LOCUS       V045869                10225 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045869
VERSION     V045869
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4857
                     /label="PIWIL4(NM_152431)"
                     /note="PIWIL4(NM_152431)"
                     /gene="PIWIL4"
     CDS             4864..4890
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4894..4920
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4924..4950
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4994..5205
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5218..5486
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5519..6313
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6320..6908
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6982..7215
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7287..7408
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7427..7562
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7600..7616)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7624..7640
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7648..7678)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7693..7714
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8002..8590)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8761..9621)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9622..9726)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10200..10216
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgagtgga agagcccgag tgaaggccag aggcatcgcc
     2341 cgcagcccca gtgccacaga agtggggcgc atccaagcct cgccattgcc tagatctgtt
     2401 gatcttagta acaatgaagc atcctctagc aatggcttct tgggaacaag caggatctca
     2461 accaacgata aatatgggat atcttctggt gatgctggaa gtaccttcat ggaaagaggt
     2521 gtgaaaaaca aacaggactt tatggatttg agtatctgta ccagagaaaa attggcacat
     2581 gtgagaaatt gtaaaacagg ttccagtgga atacctgtga aactggttac aaacctcttt
     2641 aacttagatt ttccccaaga ctggcagcta taccagtacc atgtgacata tattccagat
     2701 ttagcatcta gaaggctgag aattgcttta ctttatagtc atagtgaact ttccaacaaa
     2761 gcaaaagcat tcgacggtgc catccttttt ctgtcacaaa agctagaaga aaaggtcaca
     2821 gagttgtcaa gtgaaactca aagaggtgag actataaaga tgactatcac cctgaagagg
     2881 gagctgccat caagttctcc cgtgtgcatc caggtcttca atatcatctt cagaaagatc
     2941 ctcaaaaagt tgtccatgta ccaaattgga cggaacttct ataatccttc agagccaatg
     3001 gaaattcccc agcacaaatt atccctttgg cctgggtttg ccatttctgt gtcatatttt
     3061 gaaaggaagc tcctgtttag tgctgatgtg agttacaaag tcctccggaa tgagacggtt
     3121 ctggaattca tgactgctct ctgtcaaaga actggcttgt cctgtttcac ccagacgtgt
     3181 gagaagcagc taatagggct cattgtcctt acaagataca ataacagaac ctactccatt
     3241 gatgacattg actggtcagt gaagcccaca cacacctttc agaagcggga tggcaccgag
     3301 atcacctatg tggattacta caagcagcag tatgatatta ctgtatcgga cctgaatcag
     3361 cccatgcttg ttagtctgtt aaagaagaag agaaatgaca acagtgaggc tcagctcgcc
     3421 cacctgatac ctgagctctg ctttctaaca gggctgactg accaggcaac atctgatttc
     3481 cagctgatga aggctgtggc tgaaaagaca cgtctcagtc cttcaggccg gcagcagcgc
     3541 ctggccaggc ttgtggacaa catccagagg aataccaatg ctcgctttga actagagacc
     3601 tggggactgc attttggaag ccagatatct ctgactggcc ggattgtgcc ttcagaaaaa
     3661 atattaatgc aagaccacat atgtcaacct gtgtctgctg ctgactggtc caaggatatt
     3721 cgaacttgca agattttaaa tgcacagtct ttgaatacct ggttgatttt atgtagcgac
     3781 agaactgaat atgttgccga gagctttctg aactgcttga gaagagttgc aggttccatg
     3841 ggatttaatg tggactaccc caaaatcata aaagtacaag aaaatccagc tgcatttgtt
     3901 agagctatac agcaatatgt tgatcctgat gttcagctgg taatgtgcat tctgccttct
     3961 aatcagaaga cctattatga ttccattaaa aaatatttga gctcagactg cccagtccca
     4021 agccaatgtg tgcttgctcg gaccttgaat aaacagggca tgatgatgag tatcgccacc
     4081 aagatcgcta tgcagatgac ttgcaagctc ggaggcgagc tgtgggctgt ggaaatacct
     4141 ttaaagtccc tgatggtggt cggtattgat gtctgtaaag atgcactcag caaggacgtg
     4201 atggttgttg gatgcgtggc cagtgttaac cccagaatca ccaggtggtt ttcccgctgt
     4261 atccttcaga gaacaatgac tgatgttgca gattgcttga aagttttcat gactggagca
     4321 ctcaacaaat ggtacaagta caatcatgat ttgccagcac ggataattgt gtaccgtgct
     4381 ggtgtagggg atggtcagct gaaaacactt attgaatatg aagtcccaca gctgctgagc
     4441 agtgtggcag aatccagctc aaataccagc tcaagactgt cggtgattgt ggtcaggaag
     4501 aagtgcatgc cacgattctt taccgaaatg aaccgcactg tacagaaccc cccacttggc
     4561 actgttgtgg attcagaagc aacacgtaac gaatggtatg acttttatct gatcagccag
     4621 gtggcctgcc ggggaactgt tagtcctacc tactataatg tcatctatga tgacaacggc
     4681 ttgaagcccg accatatgca gagacttaca ttcaaattgt gccacctgta ctacaactgg
     4741 ccgggcatag tcagtgtccc agcaccatgt cagtatgctc acaagctgac ctttctggtg
     4801 gcacaaagca ttcataaaga acccagtctg gaattagcca accatctctt ctacctggga
     4861 tcctacccat acgacgtacc tgattatgca acttatcctt atgatgtacc tgattatgct
     4921 agctatcctt atgatgtccc agactacgca tagcggccgc gaaggatctg cgatcgctcc
     4981 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5041 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5101 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5161 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5221 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5281 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5341 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5401 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5461 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct aggccaccat
     5521 ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5581 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5641 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     5701 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5761 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5821 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5881 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5941 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6001 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6061 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6121 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6181 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6241 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     6301 cgagttcttc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6361 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6421 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6481 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6541 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6601 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6661 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6721 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6781 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6841 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6901 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6961 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
     7021 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7081 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7141 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7201 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7261 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7321 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7381 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7441 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7501 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7561 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
     7621 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7681 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7741 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7801 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7861 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7921 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7981 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8041 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8101 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8161 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8221 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8281 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8341 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8401 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8461 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8521 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8581 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8641 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8701 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8761 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8821 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8881 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8941 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9001 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9061 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9121 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9181 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9241 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9301 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9361 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9421 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9481 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9541 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9601 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9661 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9721 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     9781 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     9841 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     9901 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     9961 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10021 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    10081 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10141 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10201 taaaacgacg gccagtgcca agctg
//
