LOCUS       V042844                10307 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042844
VERSION     V042844
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             3367..3426
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from equine rhinitis A virus
                     polyprotein"
                     /transl_table=1
                     /translation="QCTNYALLKLAGDVESNPGP"
                     /label="E2A"
     CDS             3427..5025
                     /label="B4galnt1(NM_008080)"
                     /note="B4galnt1(NM_008080)"
                     /gene="B4galnt1"
     promoter        5076..5287
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5300..5568
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5601..6395
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6402..6990
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7064..7297
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7369..7490
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7509..7644
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7682..7698)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7706..7722
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7730..7760)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7775..7796
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8084..8672)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8843..9703)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9704..9808)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10282..10298
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgagcccc tgcgggcggg ccctacatac gtccagaggg
     2341 gccatggcca tgctagctcg gaaattcccg cgtacccggc tacccgtagg agccagtgcc
     2401 ctctgtgtgg tggtcctctg ttggctctac atcttccctg tctaccggct gcccaacgag
     2461 aaggagattg tgcaaggggt gctggcacag cggacggctt ggaggacgaa ccagacgtca
     2521 gccagtctct tcaggagaca aatggaagac tgctgtgacc ctgcccatct ctttgctatg
     2581 actaagatga actcccccat ggggaagagc ctgtggtacg atggggagct cttatactcg
     2641 ttcaccatcg ataattccac gtactccctc ttcccccagg caaccccctt ccagctgcca
     2701 ttgaagaaat gtgcggtggt gggaaacggt gggattctga agatgagtgg ctgtggccgt
     2761 caaatagatg aagcaaattt tgtcatgcgg tgcaacctgc ctcccttgtc aagtgagtac
     2821 accagagatg tcggttccaa aactcagttg gtgacagcta accccagcat aattcgccag
     2881 agatttgaaa acctgctgtg gtccagaaag aagtttgtgg acaacatgaa gatctataac
     2941 cacagttaca tctacatgcc tgccttttct atgaagacag gcacagagcc gtctctccgt
     3001 gtgtattaca cactgaaaga tgttggagcc aatcaaacag tgctttttgc taaccccaac
     3061 tttcttcgga acattggaaa gttctggaag agtaggggga tccacgccaa gcgcttgtct
     3121 acaggactct ttttggtgag tgcagccttg ggcctctgtg aggaggtgtc catctatggc
     3181 ttctggccct tctcggtgaa catgcaaggg gaccctatca gtcaccacta ctatgacaac
     3241 gtcttgccct tctcaggcta ccatgccatg cctgaggaat tccttcagct ttggtatctt
     3301 cacaaaatcg gcgcgctgag gatgcagctg gacccgtgtg aggagccgtc accacagccc
     3361 acttcccagt gcaccaacta cgccctgctg aagctggccg gcgacgtgga gagcaacccc
     3421 ggccccatgc ggctagaccg ccgggccctc tatgcgctag tcttgctgct cgcctgcgcc
     3481 tcgctgggtc tcctgtactc cagcacccga aacgcgccaa gtctcccgaa ccctctggca
     3541 ttgtggtcgc ccccacaagg tcccccgaga ctcgatctgc tagaccttgc ccctgagcct
     3601 cgctacgcac acatcccggt caggatcaag gagcaagtgg tggggctgct ggctcagaac
     3661 aactgcagtt gtgaatccaa gggaggaagc cttcccttgc cctttctgag acaggttcgg
     3721 gcggttgacc tcactaaagc ctttgacgct gaggagctga gggctgtttc tgtcgccagg
     3781 gagcaggaat accaggcctt ccttgcaagg agccggtccc tggctgacca gctgctgata
     3841 gctcccgcca actccccctt acagtacccc ctgcagggtg tggaggttca gcccctcagg
     3901 agcatcctgg tgccagggct aagtctgcag gaagcttctg tccaggagat ataccaggtg
     3961 aacctgagtg cttctctagg cacctgggac gtggcagggg aagtaacagg agtgactctc
     4021 acgggagagg ggcaaccgga tctcaccctt gccagcccag ttctggataa actcaaccgg
     4081 cagctgcaac tggtgactta cagcagccgg agctaccagg ccaacacagc agacacagtc
     4141 cggttctcca ccaagggaca tgaagttgcc ttcaccatcc tcgtaagaca ccctcccaac
     4201 ccccggctgt acccaccatc atccctaccc caaggagccg agtacaacat cagtgctctg
     4261 gttaccattg ccaccaagac ttttcttcgt tatgatcgac tacggacact cattgccagc
     4321 atccgacgct tttaccctac ggtcaccata gtcatcgctg acgacagtga caaaccggag
     4381 cgcattagcg acccacacgt ggagcactac ttcatgccct tcggcaaggg ttggttcgca
     4441 ggtcggaacc tggcggtgtc ccaagtaacc accaaatacg tgctgtgggt ggacgacgac
     4501 tttgtcttca cggcgcgcac gcggctggag aagcttgtgg acgtgctcga gaagacgccc
     4561 ctggacctgg tagggggcgc ggtgcgggag atctcgggct acgctaccac ctaccggcag
     4621 ctgctgagtg tggagccggg cgccccaggc cttgggaact gcttccggca aaagcagggc
     4681 ttccaccacg agctcgttgg cttcccaagt tgtgtggtca cagacggcgt ggtcaacttc
     4741 ttcctggcgc gcacagacaa agtgcgccaa gtgggctttg acccacgcct caaccgggtg
     4801 gcacatctgg aattcttcct ggatggactt ggtttccttc gtgttggctc ctgctctgat
     4861 gttgttgtgg atcatgcatc gaaggtgaag ctgccgtgga cagcaaaaga cccaggagct
     4921 gaaacttatg cccgttacag gtacccggga tcactggacc aaagtcaagt ggccaaacat
     4981 cgcctactct tcttcaaaca ccggctacag tgcatgaccg ccgagtgagg atccgcggcc
     5041 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     5101 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     5161 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     5221 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     5281 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     5341 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     5401 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     5461 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     5521 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     5581 ggcgcctacg ctaggccacc atggctgaac aagatggatt gcacgcaggt tctccggccg
     5641 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     5701 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5761 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     5821 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5881 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5941 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6001 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6061 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6121 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6181 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6241 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6301 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6361 tcgccttcta tcgccttctt gacgagttct tctgagtcga caatcaacct ctggattaca
     6421 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6481 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6541 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6601 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6661 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6721 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6781 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     6841 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6901 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6961 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7021 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7081 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7141 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7201 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7261 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7321 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7381 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7441 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7501 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7621 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     7681 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7741 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7801 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7861 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7921 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7981 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8041 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8101 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8161 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8221 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8281 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8341 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8401 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8461 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8521 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8581 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8641 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8701 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8761 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8821 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8881 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8941 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9001 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9061 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9121 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9181 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9241 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9301 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9361 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9421 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9481 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9541 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9601 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9661 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9721 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9781 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9841 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9901 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9961 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10021 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10081 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10141 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10201 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10261 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
