LOCUS V039989 11830 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039989 VERSION V039989 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3018 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 3055..6684 /EC_number="" /codon_start=1 /gene="" /note="" /product="precursor of human epidermal growth factor receptor" /protein_id="" /transl_table=1 /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKE YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" /label="EGFR" CDS 6691..6756 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 6800..7011 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 7024..7292 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 7319..7918 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7925..8513 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8587..8820 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8892..9013 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 9032..9167 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(9205..9221) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9229..9245 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9253..9283) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9298..9319 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9607..10195) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10366..11226) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11227..11331) /gene="bla" /label="AmpR promoter" primer_bind 11805..11821 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta 2281 gagctagcga attcagccac catggtgagc aagggcgagg agctgttcac cggggtggtg 2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 3001 atggacgagc tgtacaaggg aggaggggga agcggtggag gcggttctct cgagatgcga 3061 ccctccggga cggccggggc agcgctcctg gcgctgctgg ctgcgctctg cccggcgagt 3121 cgggctctgg aggaaaagaa agtttgccaa ggcacgagta acaagctcac gcagttgggc 3181 acttttgaag atcattttct cagcctccag aggatgttca ataactgtga ggtggtcctt 3241 gggaatttgg aaattaccta tgtgcagagg aattatgatc tttccttctt aaagaccatc 3301 caggaggtgg ctggttatgt cctcattgcc ctcaacacag tggagcgaat tcctttggaa 3361 aacctgcaga tcatcagagg aaatatgtac tacgaaaatt cctatgcctt agcagtctta 3421 tctaactatg atgcaaataa aaccggactg aaggagctgc ccatgagaaa tttacaggaa 3481 atcctgcatg gcgccgtgcg gttcagcaac aaccctgccc tgtgcaacgt ggagagcatc 3541 cagtggcggg acatagtcag cagtgacttt ctcagcaaca tgtcgatgga cttccagaac 3601 cacctgggca gctgccaaaa gtgtgatcca agctgtccca atgggagctg ctggggtgca 3661 ggagaggaga actgccagaa actgaccaaa atcatctgtg cccagcagtg ctccgggcgc 3721 tgccgtggca agtcccccag tgactgctgc cacaaccagt gtgctgcagg ctgcacaggc 3781 ccccgggaga gcgactgcct ggtctgccgc aaattccgag acgaagccac gtgcaaggac 3841 acctgccccc cactcatgct ctacaacccc accacgtacc agatggatgt gaaccccgag 3901 ggcaaataca gctttggtgc cacctgcgtg aagaagtgtc cccgtaatta tgtggtgaca 3961 gatcacggct cgtgcgtccg agcctgtggg gccgacagct atgagatgga ggaagacggc 4021 gtccgcaagt gtaagaagtg cgaagggcct tgccgcaaag tgtgtaacgg aataggtatt 4081 ggtgaattta aagactcact ctccataaat gctacgaata ttaaacactt caaaaactgc 4141 acctccatca gtggcgatct ccacatcctg ccggtggcat ttaggggtga ctccttcaca 4201 catactcctc ctctggatcc acaggaactg gatattctga aaaccgtaaa ggaaatcaca 4261 gggtttttgc tgattcaggc ttggcctgaa aacaggacgg acctccatgc ctttgagaac 4321 ctagaaatca tacgcggcag gaccaagcaa catggtcagt tttctcttgc agtcgtcagc 4381 ctgaacataa catccttggg attacgctcc ctcaaggaga taagtgatgg agatgtgata 4441 atttcaggaa acaaaaattt gtgctatgca aatacaataa actggaaaaa actgtttggg 4501 acctccggtc agaaaaccaa aattataagc aacagaggtg aaaacagctg caaggccaca 4561 ggccaggtct gccatgcctt gtgctccccc gagggctgct ggggcccgga gcccagggac 4621 tgcgtctctt gccggaatgt cagccgaggc agggaatgcg tggacaagtg caaccttctg 4681 gagggtgagc caagggagtt tgtggagaac tctgagtgca tacagtgcca cccagagtgc 4741 ctgcctcagg ccatgaacat cacctgcaca ggacggggac cagacaactg tatccagtgt 4801 gcccactaca ttgacggccc ccactgcgtc aagacctgcc cggcaggagt catgggagaa 4861 aacaacaccc tggtctggaa gtacgcagac gccggccatg tgtgccacct gtgccatcca 4921 aactgcacct acggatgcac tgggccaggt cttgaaggct gtccaacgaa tgggcctaag 4981 atcccgtcca tcgccactgg gatggtgggg gccctcctct tgctgctggt ggtggccctg 5041 gggatcggcc tcttcatgcg aaggcgccac atcgttcgga agcgcacgct gcggaggctg 5101 ctgcaggaga gggagcttgt ggagcctctt acacccagtg gagaagctcc caaccaagct 5161 ctcttgagga tcttgaagga aactgaattc aaaaagatca aagtgctggg ctccggtgcg 5221 ttcggcacgg tgtataaggg actctggatc ccagaaggtg agaaagttaa aattcccgtc 5281 gctatcaagg aattaagaga agcaacatct ccgaaagcca acaaggaaat cctcgatgaa 5341 gcctacgtga tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg catctgcctc 5401 acctccaccg tgcagctcat cacgcagctc atgcccttcg gctgcctcct ggactatgtc 5461 cgggaacaca aagacaatat tggctcccag tacctgctca actggtgtgt gcagatcgca 5521 aagggcatga actacttgga ggaccgtcgc ttggtgcacc gcgacctggc agccaggaac 5581 gtactggtga aaacaccgca gcatgtcaag atcacagatt ttgggctggc caaactgctg 5641 ggtgcggaag agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa gtggatggca 5701 ttggaatcaa ttttacacag aatctatacc caccagagtg atgtctggag ctacggggtg 5761 actgtttggg agttgatgac ctttggatcc aagccatatg acggaatccc tgccagcgag 5821 atctcctcca tcctggagaa aggagaacgc ctccctcagc cacccatatg taccatcgat 5881 gtctacatga tcatggtcaa gtgctggatg atagacgcag atagtcgccc aaagttccgt 5941 gagttgatca tcgaattctc caaaatggcc cgagaccccc agcgctacct tgtcattcag 6001 ggggatgaaa gaatgcattt gccaagtcct acagactcca acttctaccg tgccctgatg 6061 gatgaagaag acatggacga cgtggtggat gccgacgagt acctcatccc acagcagggc 6121 ttcttcagca gcccctccac gtcacggact cccctcctga gctctctgag tgcaaccagc 6181 aacaattcca ccgtggcttg cattgataga aatgggctgc aaagctgtcc catcaaggaa 6241 gacagcttct tgcagcgata cagctcagac cccacaggcg ccttgactga ggacagcata 6301 gacgacacct tcctcccagt gcctgaatac ataaaccagt ccgttcccaa aaggcccgct 6361 ggctctgtgc agaatcctgt ctatcacaat cagcctctga accccgcgcc cagcagagac 6421 ccacactacc aggaccccca cagcactgca gtgggcaacc ccgagtatct caacactgtc 6481 cagcccacct gtgtcaacag cacattcgac agccctgccc actgggccca gaaaggcagc 6541 caccaaatta gcctggacaa ccctgactac cagcaggact tctttcccaa ggaagccaag 6601 ccaaatggca tctttaaggg ctccacagct gaaaatgcag aatacctaag ggtcgcgcca 6661 caaagcagtg aatttattgg agcaggatcc gactacaaag accatgacgg tgattataaa 6721 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat 6781 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 6841 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 6901 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 6961 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 7021 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 7081 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 7141 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 7201 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 7261 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat 7321 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg 7381 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg 7441 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 7501 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 7561 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 7621 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 7681 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 7741 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 7801 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 7861 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt 7921 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 7981 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 8041 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 8101 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 8161 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 8221 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 8281 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 8341 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 8401 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 8461 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac 8521 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 8581 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg 8641 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 8701 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 8761 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 8821 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 8881 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 8941 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 9001 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 9061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 9121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 9181 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 9241 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 9301 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 9361 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 9421 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 9481 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 9541 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 9601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 9661 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 9721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 9781 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 9841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 9901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 9961 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 10021 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 10081 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 10141 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 10201 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 10261 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 10321 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 10381 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 10441 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 10501 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 10561 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 10621 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 10681 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 10741 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 10801 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 10861 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 10921 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 10981 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 11041 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 11101 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 11161 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 11221 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 11281 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 11341 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 11401 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 11461 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 11521 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 11581 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 11641 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 11701 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 11761 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 11821 tgccaagctg //