LOCUS       V030489                10436 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030489
VERSION     V030489
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4312
                     /label="DDX18(NM_006773)"
                     /note="DDX18(NM_006773)"
                     /gene="DDX18"
     CDS             4319..4384
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4428..4639
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4652..4920
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4955..5662
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5672..5725
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5732..6526
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6533..7121
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7193..7426
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7498..7619
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7638..7773
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7811..7827)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7835..7851
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7859..7889)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7904..7925
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8213..8801)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8972..9832)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9833..9937)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10411..10427
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtcaca cctgccgatg aaactcctgc gtaagaagat
     2341 cgagaagcgg aacctcaaat tgcggcagcg gaacctaaag tttcaggggg cctcaaatct
     2401 gaccctatcg gaaactcaaa atggagatgt atctgaagaa acaatgggaa gtagaaaggt
     2461 taaaaaatca aaacaaaagc ccatgaatgt gggcttatca gaaactcaaa atggaggcat
     2521 gtctcaagaa gcagtgggaa atataaaagt tacaaagtct ccccagaaat ccactgtatt
     2581 aaccaatgga gaagcagcaa tgcagtcttc caattcagaa tcaaaaaaga aaaagaagaa
     2641 aaagagaaaa atggtgaatg atgctgagcc tgatacgaaa aaagcaaaaa ctgaaaacaa
     2701 agggaaatct gaagaagaaa gtgccgagac tactaaagaa acagaaaata atgtggagaa
     2761 gccagataat gatgaagatg agagtgaggt gcccagtctg cccctgggac tgacaggagc
     2821 ttttgaggat acttcgtttg cttctctatg taatcttgtc aatgaaaaca ctctgaaggc
     2881 aataaaagaa atgggtttta caaacatgac tgaaattcag cataaaagta tcagaccact
     2941 tctggaaggc agggatcttc tagcagctgc aaaaacaggc agtggtaaaa ccctggcttt
     3001 tctcatccct gcagttgaac tcattgttaa gttaaggttc atgcccagga atggaacagg
     3061 agtccttatt ctctcaccta ctagagaact agccatgcaa acctttggtg ttcttaagga
     3121 gctgatgact caccacgtgc atacctatgg cttgataatg ggtggcagta acagatctgc
     3181 tgaagcacag aaacttggta atgggatcaa catcattgtg gccacaccag gccgtctgct
     3241 ggaccatatg cagaataccc caggatttat gtataaaaac ctgcagtgtc tggttattga
     3301 tgaagctgat cgtatcttgg atgtggggtt tgaagaggaa ttaaagcaaa ttattaaact
     3361 tttgccaaca cgtagacaga ctatgctctt ttctgccacc caaactcgaa aagttgaaga
     3421 cctggcaagg atttctctga aaaaggagcc attgtatgtt ggcgttgatg atgataaagc
     3481 gaatgcaaca gtggatggtc ttgaacaggg atatgttgtt tgtccttctg aaaagagatt
     3541 ccttctgctc tttacattcc ttaagaagaa ccgaaagaag aagcttatgg tcttcttttc
     3601 atcttgtatg tctgtgaaat accactatga gttgctgaac tacattgatt tgcccgtctt
     3661 ggccattcat ggaaagcaaa agcaaaataa gcgtacaacc acattcttcc agttctgcaa
     3721 tgcagattcg ggaacactat tgtgtacgga tgtggcagcg agaggactag acattcctga
     3781 agtcgactgg attgttcagt atgaccctcc ggatgaccct aaggaatata ttcatcgtgt
     3841 gggtagaaca gccagaggcc taaatgggag agggcatgcc ttgctcattt tgcgcccaga
     3901 agaattgggt tttcttcgct acttgaaaca atccaaggtt ccattaagtg aatttgactt
     3961 ttcctggtct aaaatttctg acattcagtc tcagcttgag aaattgattg aaaagaatta
     4021 ctttcttcat aagtcagccc aggaagcata taagtcatac atacgagcct atgattccca
     4081 ttctctgaaa cagatcttta atgttaataa cctaaatttg cctcaggttg ctctgtcatt
     4141 tggtttcaag gtgcctccct tcgttgatct gaacgtcaac agtaatgaag gcaagcagaa
     4201 aaagcgagga ggtggtggtg gatttggcta ccagaaaacc aagaaagttg agaaatccaa
     4261 aatctttaaa cacattagca agaaatcatc tgacagcagg cagttctctc acggatccga
     4321 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     4381 caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     4441 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     4501 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     4561 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     4621 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     4681 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     4741 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     4801 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     4861 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     4921 caagctgtga ccggcgccta cgctagacgc caccatggtg agcaagggcg aggaggataa
     4981 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     5041 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     5101 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     5161 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     5221 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     5281 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     5341 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     5401 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     5461 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     5521 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     5581 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     5641 cggcatggac gagctgtaca agggatctcg cgagggcaga ggaagtcttc taacatgcgg
     5701 tgacgtggag gagaatcccg gccctgccac catggctgaa caagatggat tgcacgcagg
     5761 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5821 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5881 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     5941 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6001 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6061 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6121 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6181 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6241 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6301 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6361 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6421 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6481 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagtcg acaatcaacc
     6541 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6601 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6661 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6721 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6781 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6841 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6901 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6961 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7021 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7081 ccctcagacg agtcggatct ccctttgggc cgcctccccg cggtaccttt aagaccaatg
     7141 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7201 ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta
     7261 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7321 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7381 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7441 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7501 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7561 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7621 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7681 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7741 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt
     7801 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7861 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7921 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7981 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8041 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8101 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8161 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8221 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8281 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8341 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8401 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8461 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8521 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8581 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8641 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8701 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8761 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8821 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8881 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8941 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9001 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9061 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9121 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9181 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9241 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9301 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9361 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9421 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9481 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9541 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9601 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9661 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9721 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9781 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9841 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9901 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9961 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    10021 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    10081 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10141 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    10201 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10261 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    10321 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    10381 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
