LOCUS       V046642                10508 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046642
VERSION     V046642
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4384
                     /label="TMPO(NM_003276)"
                     /note="TMPO(NM_003276)"
                     /gene="TMPO"
     CDS             4391..4456
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4500..4711
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4724..4992
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5027..5734
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5744..5797
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5804..6598
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6605..7193
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7265..7498
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7570..7691
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7710..7845
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7883..7899)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7907..7923
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7931..7961)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7976..7997
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8285..8873)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9044..9904)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9905..10009)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10483..10499
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgccgga gttcctggaa gacccctcgg tcctgacaaa
     2341 agacaagttg aagagtgagt tggtcgccaa caatgtgacg ctgccggccg gggagcagcg
     2401 caaagacgtg tacgtccagc tctacctgca gcacctcacg gctcgcaacc ggccgccgct
     2461 ccccgccggc accaacagca aggggccccc ggacttctcc agtgacgaag agcgcgagcc
     2521 caccccggtc ctcggctctg gggccgccgc cgcgggccgg agccgagcag ccgtcggcag
     2581 gaaagccaca aaaaaaactg ataaacccag acaagaagat aaagatgatc tagatgtaac
     2641 agagctcact aatgaagatc ttttggatca gcttgtgaaa tacggagtga atcctggtcc
     2701 tattgtggga acaaccagga agctatatga gaaaaagctt ttgaaactga gggaacaagg
     2761 aacagaatca agatcttcta ctcctctgcc aacaatttct tcttcagcag aaaatacaag
     2821 gcagaatgga agtaatgatt ctgacagata cagtgacaat gaagaaggaa agaagaaaga
     2881 acacaagaaa gtgaagtcca ctagggatat tgttcctttt tctgaacttg gaactactcc
     2941 ctctggtggt ggattttttc agggtatttc ttttcctgaa atctccaccc gtcctccttt
     3001 gggcagtacc gaactacagg cagctaagaa agtacatact tctaagggag acctacctag
     3061 ggagcctctt gttgccacaa acttgcctgg caggggacag ttgcagaagt tagcctctga
     3121 aaggaatttg tttatttcat gcaagtctag ccatgatagg tgtttagaga aaagttcttc
     3181 gtcatcttct cagcctgaac acagtgccat gttggtctct actgcagctt ctccttcact
     3241 gattaaagaa accaccactg gttactataa agacatagta gaaaatattt gcggtagaga
     3301 gaaaagtgga attcaaccat tatgtcctga gaggtcccat atttcagatc aatcgcctct
     3361 ctccagtaaa aggaaagcac tagaagagtc tgagagctca caactaattt ctccgccact
     3421 tgcccaggca atcagagatt atgtcaattc tctgttggtc cagggtgggg taggtagttt
     3481 gcctggaact tctaactcta tgcccccact ggatgtagaa aacatacaga agagaattga
     3541 tcagtctaag tttcaagaaa ctgaattcct gtctcctcca agaaaagtcc ctagactgag
     3601 tgagaagtca gtggaggaaa gggattcagg ttcctttgtg gcatttcaga acatacctgg
     3661 atccgaactg atgtcttctt ttgccaaaac tgttgtctct cattcactca ctaccttagg
     3721 tctagaagtg gctaagcaat cacagcatga taaaatagat gcctcagaac tatcttttcc
     3781 cttccatgaa tctattttaa aagtaattga agaagaatgg cagcaagttg acaggcagct
     3841 gccttcactg gcatgcaaat atccagtttc ttccagggag gcaacacaga tattatcagt
     3901 tccaaaagta gatgatgaaa tcctagggtt tatttctgaa gccactccac taggaggtat
     3961 tcaagcagcc tccactgagt cttgcaatca gcagttggac ttagcactct gtagagcata
     4021 tgaagctgca gcatcagcat tgcagattgc aactcacact gcctttgtag ctaaggctat
     4081 gcaggcagac attagtcaag ctgcacagat tcttagctca gatcctagtc gtacccacca
     4141 agcgcttggg attctgagca aaacatatga tgcagcctca tatatttgtg aagctgcatt
     4201 tgatgaagtg aagatggctg cccataccat gggaaatgcc actgtaggtc gtcgatacct
     4261 ctggctgaag gattgcaaaa ttaatttagc ttctaagaat aagctggctt ccactccctt
     4321 taaaggtgga acattatttg gaggagaagt atgcaaagta attaaaaagc gtggaaataa
     4381 acacggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     4441 ggatgacgat gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg
     4501 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     4561 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     4621 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     4681 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     4741 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     4801 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     4861 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     4921 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     4981 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg tgagcaaggg
     5041 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     5101 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     5161 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     5221 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     5281 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5341 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     5401 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     5461 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     5521 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     5581 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     5641 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     5701 ccactccacc ggcggcatgg acgagctgta caagggatct cgcgagggca gaggaagtct
     5761 tctaacatgc ggtgacgtgg aggagaatcc cggccctgcc accatggctg aacaagatgg
     5821 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5881 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5941 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     6001 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6061 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6121 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6181 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6241 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6301 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6361 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6421 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6481 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6541 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagt
     6601 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6661 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6721 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6781 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6841 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6901 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6961 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7021 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7081 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7141 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcggtacct
     7201 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7261 ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg
     7321 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7381 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7441 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7501 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7561 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7681 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc
     7741 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7801 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag
     7861 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7921 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7981 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8041 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8101 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8161 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8221 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8281 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8341 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8401 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8461 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8521 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8581 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8641 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8701 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8761 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8821 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8881 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8941 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9001 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9061 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9121 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9181 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9241 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9301 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9361 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9421 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9481 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9541 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9601 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9661 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9721 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9781 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9841 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9901 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9961 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10021 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
    10081 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
    10141 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
    10201 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
    10261 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    10321 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
    10381 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    10441 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    10501 ccaagctg
//
