LOCUS       V039149                 9886 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039149
VERSION     V039149
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4518
                     /label="SUZ12(NM_015355)"
                     /note="SUZ12(NM_015355)"
                     /gene="SUZ12"
     CDS             4525..4551
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4555..4581
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4585..4611
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4655..4866
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4879..5147
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5180..5974
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    5981..6569
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6643..6876
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6948..7069
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7088..7223
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7261..7277)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7285..7301
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7309..7339)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7354..7375
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7663..8251)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8422..9282)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9283..9387)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9861..9877
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcgcct cagaagcacg gcggtggggg agggggcggc
     2341 tcggggccca gcgcggggtc cgggggaggc ggcttcgggg gttcggcggc ggtggcggcg
     2401 gcgacggctt cgggcggcaa atccggcggc gggagctgtg gagggggtgg cagttactcg
     2461 gcctcctcct cctcctccgc ggcggcagcg gcgggggctg cggtgttacc ggtgaagaag
     2521 ccgaaaatgg agcacgtcca ggctgaccac gagcttttcc tccaggcctt tgagaagcca
     2581 acacagatct atagatttct tcgaactcgg aatctcatag caccaatatt tttgcacaga
     2641 actcttactt acatgtctca tcgaaactcc agaacaaaca tcaaaaggaa aacatttaaa
     2701 gttgatgata tgttatcaaa agtagagaaa atgaaaggag agcaagaatc tcatagcttg
     2761 tcagctcatt tgcagcttac gtttactggt ttcttccaca aaaatgataa gccatcacca
     2821 aactcagaaa atgaacaaaa ttctgttacc ctggaagtcc tgcttgtgaa agtttgccac
     2881 aaaaaaagaa aggatgtaag ttgtccaata aggcaagttc ccacaggtaa aaagcaggtg
     2941 cctttgaatc ctgacctcaa tcaaacaaaa cccggaaatt tcccgtccct tgcagtttcc
     3001 agtaatgaat ttgaacctag taacagccat atggtgaagt cttactcgtt gctatttaga
     3061 gtgactcgtc caggaagaag agagtttaat ggaatgatta atggagaaac caatgaaaat
     3121 attgatgtca atgaagagct tccagccaga agaaaacgaa atcgtgagga tggggaaaag
     3181 acatttgttg cacaaatgac agtatttgat aaaaacaggc gcttacagct tttagatggg
     3241 gaatatgaag tagccatgca ggaaatggaa gaatgtccaa taagcaagaa aagagcaaca
     3301 tgggagacta ttcttgatgg gaagaggctg cctccattcg aaacattttc tcagggacct
     3361 acgttgcagt tcactcttcg ttggacagga gagaccaatg ataaatctac ggctcctatt
     3421 gccaaacctc ttgccactag aaattcagag agtctccatc aggaaaacaa gcctggttca
     3481 gttaaaccta ctcaaactat tgctgttaaa gaatcattga ctacagatct acaaacaaga
     3541 aaagaaaagg atactccaaa tgaaaaccga caaaaattaa gaatatttta tcagtttctc
     3601 tataacaaca atacaaggca acaaactgaa gcaagagatg acctgcattg cccttggtgt
     3661 actctgaact gccgcaaact ttatagttta ctcaagcatc ttaaactctg ccatagcaga
     3721 tttatcttca actatgttta tcatccaaaa ggtgctagga tagatgtttc tatcaatgag
     3781 tgttatgatg gctcctatgc aggaaatcct caggatattc atcgccaacc tggatttgct
     3841 tttagtcgca acggaccagt taagagaaca cctatcacac atattcttgt gtgcaggcca
     3901 aaacgaacaa aagcaagcat gtctgaattt cttgaatctg aagatgggga agtagaacag
     3961 caaagaacat atagtagtgg ccacaatcgt ctgtatttcc atagtgatac ctgcttacct
     4021 ctccgtccac aagaaatgga agtagatagt gaagatgaaa aggatcctga atggctaaga
     4081 gaaaaaacca ttacacaaat tgaagagttt tctgatgtta atgaaggaga gaaagaagtg
     4141 atgaaactct ggaatctcca tgtcatgaag catgggttta ttgctgacaa tcaaatgaat
     4201 catgcctgta tgctgtttgt agaaaattat ggacagaaaa taattaagaa gaatttatgt
     4261 cgaaacttca tgcttcatct agtcagcatg catgacttta atcttattag cataatgtca
     4321 atagataaag ctgttaccaa gctccgtgaa atgcagcaaa aattagaaaa gggggaatct
     4381 gcttcccctg caaacgaaga aataactgaa gaacaaaatg ggacagcaaa tggatttagt
     4441 gaaattaact caaaagagaa agctttggaa acagatagtg tctcaggggt ttcaaaacag
     4501 agcaaaaaac aaaaactcgg atcctaccca tacgacgtac ctgattatgc aacttatcct
     4561 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc atagcggccg
     4621 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     4681 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     4741 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     4801 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     4861 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     4921 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     4981 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5041 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5101 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5161 gcgcctacgc taggccacca tggctgaaca agatggattg cacgcaggtt ctccggccgc
     5221 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5281 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5341 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5401 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5461 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5521 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5581 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5641 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5701 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5761 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5821 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5881 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5941 cgccttctat cgccttcttg acgagttctt ctgagtcgac aatcaacctc tggattacaa
     6001 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6061 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6121 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6181 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6241 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6301 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6361 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6421 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6481 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6541 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     6601 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6661 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     6901 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     6961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     7081 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7141 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7201 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     7261 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7321 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7381 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7441 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8101 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8941 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9421 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9481 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     9541 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     9601 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     9661 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     9721 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9781 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9841 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
