LOCUS V039149 9886 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039149 VERSION V039149 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4518 /label="SUZ12(NM_015355)" /note="SUZ12(NM_015355)" /gene="SUZ12" CDS 4525..4551 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4555..4581 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4585..4611 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4655..4866 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4879..5147 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5180..5974 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" misc_feature 5981..6569 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6643..6876 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6948..7069 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7088..7223 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7261..7277) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7285..7301 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7309..7339) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7354..7375 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7663..8251) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8422..9282) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9283..9387) /gene="bla" /label="AmpR promoter" primer_bind 9861..9877 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcgcct cagaagcacg gcggtggggg agggggcggc 2341 tcggggccca gcgcggggtc cgggggaggc ggcttcgggg gttcggcggc ggtggcggcg 2401 gcgacggctt cgggcggcaa atccggcggc gggagctgtg gagggggtgg cagttactcg 2461 gcctcctcct cctcctccgc ggcggcagcg gcgggggctg cggtgttacc ggtgaagaag 2521 ccgaaaatgg agcacgtcca ggctgaccac gagcttttcc tccaggcctt tgagaagcca 2581 acacagatct atagatttct tcgaactcgg aatctcatag caccaatatt tttgcacaga 2641 actcttactt acatgtctca tcgaaactcc agaacaaaca tcaaaaggaa aacatttaaa 2701 gttgatgata tgttatcaaa agtagagaaa atgaaaggag agcaagaatc tcatagcttg 2761 tcagctcatt tgcagcttac gtttactggt ttcttccaca aaaatgataa gccatcacca 2821 aactcagaaa atgaacaaaa ttctgttacc ctggaagtcc tgcttgtgaa agtttgccac 2881 aaaaaaagaa aggatgtaag ttgtccaata aggcaagttc ccacaggtaa aaagcaggtg 2941 cctttgaatc ctgacctcaa tcaaacaaaa cccggaaatt tcccgtccct tgcagtttcc 3001 agtaatgaat ttgaacctag taacagccat atggtgaagt cttactcgtt gctatttaga 3061 gtgactcgtc caggaagaag agagtttaat ggaatgatta atggagaaac caatgaaaat 3121 attgatgtca atgaagagct tccagccaga agaaaacgaa atcgtgagga tggggaaaag 3181 acatttgttg cacaaatgac agtatttgat aaaaacaggc gcttacagct tttagatggg 3241 gaatatgaag tagccatgca ggaaatggaa gaatgtccaa taagcaagaa aagagcaaca 3301 tgggagacta ttcttgatgg gaagaggctg cctccattcg aaacattttc tcagggacct 3361 acgttgcagt tcactcttcg ttggacagga gagaccaatg ataaatctac ggctcctatt 3421 gccaaacctc ttgccactag aaattcagag agtctccatc aggaaaacaa gcctggttca 3481 gttaaaccta ctcaaactat tgctgttaaa gaatcattga ctacagatct acaaacaaga 3541 aaagaaaagg atactccaaa tgaaaaccga caaaaattaa gaatatttta tcagtttctc 3601 tataacaaca atacaaggca acaaactgaa gcaagagatg acctgcattg cccttggtgt 3661 actctgaact gccgcaaact ttatagttta ctcaagcatc ttaaactctg ccatagcaga 3721 tttatcttca actatgttta tcatccaaaa ggtgctagga tagatgtttc tatcaatgag 3781 tgttatgatg gctcctatgc aggaaatcct caggatattc atcgccaacc tggatttgct 3841 tttagtcgca acggaccagt taagagaaca cctatcacac atattcttgt gtgcaggcca 3901 aaacgaacaa aagcaagcat gtctgaattt cttgaatctg aagatgggga agtagaacag 3961 caaagaacat atagtagtgg ccacaatcgt ctgtatttcc atagtgatac ctgcttacct 4021 ctccgtccac aagaaatgga agtagatagt gaagatgaaa aggatcctga atggctaaga 4081 gaaaaaacca ttacacaaat tgaagagttt tctgatgtta atgaaggaga gaaagaagtg 4141 atgaaactct ggaatctcca tgtcatgaag catgggttta ttgctgacaa tcaaatgaat 4201 catgcctgta tgctgtttgt agaaaattat ggacagaaaa taattaagaa gaatttatgt 4261 cgaaacttca tgcttcatct agtcagcatg catgacttta atcttattag cataatgtca 4321 atagataaag ctgttaccaa gctccgtgaa atgcagcaaa aattagaaaa gggggaatct 4381 gcttcccctg caaacgaaga aataactgaa gaacaaaatg ggacagcaaa tggatttagt 4441 gaaattaact caaaagagaa agctttggaa acagatagtg tctcaggggt ttcaaaacag 4501 agcaaaaaac aaaaactcgg atcctaccca tacgacgtac ctgattatgc aacttatcct 4561 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc atagcggccg 4621 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 4681 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4741 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 4801 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4861 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4921 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4981 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 5041 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 5101 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 5161 gcgcctacgc taggccacca tggctgaaca agatggattg cacgcaggtt ctccggccgc 5221 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 5281 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 5341 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 5401 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 5461 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 5521 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 5581 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 5641 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5701 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5761 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5821 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5881 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5941 cgccttctat cgccttcttg acgagttctt ctgagtcgac aatcaacctc tggattacaa 6001 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 6061 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 6121 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6181 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6241 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 6301 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 6361 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 6421 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 6481 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 6541 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg 6601 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 6661 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 6721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 6781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 6841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 6901 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 6961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 7021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 7081 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 7141 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 7201 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 7261 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7321 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7381 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7441 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 7621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 7681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 7741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 7801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 8041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 8101 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 8161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 8341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 8461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 8521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 8581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 8641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 8701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 8761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 8821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8941 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 9001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 9061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 9121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 9181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 9301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 9421 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 9481 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 9541 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 9601 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 9661 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 9721 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 9781 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 9841 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg //