LOCUS       V020004                10873 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020004
VERSION     V020004
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..5798
                     /label="EGF(NM_001178130)"
                     /note="EGF(NM_001178130)"
                     /gene="EGF"
     promoter        5843..6054
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6067..6335
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6362..6961
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6968..7556
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7630..7863
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7935..8056
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8075..8210
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8248..8264)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8272..8288
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8296..8326)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8341..8362
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8650..9238)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9409..10269)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10270..10374)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10848..10864
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atgctgctca ctcttatcat tctgttgcca gtagtttcaa
     2341 aatttagttt tgttagtctc tcagcaccgc agcactggag ctgtcctgaa ggtactctcg
     2401 caggaaatgg gaattctact tgtgtgggtc ctgcaccctt cttaattttc tcccatggaa
     2461 atagtatctt taggattgac acagaaggaa ccaattatga gcaattggtg gtggatgctg
     2521 gtgtctcagt gatcatggat tttcattata atgagaaaag aatctattgg gtggatttag
     2581 aaagacaact tttgcaaaga gtttttctga atgggtcaag gcaagagaga gtatgtaata
     2641 tagagaaaaa tgtttctgga atggcaataa attggataaa tgaagaagtt atttggtcaa
     2701 atcaacagga aggaatcatt acagtaacag atatgaaagg aaataattcc cacattcttt
     2761 taagtgcttt aaaatatcct gcaaatgtag cagttgatcc agtagaaagg tttatatttt
     2821 ggtcttcaga ggtggctgga agcctttata gagcagatct cgatggtgtg ggagtgaagg
     2881 ctctgttgga gacatcagag aaaataacag ctgtgtcatt ggatgtgctt gataagcggc
     2941 tgttttggat tcagtacaac agagaaggaa gcaattctct tatttgctcc tgtgattatg
     3001 atggaggttc tgtccacatt agtaaacatc caacacagca taatttgttt gcaatgtccc
     3061 tttttggtga ccgtatcttc tattcaacat ggaaaatgaa gacaatttgg atagccaaca
     3121 aacacactgg aaaggacatg gttagaatta acctccattc atcatttgta ccacttggtg
     3181 aactgaaagt agtgcatcca cttgcacaac ccaaggcaga agatgacact tgggagcctg
     3241 agcagaaact ttgcaaattg aggaaaggaa actgcagcag cactgtgtgt gggcaagacc
     3301 tccagtcaca cttgtgcatg tgtgcagagg gatacgccct aagtcgagac cggaagtact
     3361 gtgaagatgt taatgaatgt gctttttgga atcatggctg tactcttggg tgtaaaaaca
     3421 cccctggatc ctattactgc acgtgccctg taggatttgt tctgcttcct gatgggaaac
     3481 gatgtcatca acttgtttcc tgtccacgca atgtgtctga atgcagccat gactgtgttc
     3541 tgacatcaga aggtccctta tgtttctgtc ctgaaggctc agtgcttgag agagatggga
     3601 aaacatgtag cggttgttcc tcacccgata atggtggatg tagccagctc tgcgttcctc
     3661 ttagcccagt atcctgggaa tgtgattgct ttcctgggta tgacctacaa ctggatgaaa
     3721 aaagctgtgc agcttcagga ccacaaccat ttttgctgtt tgccaattct caagatattc
     3781 gacacatgca ttttgatgga acagactatg gaactctgct cagccagcag atgggaatgg
     3841 tttatgccct agatcatgac cctgtggaaa ataagatata ctttgcccat acagccctga
     3901 agtggataga gagagctaat atggatggtt cccagcgaga aaggcttatt gaggaaggag
     3961 tagatgtgcc agaaggtctt gctgtggact ggattggccg tagattctat tggacagaca
     4021 gagggaaatc tctgattgga aggagtgatt taaatgggaa acgttccaaa ataatcacta
     4081 aggagaacat ctctcaacca cgaggaattg ctgttcatcc aatggccaag agattattct
     4141 ggactgatac agggattaat ccacgaattg aaagttcttc cctccaaggc cttggccgtc
     4201 tggttatagc cagctctgat ctaatctggc ccagtggaat aacgattgac ttcttaactg
     4261 acaagttgta ctggtgcgat gccaagcagt ctgtgattga aatggccaat ctggatggtt
     4321 caaaacgccg aagacttacc cagaatgatg taggtcaccc atttgctgta gcagtgtttg
     4381 aggattatgt gtggttctca gattgggcta tgccatcagt aatgagagta aacaagagga
     4441 ctggcaaaga tagagtacgt ctccaaggca gcatgctgaa gccctcatca ctggttgtgg
     4501 ttcatccatt ggcaaaacca ggagcagatc cctgcttata tcaaaacgga ggctgtgaac
     4561 atatttgcaa aaagaggctt ggaactgctt ggtgttcgtg tcgtgaaggt tttatgaaag
     4621 cctcagatgg gaaaacgtgt ctggctctgg atggtcatca gctgttggca ggtggtgaag
     4681 ttgatctaaa gaaccaagta acaccattgg acatcttgtc caagactaga gtgtcagaag
     4741 ataacattac agaatctcaa cacatgctag tggctgaaat catggtgtca gatcaagatg
     4801 actgtgctcc tgtgggatgc agcatgtatg ctcggtgtat ttcagaggga gaggatgcca
     4861 catgtcagtg tttgaaagga tttgctgggg atggaaaact atgttctgat atagatgaat
     4921 gtgagatggg tgtcccagtg tgcccccctg cctcctccaa gtgcatcaac accgaaggtg
     4981 gttatgtctg ccggtgctca gaaggctacc aaggagatgg gattcactgt cttgactcta
     5041 ctccaccccc tcacctcagg gaagatgacc accactattc cgtaagaaat agtgactctg
     5101 aatgtcccct gtcccacgat gggtactgcc tccatgatgg tgtgtgcatg tatattgaag
     5161 cattggacaa gtatgcatgc aactgtgttg ttggctacat cggggagcga tgtcagtacc
     5221 gagacctgaa gtggtgggaa ctgcgccacg ctggccacgg gcagcagcag aaggtcatcg
     5281 tggtggctgt ctgcgtggtg gtgcttgtca tgctgctcct cctgagcctg tggggggccc
     5341 actactacag gactcagaag ctgctatcga aaaacccaaa gaatccttat gaggagtcga
     5401 gcagagatgt gaggagtcgc aggcctgctg acactgagga tgggatgtcc tcttgccctc
     5461 aaccttggtt tgtggttata aaagaacacc aagacctcaa gaatgggggt caaccagtgg
     5521 ctggtgagga tggccaggca gcagatgggt caatgcaacc aacttcatgg aggcaggagc
     5581 cccagttatg tggaatgggc acagagcaag gctgctggat tccagtatcc agtgataagg
     5641 gctcctgtcc ccaggtaatg gagcgaagct ttcatatgcc ctcctatggg acacagaccc
     5701 ttgaaggggg tgtcgagaag ccccattctc tcctatcagc taacccatta tggcaacaaa
     5761 gggccctgga cccaccacac caaatggagc tgactcagtg agcggccgcg aaggatctgc
     5821 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5881 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5941 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6001 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     6061 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     6121 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     6181 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     6241 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     6301 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     6361 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     6421 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     6481 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6541 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6601 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6661 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6721 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6781 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6841 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6901 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6961 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7021 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7081 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7141 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7201 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7261 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7321 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7381 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7441 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7501 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7561 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7621 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     7681 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7741 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7801 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7861 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7921 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7981 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8041 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     8101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     8221 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8281 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8341 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8401 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8461 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8521 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8581 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8641 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8701 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8761 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8821 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8881 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8941 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9001 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9061 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9121 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9181 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9241 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9301 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9361 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9421 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9481 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9541 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9601 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9661 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9721 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9781 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9841 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9901 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9961 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10021 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10081 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10141 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10201 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10261 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10321 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10381 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10441 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10501 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10561 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10621 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10681 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10741 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10801 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10861 cagtgccaag ctg
//
