LOCUS       V036911                10822 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036911
VERSION     V036911
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5475
                     /label="UBA1(NM_003334)"
                     /note="UBA1(NM_003334)"
                     /gene="UBA1"
     CDS             5482..5547
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5591..5802
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5815..6083
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6116..6910
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6917..7505
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7579..7812
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7884..8005
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8024..8159
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8197..8213)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8221..8237
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8245..8275)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8290..8311
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8599..9187)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9358..10218)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10219..10323)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10797..10813
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtccagc tcgccgctgt ccaagaaacg tcgcgtgtcc
     2341 gggcctgatc caaagccggg ttctaactgc tcccctgccc agtccgtgtt gtccgaagtg
     2401 ccctcggtgc caaccaacgg aatggccaag aacggcagtg aagcagacat agacgagggc
     2461 ctttactccc ggcagctgta tgtgttgggc catgaggcaa tgaagcggct ccagacatcc
     2521 agtgtcctgg tatcaggcct gcggggcctg ggcgtggaga tcgctaagaa catcatcctt
     2581 ggtggggtca aggctgttac cctacatgac cagggcactg cccagtgggc tgatctttcc
     2641 tcccagttct acctgcggga ggaggacatc ggtaaaaacc gggccgaggt atcacagccc
     2701 cgcctcgctg agctcaacag ctatgtgcct gtcactgcct acactggacc cctcgttgag
     2761 gacttcctta gtggtttcca ggtggtggtg ctcaccaaca cccccctgga ggaccagctg
     2821 cgagtgggtg agttctgtca caaccgtggc atcaagctgg tggtggcaga cacgcggggc
     2881 ctgtttgggc agctcttctg tgactttgga gaggaaatga tcctcacaga ttccaatggg
     2941 gagcagccac tcagtgctat ggtttctatg gttaccaagg acaaccccgg tgtggttacc
     3001 tgcctggatg aggcccgaca cgggtttgag agcggggact ttgtctcctt ttcagaagta
     3061 cagggcatgg ttgaactcaa cggaaatcag cccatggaga tcaaagtcct gggtccttat
     3121 acctttagca tctgtgacac ctccaacttc tccgactaca tccgtggagg catcgtcagt
     3181 caggtcaaag tacctaagaa gattagcttt aaatccttgg tggcctcact ggcagaacct
     3241 gactttgtgg tgacggactt cgccaagttt tctcgccctg cccagctgca cattggcttc
     3301 caggccctgc accagttctg tgctcagcat ggccggccac ctcggccccg caatgaggag
     3361 gatgcagcag aactggtagc cttagcacag gctgtgaatg ctcgagccct gccagcagtg
     3421 cagcaaaata acctggacga ggacctcatc cggaagctgg catatgtggc tgctggggat
     3481 ctggcaccca taaacgcctt cattgggggc ctggctgccc aggaagtcat gaaggcctgc
     3541 tccgggaagt tcatgcccat catgcagtgg ctatactttg atgcccttga gtgtctccct
     3601 gaggacaaag aggtcctcac agaggacaag tgcctccagc gccagaaccg ttatgacggg
     3661 caagtggctg tgtttggctc agacctgcaa gagaagctgg gcaagcagaa gtatttcctg
     3721 gtgggtgcgg gggccattgg ctgtgagctg ctcaagaact ttgccatgat tgggctgggc
     3781 tgcggggagg gtggagaaat catcgttaca gacatggaca ccattgagaa gtcaaatctg
     3841 aatcgacagt ttcttttccg gccctgggat gtcacgaagt taaagtctga cacggctgct
     3901 gcagctgtgc gccaaatgaa tccacatatc cgggtgacaa gccaccagaa ccgtgtgggt
     3961 cctgacacgg agcgcatcta tgatgacgat tttttccaaa acctagatgg cgtggccaat
     4021 gccctggaca acgtggatgc ccgcatgtac atggaccgcc gctgtgtcta ctaccggaag
     4081 ccactgctgg agtcaggcac actgggcacc aaaggcaatg tgcaggtggt gatccccttc
     4141 ctgacagagt cgtacagttc cagccaggac ccacctgaga agtccatccc catctgtacc
     4201 ctgaagaact tccctaatgc catcgagcac accctgcagt gggctcggga tgagtttgaa
     4261 ggcctcttca agcagccagc agaaaatgtc aaccagtacc tcacagaccc caagtttgtg
     4321 gagcgaacac tgcggctggc aggcactcag cccttggagg tgctggaggc tgtgcagcgc
     4381 agcctggtgc tgcagcgacc acagacctgg gctgactgcg tgacctgggc ctgccaccac
     4441 tggcacaccc agtactcgaa caacatccgg cagctgctgc acaacttccc tcctgaccag
     4501 ctcacaagct caggagcgcc gttctggtct gggcccaaac gctgtccaca cccgctcacc
     4561 tttgatgtca acaatcccct gcatctggac tatgtgatgg ctgctgccaa cctgtttgcc
     4621 cagacctacg ggctgacagg ctctcaggac cgagctgctg tggccacatt cctgcagtct
     4681 gtgcaggtcc ccgaattcac ccccaagtct ggcgtcaaga tccatgtttc tgaccaggag
     4741 ctgcagagcg ccaatgcctc tgttgatgac agtcgtctag aggagctcaa agccactctg
     4801 cccagcccag acaagctccc tggattcaag atgtacccca ttgactttga gaaggatgat
     4861 gacagcaact ttcatatgga tttcatcgtg gctgcatcca acctccgggc agaaaactat
     4921 gacattcctt ctgcagaccg gcacaagagc aagctgattg cagggaagat catcccagcc
     4981 attgccacga ccacagcagc cgtggttggc cttgtgtgtc tggagctgta caaggttgtg
     5041 caggggcacc gacagcttga ctcctacaag aatggtttcc tcaacttggc cctgcctttc
     5101 tttggtttct ctgaacccct tgccgcacca cgtcaccagt actataacca agagtggaca
     5161 ttgtgggatc gctttgaggt acaagggctg cagcctaatg gtgaggagat gaccctcaaa
     5221 cagttcctcg actattttaa gacagagcac aaattagaga tcaccatgct gtcccagggc
     5281 gtgtccatgc tctattcctt cttcatgcca gctgccaagc tcaaggaacg gttggatcag
     5341 ccgatgacag agattgtgag ccgtgtgtcg aagcgaaagc tgggccgcca cgtgcgggcg
     5401 ctggtgcttg agctgtgctg taacgacgag agcggcgagg atgtcgaggt tccctatgtc
     5461 cgatacacca tccgcggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     5521 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     5581 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5641 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5701 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5761 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     5821 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     5881 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     5941 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     6001 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6061 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctagg ccaccatggc
     6121 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6181 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6241 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6301 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6361 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6421 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6481 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6541 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6601 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6661 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6721 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6781 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6841 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6901 gttcttctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6961 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7021 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7081 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7141 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7201 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7261 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7321 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7381 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7441 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7501 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7561 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt
     7621 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7681 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7741 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7801 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7861 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     7921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7981 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8041 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8101 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8161 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     8221 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8281 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8461 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8761 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9001 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9241 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9301 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9361 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9421 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9481 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9541 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9601 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9661 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9721 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9781 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9841 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9901 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9961 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10021 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10081 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10141 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10201 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10261 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10321 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10381 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10441 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10501 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10561 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10621 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10681 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10741 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10801 aacgacggcc agtgccaagc tg
//
