LOCUS       V023894                11602 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023894
VERSION     V023894
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5644
                     /label="EIF2AK3(NM_004836)"
                     /note="EIF2AK3(NM_004836)"
                     /gene="EIF2AK3"
     CDS             5651..5716
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5761..5972
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5985..6253
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6282..6291
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6312..6974
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7044..7097
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7098..7697
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7698..8285
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8359..8592
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8664..8785
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8804..8939
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8977..8993)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9001..9017
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9025..9055)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9070..9091
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9379..9967)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10138..10998)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10999..11103)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11577..11593
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg agcgcgccat cagcccgggg ctgctggtac gggcgctgct
     2341 gctgctgctg ctgctgctgg ggctcgcggc aaggacggtg gccgcggggc gcgcccgtgg
     2401 cctcccagcg ccgacggcgg aggcggcgtt cggcctcggg gcggccgctg ctcccacctc
     2461 agcgacgcga gtaccggcgg cgggcgccgt ggctgcggcc gaggtgactg tggaggacgc
     2521 tgaggcgctg ccggcagccg cgggagagca ggagcctcgg ggtccggaac cagacgatga
     2581 gacagagttg cgaccgcgcg gcaggtcatt agtaattatc agcactttag atgggagaat
     2641 tgctgccttg gatcctgaaa atcatggtaa aaagcagtgg gatttggatg tgggatccgg
     2701 ttccttggtg tcatccagcc ttagcaaacc agaggtattt gggaataaga tgatcattcc
     2761 ttccctggat ggagccctct tccagtggga ccaagaccgt gaaagcatgg aaacagttcc
     2821 tttcacagtt gaatcacttc ttgaatcttc ttataaattt ggagatgatg ttgttttggt
     2881 tggaggaaaa tctctgacta catatggact cagtgcatat agtggaaagg tgaggtatat
     2941 ctgttcagct ctgggttgtc gccaatggga tagtgacgaa atggaacaag aggaagacat
     3001 cctgcttcta cagcgtaccc aaaaaactgt tagagctgtc ggacctcgca gtggcaatga
     3061 gaagtggaat ttcagtgttg gccactttga acttcggtat attccagaca tggaaacgag
     3121 agccggattt attgaaagca cctttaagcc caatgagaac acagaagagt ctaaaattat
     3181 ttcagatgtg gaagaacagg aagctgccat aatggacata gtgataaagg tttcggttgc
     3241 tgactggaaa gttatggcat tcagtaagaa gggaggacat ctggaatggg agtaccagtt
     3301 ttgtactcca attgcatctg cctggttact taaggatggg aaagtcattc ccatcagtct
     3361 ttttgatgat acaagttata catctaatga tgatgtttta gaagatgaag aagacattgt
     3421 agaagctgcc agaggagcca cagaaaacag tgtttacttg ggaatgtata gaggccagct
     3481 gtatctgcag tcatcagtca gaatttcaga aaagtttcct tcaagtccca aggctttgga
     3541 atctgtcact aatgaaaacg caattattcc tttaccaaca atcaaatgga aacccttaat
     3601 tcattctcct tccagaactc ctgtcttggt aggatctgat gaatttgaca aatgtctcag
     3661 taatgataag ttttctcatg aagaatatag taatggtgca ctttcaatct tgcagtatcc
     3721 atatgataat ggttattatc taccatacta caagagggag aggaacaaac gaagcacaca
     3781 gattacagtc agattcctcg acaacccaca ttacaacaag aatatccgca aaaaggatcc
     3841 tgttcttctt ttacactggt ggaaagaaat agttgcaacg attttgtttt gtatcatagc
     3901 aacaacgttt attgtgcgca ggcttttcca tcctcatcct cacaggcaaa ggaaggagtc
     3961 tgaaactcag tgtcaaactg aaaataaata tgattctgta agtggtgaag ccaatgacag
     4021 tagctggaat gacataaaaa actctggata tatatcacga tatctaactg attttgagcc
     4081 aattcaatgc ctgggacgtg gtggctttgg agttgttttt gaagctaaaa acaaagtaga
     4141 tgactgcaat tatgctatca agaggatccg tctccccaat agggaattgg ctcgggaaaa
     4201 ggtaatgcga gaagttaaag ccttagccaa gcttgaacac ccgggcattg ttagatattt
     4261 caatgcctgg ctcgaagcac caccagagaa gtggcaagaa aagatggatg aaatttggct
     4321 gaaagatgaa agcacagact ggccactcag ctctcctagc ccaatggatg caccatcagt
     4381 taaaatacgc agaatggatc ctttcgctac aaaagaacat attgaaatca tagctccttc
     4441 accacaaaga agcaggtctt tttcagtagg gatttcctgt gaccagacaa gttcatctga
     4501 gagccagttc tcaccactgg aattctcagg aatggaccat gaggacatca gtgagtcagt
     4561 ggatgcagca tacaacctcc aggacagttg ccttacagac tgtgatgtgg aagatgggac
     4621 tatggatggc aatgatgagg ggcactcctt tgaactttgt ccttctgaag cttctcctta
     4681 tgtaaggtca agggagagaa cctcctcttc aatagtattt gaagattctg gctgtgataa
     4741 tgcttccagt aaagaagagc cgaaaactaa tcgattgcat attggcaacc attgtgctaa
     4801 taaactaact gctttcaagc ccaccagtag caaatcttct tctgaagcta cattgtctat
     4861 ttctcctcca agaccaacca ctttaagttt agatctcact aaaaacacca cagaaaaact
     4921 ccagcccagt tcaccaaagg tgtatcttta cattcaaatg cagctgtgca gaaaagaaaa
     4981 cctcaaagac tggatgaatg gacgatgtac catagaggag agagagagga gcgtgtgtct
     5041 gcacatcttc ctgcagatcg cagaggcagt ggagtttctt cacagtaaag gactgatgca
     5101 cagggacctc aagccatcca acatattctt tacaatggat gatgtggtca aggttggaga
     5161 ctttgggtta gtgactgcaa tggaccagga tgaggaagag cagacggttc tgaccccaat
     5221 gccagcttat gccagacaca caggacaagt agggaccaaa ctgtatatga gcccagagca
     5281 gattcatgga aacagctatt ctcataaagt ggacatcttt tctttaggcc tgattctatt
     5341 tgaattgctg tatccattca gcactcagat ggagagagtc aggaccttaa ctgatgtaag
     5401 aaatctcaaa tttccaccat tatttactca gaaatatcct tgtgagtacg tgatggttca
     5461 agacatgctc tctccatccc ccatggaacg acctgaagct ataaacatca ttgaaaatgc
     5521 tgtatttgag gacttggact ttccaggaaa aacagtgctc agacagaggt ctcgctcctt
     5581 gagttcatcg ggaacaaaac attcaagaca gtccaacaac tcccatagcc ctttgccaag
     5641 caatggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     5701 ggatgacgat gacaagtgag cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt
     5761 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     5821 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     5881 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     5941 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     6001 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     6061 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     6121 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     6181 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     6241 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg
     6301 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg
     6361 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga
     6421 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct
     6481 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca
     6541 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc
     6601 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg
     6661 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca
     6721 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct
     6781 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga
     6841 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg
     6901 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc
     6961 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg
     7021 gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg
     7081 aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     7141 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     7201 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     7261 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     7321 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     7381 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     7441 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7501 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7561 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7621 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7681 gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7741 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7801 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7861 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7921 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7981 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8041 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     8101 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8161 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8221 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc
     8281 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8341 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt
     8401 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8461 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8521 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8581 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8641 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8701 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8761 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8821 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8881 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8941 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     9001 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9061 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9781 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11101 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    11161 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    11221 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    11281 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    11341 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    11401 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    11461 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    11521 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    11581 aacgacggcc agtgccaagc tg
//
