LOCUS V033101 10623 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V033101 VERSION V033101 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..4570 /label="MLH1(NM_000249)" /note="MLH1(NM_000249)" /gene="MLH1" promoter 4615..4826 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4839..5107 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5142..5849 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 5859..5912 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5919..6713 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" misc_feature 6720..7308 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7380..7613 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7685..7806 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7825..7960 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7998..8014) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8022..8038 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8046..8076) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8091..8112 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8400..8988) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9159..10019) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10020..10124) /gene="bla" /label="AmpR promoter" primer_bind 10598..10614 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgtcgtt cgtggcaggg gttattcggc ggctggacga 2341 gacagtggtg aaccgcatcg cggcggggga agttatccag cggccagcta atgctatcaa 2401 agagatgatt gagaactgtt tagatgcaaa atccacaagt attcaagtga ttgttaaaga 2461 gggaggcctg aagttgattc agatccaaga caatggcacc gggatcagga aagaagatct 2521 ggatattgta tgtgaaaggt tcactactag taaactgcag tcctttgagg atttagccag 2581 tatttctacc tatggctttc gaggtgaggc tttggccagc ataagccatg tggctcatgt 2641 tactattaca acgaaaacag ctgatggaaa gtgtgcatac agagcaagtt actcagatgg 2701 aaaactgaaa gcccctccta aaccatgtgc tggcaatcaa gggacccaga tcacggtgga 2761 ggaccttttt tacaacatag ccacgaggag aaaagcttta aaaaatccaa gtgaagaata 2821 tgggaaaatt ttggaagttg ttggcaggta ttcagtacac aatgcaggca ttagtttctc 2881 agttaaaaaa caaggagaga cagtagctga tgttaggaca ctacccaatg cctcaaccgt 2941 ggacaatatt cgctccatct ttggaaatgc tgttagtcga gaactgatag aaattggatg 3001 tgaggataaa accctagcct tcaaaatgaa tggttacata tccaatgcaa actactcagt 3061 gaagaagtgc atcttcttac tcttcatcaa ccatcgtctg gtagaatcaa cttccttgag 3121 aaaagccata gaaacagtgt atgcagccta tttgcccaaa aacacacacc cattcctgta 3181 cctcagttta gaaatcagtc cccagaatgt ggatgttaat gtgcacccca caaagcatga 3241 agttcacttc ctgcacgagg agagcatcct ggagcgggtg cagcagcaca tcgagagcaa 3301 gctcctgggc tccaattcct ccaggatgta cttcacccag actttgctac caggacttgc 3361 tggcccctct ggggagatgg ttaaatccac aacaagtctg acctcgtctt ctacttctgg 3421 aagtagtgat aaggtctatg cccaccagat ggttcgtaca gattcccggg aacagaagct 3481 tgatgcattt ctgcagcctc tgagcaaacc cctgtccagt cagccccagg ccattgtcac 3541 agaggataag acagatattt ctagtggcag ggctaggcag caagatgagg agatgcttga 3601 actcccagcc cctgctgaag tggctgccaa aaatcagagc ttggaggggg atacaacaaa 3661 ggggacttca gaaatgtcag agaagagagg acctacttcc agcaacccca gaaagagaca 3721 tcgggaagat tctgatgtgg aaatggtgga agatgattcc cgaaaggaaa tgactgcagc 3781 ttgtaccccc cggagaagga tcattaacct cactagtgtt ttgagtctcc aggaagaaat 3841 taatgagcag ggacatgagg ttctccggga gatgttgcat aaccactcct tcgtgggctg 3901 tgtgaatcct cagtgggcct tggcacagca tcaaaccaag ttataccttc tcaacaccac 3961 caagcttagt gaagaactgt tctaccagat actcatttat gattttgcca attttggtgt 4021 tctcaggtta tcggagccag caccgctctt tgaccttgcc atgcttgcct tagatagtcc 4081 agagagtggc tggacagagg aagatggtcc caaagaagga cttgctgaat acattgttga 4141 gtttctgaag aagaaggctg agatgcttgc agactatttc tctttggaaa ttgatgagga 4201 agggaacctg attggattac cccttctgat tgacaactat gtgccccctt tggagggact 4261 gcctatcttc attcttcgac tagccactga ggtgaattgg gacgaagaaa aggaatgttt 4321 tgaaagcctc agtaaagaat gcgctatgtt ctattccatc cggaagcagt acatatctga 4381 ggagtcgacc ctctcaggcc agcagagtga agtgcctggc tccattccaa actcctggaa 4441 gtggactgtg gaacacattg tctataaagc cttgcgctca cacattctgc ctcctaaaca 4501 tttcacagaa gatggaaata tcctgcagct tgctaacctg cctgatctat acaaagtctt 4561 tgagaggtgt taagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag 4621 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg 4681 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 4741 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 4801 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac 4861 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc 4921 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga 4981 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc 5041 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt 5101 acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggtgagc aagggcgagg 5161 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg 5221 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 5281 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt 5341 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact 5401 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 5461 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg 5521 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg 5581 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca 5641 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca 5701 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca 5761 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact 5821 ccaccggcgg catggacgag ctgtacaagg gatctcgcga gggcagagga agtcttctaa 5881 catgcggtga cgtggaggag aatcccggcc ctgccaccat ggctgaacaa gatggattgc 5941 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 6001 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 6061 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 6121 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 6181 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 6241 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 6301 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 6361 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 6421 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 6481 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 6541 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 6601 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 6661 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagtcgaca 6721 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 6781 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 6841 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 6901 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 6961 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 7021 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 7081 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 7141 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 7201 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 7261 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgg tacctttaag 7321 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 7381 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct 7441 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 7501 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 7561 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 7621 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 7681 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 7741 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 7801 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct 7861 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 7921 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc 7981 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 8041 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 8101 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 8161 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 8221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 8281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 8341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 8401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 8461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 8521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 8581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 8641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 8701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 8761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 8821 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 8881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 8941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 9001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 9061 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 9121 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 9181 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 9241 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 9301 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 9361 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 9421 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 9481 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 9541 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 9601 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 9661 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 9721 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 9781 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 9841 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 9901 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 9961 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 10021 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 10081 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 10141 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 10201 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 10261 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 10321 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 10381 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 10441 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 10501 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 10561 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 10621 ctg //