgagg aagagcagac ggttctgacc
     5221 ccaatgccag cttatgccag acacacagga caagtaggga ccaaactgta tatgagccca
     5281 gagcagattc atggaaacag ctattctcat aaagtggaca tcttttcttt aggcctgatt
     5341 ctatttgaat tgctgtatcc attcagcact cagatggaga gagtcaggac cttaactgat
     5401 gtaagaaatc tcaaatttcc accattattt actcagaaat atccttgtga gtacgtgatg
     5461 gttcaagaca tgctctctcc atcccccatg gaacgacctg aagctataaa catcattgaa
     5521 aatgctgtat ttgaggactt ggactttcca ggaaaaacag tgctcagaca gaggtctcgc
     5581 tccttgagtt catcgggaac aaaacattca agacagtcca acaactccca tagccctttg
     5641 ccaagcaatg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     5701 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5761 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5821 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5881 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5941 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     6001 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     6061 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     6121 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     6181 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     6241 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc
     6301 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     6361 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     6421 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6481 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6541 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6601 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6661 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6721 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6781 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6841 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     6901 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6961 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7021 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7081 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7141 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7201 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7261 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     7321 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7381 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7441 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     7501 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7561 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag
     7621 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7681 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7741 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7801 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7861 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7921 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7981 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8041 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8101 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc
     8161 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8221 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8281 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8341 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8401 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8461 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8521 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8581 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8641 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8701 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8761 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8821 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8881 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8941 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9001 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9061 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9241 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9301 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9361 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9421 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9481 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9541 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9601 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9661 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9721 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9781 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9841 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9901 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9961 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10021 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10081 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10141 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10201 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10261 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10321 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10381 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10441 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10501 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10561 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    10621 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10681 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10741 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
