LOCUS       V033101                10623 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033101
VERSION     V033101
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4570
                     /label="MLH1(NM_000249)"
                     /note="MLH1(NM_000249)"
                     /gene="MLH1"
     promoter        4615..4826
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4839..5107
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5142..5849
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5859..5912
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5919..6713
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6720..7308
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7380..7613
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7685..7806
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7825..7960
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7998..8014)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8022..8038
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8046..8076)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8091..8112
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8400..8988)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9159..10019)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10020..10124)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10598..10614
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtcgtt cgtggcaggg gttattcggc ggctggacga
     2341 gacagtggtg aaccgcatcg cggcggggga agttatccag cggccagcta atgctatcaa
     2401 agagatgatt gagaactgtt tagatgcaaa atccacaagt attcaagtga ttgttaaaga
     2461 gggaggcctg aagttgattc agatccaaga caatggcacc gggatcagga aagaagatct
     2521 ggatattgta tgtgaaaggt tcactactag taaactgcag tcctttgagg atttagccag
     2581 tatttctacc tatggctttc gaggtgaggc tttggccagc ataagccatg tggctcatgt
     2641 tactattaca acgaaaacag ctgatggaaa gtgtgcatac agagcaagtt actcagatgg
     2701 aaaactgaaa gcccctccta aaccatgtgc tggcaatcaa gggacccaga tcacggtgga
     2761 ggaccttttt tacaacatag ccacgaggag aaaagcttta aaaaatccaa gtgaagaata
     2821 tgggaaaatt ttggaagttg ttggcaggta ttcagtacac aatgcaggca ttagtttctc
     2881 agttaaaaaa caaggagaga cagtagctga tgttaggaca ctacccaatg cctcaaccgt
     2941 ggacaatatt cgctccatct ttggaaatgc tgttagtcga gaactgatag aaattggatg
     3001 tgaggataaa accctagcct tcaaaatgaa tggttacata tccaatgcaa actactcagt
     3061 gaagaagtgc atcttcttac tcttcatcaa ccatcgtctg gtagaatcaa cttccttgag
     3121 aaaagccata gaaacagtgt atgcagccta tttgcccaaa aacacacacc cattcctgta
     3181 cctcagttta gaaatcagtc cccagaatgt ggatgttaat gtgcacccca caaagcatga
     3241 agttcacttc ctgcacgagg agagcatcct ggagcgggtg cagcagcaca tcgagagcaa
     3301 gctcctgggc tccaattcct ccaggatgta cttcacccag actttgctac caggacttgc
     3361 tggcccctct ggggagatgg ttaaatccac aacaagtctg acctcgtctt ctacttctgg
     3421 aagtagtgat aaggtctatg cccaccagat ggttcgtaca gattcccggg aacagaagct
     3481 tgatgcattt ctgcagcctc tgagcaaacc cctgtccagt cagccccagg ccattgtcac
     3541 agaggataag acagatattt ctagtggcag ggctaggcag caagatgagg agatgcttga
     3601 actcccagcc cctgctgaag tggctgccaa aaatcagagc ttggaggggg atacaacaaa
     3661 ggggacttca gaaatgtcag agaagagagg acctacttcc agcaacccca gaaagagaca
     3721 tcgggaagat tctgatgtgg aaatggtgga agatgattcc cgaaaggaaa tgactgcagc
     3781 ttgtaccccc cggagaagga tcattaacct cactagtgtt ttgagtctcc aggaagaaat
     3841 taatgagcag ggacatgagg ttctccggga gatgttgcat aaccactcct tcgtgggctg
     3901 tgtgaatcct cagtgggcct tggcacagca tcaaaccaag ttataccttc tcaacaccac
     3961 caagcttagt gaagaactgt tctaccagat actcatttat gattttgcca attttggtgt
     4021 tctcaggtta tcggagccag caccgctctt tgaccttgcc atgcttgcct tagatagtcc
     4081 agagagtggc tggacagagg aagatggtcc caaagaagga cttgctgaat acattgttga
     4141 gtttctgaag aagaaggctg agatgcttgc agactatttc tctttggaaa ttgatgagga
     4201 agggaacctg attggattac cccttctgat tgacaactat gtgccccctt tggagggact
     4261 gcctatcttc attcttcgac tagccactga ggtgaattgg gacgaagaaa aggaatgttt
     4321 tgaaagcctc agtaaagaat gcgctatgtt ctattccatc cggaagcagt acatatctga
     4381 ggagtcgacc ctctcaggcc agcagagtga agtgcctggc tccattccaa actcctggaa
     4441 gtggactgtg gaacacattg tctataaagc cttgcgctca cacattctgc ctcctaaaca
     4501 tttcacagaa gatggaaata tcctgcagct tgctaacctg cctgatctat acaaagtctt
     4561 tgagaggtgt taagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag
     4621 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
     4681 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     4741 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     4801 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
     4861 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
     4921 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
     4981 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
     5041 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
     5101 acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggtgagc aagggcgagg
     5161 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     5221 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     5281 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     5341 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     5401 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     5461 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     5521 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     5581 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     5641 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     5701 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     5761 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     5821 ccaccggcgg catggacgag ctgtacaagg gatctcgcga gggcagagga agtcttctaa
     5881 catgcggtga cgtggaggag aatcccggcc ctgccaccat ggctgaacaa gatggattgc
     5941 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6001 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6061 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     6121 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6181 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6241 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6301 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6361 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6421 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6481 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6541 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6601 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6661 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagtcgaca
     6721 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6781 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6841 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6901 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6961 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     7021 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     7081 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     7141 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7201 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7261 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgg tacctttaag
     7321 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7381 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     7441 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7501 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7561 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7621 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7681 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7741 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7801 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct
     7861 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7921 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc
     7981 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8041 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8101 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8161 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8821 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9061 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9121 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9181 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9241 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9301 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9361 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9421 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9481 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9541 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9601 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9661 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9721 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9781 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9841 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9901 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9961 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10021 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10081 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10141 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    10201 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    10261 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    10321 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    10381 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    10441 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    10501 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    10561 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
    10621 ctg
//
