LOCUS       V029757                11989 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029757
VERSION     V029757
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6015
                     /label="EHMT2(NM_001289413)"
                     /note="EHMT2(NM_001289413)"
                     /gene="EHMT2"
     CDS             6022..6087
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6131..6342
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6355..6623
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6652..6661
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6682..7344
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7414..7467
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7468..8067
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8068..8656
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8730..8963
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9035..9156
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9196..9331
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9364..9380)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9388..9404
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9412..9442)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9457..9478
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9766..10354)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10525..11385)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11386..11490)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11964..11980
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ggggtctacc gagagggagg
     2341 gggttgatgc gggcccgggg gaggggtcgt gcggcccctc cgggcagccg aggccgcgga
     2401 aggggggggc cccacagagg aagaggtagg ccccggagcc tactctctct tcccagggcc
     2461 caggcatcct ggacccccca actctctact gggctgacca gccctcctgt cccttgtctc
     2521 ccctcccagg gggaggcccc cgctgagatg ggggcgctgc tgctggagaa ggaaaccaga
     2581 ggagccaccg agagagttca tggctctttg ggggacaccc ctcgtagtga agaaaccctg
     2641 cccaaggcca cccccgactc cctggagcct gctggcccct catctccagc ctctgtcact
     2701 gtcactgttg gtgatgaggg ggctgacacc cctgtagggg ctacaccact cattggggat
     2761 gaatctgaga atcttgaggg agatggggac ctccgtgggg gccggatcct gctgggccat
     2821 gccacaaagt cattcccctc ttcccccagc aaggggggtt cctgtcctag ccgggccaag
     2881 atgtcaatga caggggcggg aaaatcacct ccatctgtcc agagtttggc tatgaggcta
     2941 ctgagtatgc caggagccca gggagctgca gcagcagggt ctgaaccccc tccagccacc
     3001 acgagcccag agggacagcc caaggtccac cgagcccgca aaaccatgtc caaaccagga
     3061 aatggacagc ccccggtccc tgagaagcgg ccccctgaaa tacagcattt ccgcatgagt
     3121 gatgatgtcc actcactggg aaaggtgacc tcagatctgg ccaaaaggag gaagctgaac
     3181 tcaggaggtg gcctgtcaga ggagttaggt tctgcccggc gttcaggaga agtgaccctg
     3241 acgaaagggg accccgggtc cctggaggag tgggagacgg tggtgggtga tgacttcagt
     3301 ctctactatg attcctactc tgtggatgag cgcgtggact ccgacagcaa gtctgaagtt
     3361 gaagctctaa ctgaacaact aagtgaagag gaggaggagg aagaggagga agaagaagaa
     3421 gaggaagagg aggaggaaga ggaagaagaa gaggaagatg aggagtcagg gaatcagtca
     3481 gataggagtg gttccagtgg ccggcgcaag gccaagaaga aatggcgaaa agacagccca
     3541 tgggtgaagc cgtctcggaa acggcgcaag cgggagcctc cgcgggccaa ggagccacga
     3601 ggggtgtcca atgacacatc ttcgctggag acagagcgag ggtttgagga gttgcccctg
     3661 tgcagctgcc gcatggaggc acccaagatt gaccgcatca gcgagagggc ggggcacaag
     3721 tgcatggcca ctgagagtgt ggacggagag ctgtcaggct gcaatgccgc catcctcaag
     3781 cgggagacca tgaggccatc cagccgtgtg gccctgatgg tgctctgtga gacccaccgc
     3841 gcccgcatgg tcaaacacca ctgctgcccg ggctgcggct acttctgcac ggcgggcacc
     3901 ttcctggagt gccaccctga cttccgtgtg gcccaccgct tccacaaggc ctgtgtgtct
     3961 cagctgaatg ggatggtctt ctgtccccac tgtggggagg atgcttctga agctcaagag
     4021 gtgaccatcc cccggggtga cggggtgacc ccaccggccg gcactgcagc tcctgcaccc
     4081 ccacccctgt cccaggatgt ccccgggaga gcagacactt ctcagcccag tgcccggatg
     4141 cgagggcatg gggaaccccg gcgcccgccc tgcgatcccc tggctgacac cattgacagc
     4201 tcagggccct ccctgaccct gcccaatggg ggctgccttt cagccgtggg gctgccactg
     4261 gggccaggcc gggaggccct ggaaaaggcc ctggtcatcc aggagtcaga gaggcggaag
     4321 aagctccgtt tccaccctcg gcagttgtac ctgtccgtga agcagggcga gctgcagaag
     4381 gtgatcctga tgctgttgga caacctggac cccaacttcc agagcgacca gcagagcaag
     4441 cgcacgcccc tgcatgcagc cgcccagaag ggctccgtgg agatctgcca tgtgctgctg
     4501 caggctggag ccaacataaa tgcagtggac aaacagcagc ggacgccact gatggaggcc
     4561 gtggtgaaca accacctgga ggtagcccgt tacatggtgc agcgtggtgg ctgtgtctat
     4621 agcaaggagg aggacggttc cacctgcctc caccacgcag ccaaaatcgg gaacttggag
     4681 atggtcagcc tgctgctgag cacaggacag gtggacgtca acgcccagga cagtgggggg
     4741 tggacgccca tcatctgggc tgcagagcac aagcacatcg aggtgatccg catgctactg
     4801 acgcggggcg ccgacgtcac cctcactgac aacgaggaga acatctgcct gcactgggcc
     4861 tccttcacgg gcagcgccgc catcgccgaa gtccttctga atgcgcgctg tgacctccat
     4921 gctgtcaact accatgggga cacccccctg cacatcgcag ctcgggagag ctaccatgac
     4981 tgcgtgctgt tattcctgtc acgtggggcc aaccctgagc tgcggaacaa agagggggac
     5041 acagcatggg acctgactcc cgagcgctcc gacgtgtggt ttgcgcttca actcaaccgc
     5101 aagctccgac ttggggtggg aaatcgggcc atccgcacag agaagatcat ctgccgggac
     5161 gtggctcggg gctatgagaa cgtgcccatt ccctgtgtca acggtgtgga tggggagccc
     5221 tgccctgagg attacaagta catctcagag aactgcgaga cgtccaccat gaacatcgat
     5281 cgcaacatca cccacctgca gcactgcacg tgtgtggacg actgctctag ctccaactgc
     5341 ctgtgcggcc agctcagcat ccggtgctgg tatgacaagg atgggcgatt gctccaggaa
     5401 tttaacaaga ttgagcctcc gctgattttc gagtgtaacc aggcgtgctc atgctggaga
     5461 aactgcaaga accgggtcgt acagagtggc atcaaggtgc ggctacagct ctaccgaaca
     5521 gccaagatgg gctggggggt ccgcgccctg cagaccatcc cacaggggac cttcatctgc
     5581 gagtatgtcg gggagctgat ctctgatgct gaggctgatg tgagagagga tgattcttac
     5641 ctcttcgact tagacaacaa ggatggagag gtgtactgca tagatgcccg ttactatggc
     5701 aacatcagcc gcttcatcaa ccacctgtgt gaccccaaca tcattcccgt ccgggtcttc
     5761 atgctgcacc aagacctgcg atttccacgc atcgccttct tcagttcccg agacatccgg
     5821 actggggagg agctagggtt tgactatggc gaccgcttct gggacatcaa aagcaaatat
     5881 ttcacctgcc aatgtggctc tgagaagtgc aagcactcag ccgaagccat tgccctggag
     5941 cagagccgtc tggcccgcct ggacccacac cctgagctgc tgcccgagct cggctccctg
     6001 ccccctgtca acacaggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     6061 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     6121 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6181 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6241 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6301 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     6361 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     6421 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     6481 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     6541 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6601 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     6661 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     6721 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     6781 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     6841 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     6901 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     6961 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     7021 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     7081 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     7141 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     7201 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     7261 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     7321 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     7381 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     7441 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     7501 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7561 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7621 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7681 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7741 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7801 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7861 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7921 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7981 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8041 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     8101 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8161 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8221 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8281 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8341 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8401 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8461 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8521 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8581 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8641 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     8701 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     8761 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8821 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8881 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8941 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     9001 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     9061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9121 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     9181 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9301 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
     9361 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9421 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9481 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9541 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9601 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9661 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9781 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9841 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9901 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9961 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10021 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10081 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10141 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10201 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10261 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10321 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10381 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10441 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10501 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10561 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10621 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10681 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10741 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10801 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10861 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10921 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10981 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11041 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11101 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11161 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11221 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11281 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11341 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11401 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11461 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    11521 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    11581 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    11641 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    11701 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    11761 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11821 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11881 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11941 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
