LOCUS       V026361                10705 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026361
VERSION     V026361
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5358
                     /label="MCC(NM_001085377)"
                     /note="MCC(NM_001085377)"
                     /gene="MCC"
     CDS             5365..5430
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5474..5685
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5698..5966
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5999..6793
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6800..7388
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7462..7695
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7767..7888
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7907..8042
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8080..8096)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8104..8120
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8128..8158)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8173..8194
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8482..9070)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9241..10101)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10102..10206)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10680..10696
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatggcg gccgcggcgg cagcggctgc ggggagctcc
     2341 agcagcggcg gcggcggcgg cggcagcggc agcagcagca gcagcagcga cacgtccagc
     2401 accggcgagg aggagaggat gcggcgcctc ttccagacgt gcgacggcga cggggacgga
     2461 tacatcagca gaaatgactt gctaatggtc tgtcgccagc tgaatatgga agagtctgtg
     2521 gctgagatca tgaaccagtt gggagcagat gaaaatggga agatttcctt tcaggatttc
     2581 acaagatgcc gcatgcagct tgttcgagaa attaggaagg aggaagtaga tctttctgca
     2641 aagtcagaca actcctgtac aaagaagctg agggatagaa ttgcttcctg gcccacgagc
     2701 agtgacaaca gtttgggtgc cttatcagca gccagggaga gctgggaata cgactctggt
     2761 gccagggacc tccagagccc ggatgtgcag agccagtcag ccctccagaa gctgctcgag
     2821 tatggcggaa gctctttgca tcagcaggct gctctccaca aactgctcac acagtccccg
     2881 cacattggca actctgtagg aggaagctat ctagagctgg ccaacacact ccattcagca
     2941 gccctggcat cactaaaggg agatatagtg gaacttaata aacgtctcca gcaaacagag
     3001 agggaacggg accttctgga aaagaaattg gccaaggcac agtgcgagca gtcccacctc
     3061 atgagagagc atgaggatgt ccaggagcga acgacacttc gctatgagga acgcatcaca
     3121 gagctccaca gcgtcattgc ggagctcaac aagaagatag accgtctgca aggcaccacc
     3181 atcagggagg aagatgagta ctcagaactg cgatcagaac tcagccagag ccaacacgag
     3241 gtcaacgagg actctcgaag catggaccaa gaccagacct ctgtctctat ccccgaaaac
     3301 cagtctacca tggttactgc tgacatggac aactgcagtg acctgaactc agaactgcag
     3361 agggtgctga cagggctgga gaatgttgtc tgcggcagga agaagagcag ctgcagcctc
     3421 tccgtggccg aggtggacaa gcacattgag cagctcacca cagccagcga gcactgtgac
     3481 ctggctatta agacagtcga ggagattgag ggggtgcttg gccgggacct gtatcccaac
     3541 ctggctgaag agaggtctcg gtgggagaag gagctggctg ggctgaggga agagaatgag
     3601 agcctgactg ccatgctgtg cagcaaagag gaagaactga accggactaa ggccaccatg
     3661 aatgccatcc gggaagagcg ggaccggctc cggaggaggg tcagagagct tcaaactcga
     3721 ctacagagcg tgcaggccac aggtccctcc agccctggcc gcctcacttc caccaaccgc
     3781 ccgattaacc ccagcactgg ggagctgagc acaagcagca gcagcaatga cattcccatc
     3841 gccaagattg ctgagagggt gaagctatca aagacaaggt ccgaatcgtc atcatctgat
     3901 cggccagtcc tgggctcaga aatcagtagc ataggggtat ccagcagtgt ggctgaacac
     3961 ctggcccact cacttcagga ctgctccaat atccaagaga ttttccaaac actctactca
     4021 cacggatctg ccatctcaga aagcaagatt agagagtttg aggtggaaac agaacggctg
     4081 aatagccgga ttgagcacct caaatcccaa aatgacctcc tgaccataac cttggaggaa
     4141 tgtaaaagca atgccgagag gatgagcatg ctggtgggaa aatacgaatc caatgccaca
     4201 gcgctgaggc tggccttgca gtacagcgag cagtgcatcg aagcctacga actcctcctg
     4261 gcgctggcag agagtgagca gagcctcatc ctggggcagt tccgagcggc gggcgtgggg
     4321 tcctcccctg gagaccagtc gggggatgaa aacatcactc agatgctcaa gcgagctcat
     4381 gactgccgga agacagctga gaacgctgcc aaggccctgc tcatgaagct ggacggcagc
     4441 tgtgggggag cctttgccgt ggccggctgc agcgtgcagc cctgggagag cctttcctcc
     4501 aacagccaca ccagcacaac cagctccaca gccagtagtt gcgacaccga gttcactaaa
     4561 gaagacgagc agaggctgaa ggattatatc cagcagctca agaatgacag ggctgcggtc
     4621 aagctgacca tgctggagct ggaaagcatc cacatcgatc ctctcagcta tgacgtcaag
     4681 cctcggggag acagccagag gctggatctg gaaaacgcag tgcttatgca ggagctcatg
     4741 gccatgaagg aggagatggc cgagttgaag gcccagctct acctactgga gaaagagaag
     4801 aaggccctgg agctgaagct gagcacgcgg gaggcccagg agcaggccta cctggtgcac
     4861 attgagcacc tgaagtccga ggtggaggag cagaaggagc agcggatgcg atccctcagc
     4921 tccaccagca gcggcagcaa agacaaacct ggcaaggagt gtgctgatgc tgcctcccca
     4981 gctctgtccc tagccgaact caggacaacg tgcagcgaga atgagctggc tgcggagttc
     5041 accaacgcca ttcgtcgaga aaagaagttg aaggccagag ttcaagagct ggtgagtgcc
     5101 ttggagagac tcaccaagag cagtgaaatc cgacatcagc aatctgcaga gttcgtgaat
     5161 gatctaaagc gggccaacag caacctggtg gctgcctatg agaaagcaaa gaaaaagcat
     5221 caaaacaaac tgaagaagtt agagtcgcag atgatggcca tggtggagag acatgagacc
     5281 caagtgagga tgctcaagca aagaatagct ctgctagagg aggagaactc caggccacac
     5341 accaatgaaa cttcgcttct cgaggactac aaagaccatg acggtgatta taaagatcat
     5401 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     5461 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5521 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5581 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5641 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5701 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5761 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5821 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5881 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5941 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct aggccaccat
     6001 ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6061 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6121 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6181 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6241 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6301 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6361 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6421 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6481 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6541 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6601 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6661 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6721 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     6781 cgagttcttc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6841 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6901 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6961 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7021 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7081 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7141 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7201 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7261 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7321 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7381 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7441 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
     7501 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7681 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7741 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7801 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7861 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7921 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7981 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8041 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
     8101 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8161 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8221 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8281 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8881 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10081 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10141 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10201 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10261 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    10321 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10381 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10441 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10501 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    10561 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10621 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10681 taaaacgacg gccagtgcca agctg
//
