LOCUS       V034216                11072 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034216
VERSION     V034216
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4987
                     /label="SF3B2(NM_006842)"
                     /note="SF3B2(NM_006842)"
                     /gene="SF3B2"
     CDS             4994..5020
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5064..5275
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5288..5556
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5591..6298
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6308..6361
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6368..7162
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7169..7757
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7829..8062
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8134..8255
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8274..8409
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8447..8463)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8471..8487
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8495..8525)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8540..8561
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8849..9437)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9608..10468)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10469..10573)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11047..11063
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcgac ggagcatccc gagcctccca aagcagaatt
     2341 gcagctgccg ccgccgccac ctccaggcca ctatggcgcc tgggctgccc aggagcttca
     2401 ggccaagttg gcagagatcg gagctccgat ccagggtaat cgcgaggagc tggtggagcg
     2461 gctgcagagc tacacccgcc agactggcat cgtgctgaat cggccggttt tgagagggga
     2521 agatggggac aaagccgctc cacctcccat gtcggcacag ctccctggaa ttcccatgcc
     2581 accaccacct ttgggactcc cccctctgca gcctcctccg ccacccccac cacctccacc
     2641 aggccttggc cttggctttc ctatggccca cccaccaaat ttggggcccc cgcctcctct
     2701 ccgtgtgggt gagccagtgg cactgtcaga ggaggagcgg ctgaagttgg ctcagcagca
     2761 ggcggcattg ctgatgcagc aggaggagcg tgccaagcag cagggagatc attcgctgaa
     2821 ggaacatgag ctcttggagc agcagaagcg ggcagctgtg ttactggagc aggaacgaca
     2881 gcaggagatt gccaagatgg gcaccccagt ccctcggccc ccacaagaca tgggccagat
     2941 tggtgtgcgc actcctctgg gtcctcgagt agctgctcca gtgggcccag tgggccccac
     3001 tcctacagtt ttgcccatgg gagcccctgt tccccggcct cgtggtcccc caccgccccc
     3061 tggagatgag aacagagaga tggatgaccc ctctgtgggc cccaagatcc cccaggcttt
     3121 ggagaagatc ctgcagctga aggagagccg ccaggaagag atgaattctc agcaggagga
     3181 agaggaaatg gaaacagatg ctcgctcgtc cctgggccag tcagcgtcag agactgagga
     3241 ggacacagtg tccgtatcta aaaaggagaa aaaccggaag cgtaggaacc gaaagaagaa
     3301 gaaaaagccc cagcgggtgc gaggggtgtc ctctgagagc tctggggacc gggagaaaga
     3361 ctcaacccgg tcccgtggct ctgattcccc agcagctgat gttgagattg agtatgtgac
     3421 tgaagaacct gaaatttacg agcccaactt tatcttcttt aagaggatct ttgaggcttt
     3481 taagctcact gatgatgtga agaaggagaa agagaaggag ccagagaaac ttgacaaact
     3541 ggagaactct gcagccccca agaagaaggg atttgaagag gagcacaagg acagtgatga
     3601 tgacagcagt gatgacgagc aggaaaagaa gccagaagcc cccaagctgt ccaagaagaa
     3661 gttgcgccga atgaaccgct tcactgtggc tgaactcaag cagctggtgg ctcggcccga
     3721 tgtcgtggag atgcacgatg tgacagcgca ggaccctaag ctcttggttc acctcaaggc
     3781 cactcggaac tctgtgcctg tgccacgcca ctggtgtttt aagcgcaaat acctgcaggg
     3841 caaacggggc attgagaagc cccccttcga gctgccagac ttcatcaaac gcacaggcat
     3901 ccaggagatg cgagaggccc tgcaggagaa ggaagaacag aagaccatga agtcaaaaat
     3961 gcgagagaaa gttcggccta agatgggcaa aattgacatc gactaccaga aactgcatga
     4021 tgccttcttc aagtggcaga ccaagccaaa gctgaccatc catggggacc tgtactatga
     4081 ggggaaggag ttcgagacac gactgaagga gaagaagcca ggagatctgt ctgatgagct
     4141 aaggatttcc ttggggatgc cagtaggacc aaatgcccac aaggtccctc ccccatggct
     4201 gattgccatg cagcgatatg gaccaccccc atcgtatccc aacctgaaaa tccctgggct
     4261 gaactcgccc atccctgaga gctgttcctt tgggtaccat gctggtggct ggggcaaacc
     4321 tccagtggat gagactggga aaccgctcta tggggacgtg tttggaacca atgctgctga
     4381 atttcagacc aagactgagg aagaagagat tgatcggacc ccttgggggg aactggaacc
     4441 atctgatgaa gaatcctcag aagaagagga agaggaagaa agtgatgaag acaaaccaga
     4501 tgagacaggc tttattaccc ctgcagacag tggccttatc actcctggag gcttttcatc
     4561 agtgcctgct ggaatggaga cccctgaact cattgagctg aggaagaaga agattgagga
     4621 ggcgatggac ggaagtgaga cacctcagct cttcactgtg ttgccagaga agagaacagc
     4681 cactgttgga ggggccatga tgggatcaac ccacatttat gacatgtcca cggttatgag
     4741 ccggaagggc ccggctcctg agctgcaagg tgtggaagtg gcgctggcgc ctgaagagtt
     4801 ggagctggat cctatggcca tgacccagaa gtatgaggag catgtgcggg agcagcaggc
     4861 tcaagtagag aaggaggact tcagtgacat ggtggctgag cacgctgcca aacagaagca
     4921 aaaaaaacgg aaagctcagc cccaggacag ccgtgggggc agcaagaaat ataaggagtt
     4981 caagtttgga tcctacccat acgacgtacc agattacgct tagcggccgc gaaggatctg
     5041 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     5101 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     5161 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     5221 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     5281 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     5341 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     5401 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     5461 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     5521 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     5581 agacgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     5641 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     5701 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     5761 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     5821 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     5881 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     5941 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     6001 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     6061 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     6121 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     6181 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     6241 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg
     6301 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     6361 tgccaccatg gctgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     6421 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     6481 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     6541 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     6601 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     6661 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6721 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6781 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6841 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6901 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6961 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     7021 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     7081 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7141 ccttcttgac gagttcttct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7201 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7261 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7321 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7381 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7441 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7501 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7561 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7621 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7681 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7741 ttgggccgcc tccccgcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7801 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa
     7861 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7921 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7981 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8041 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8101 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8221 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8281 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8341 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8401 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     8461 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8521 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8581 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8641 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9601 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9661 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9721 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9781 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9841 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9901 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9961 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10021 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10081 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10141 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10201 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10261 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10321 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10381 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10441 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10501 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10561 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    10621 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    10681 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    10741 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    10801 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    10861 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    10921 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    10981 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    11041 gacgttgtaa aacgacggcc agtgccaagc tg
//
