LOCUS       V031906                12407 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031906
VERSION     V031906
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..6235
                     /label="ZNF521(NM_015461)"
                     /note="ZNF521(NM_015461)"
                     /gene="ZNF521"
     CDS             6251..6316
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6360..6571
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6584..6852
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6881..6890
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6911..7573
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7643..7696
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7703..8497
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    8504..9092
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9164..9397
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9469..9590
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9609..9744
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9782..9798)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9806..9822
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9830..9860)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9875..9896
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10184..10772)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10943..11803)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11804..11908)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12382..12398
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtctcg ccgcaagcaa gcgaaaccga gatccctcaa
     2341 agaccccaac tgtaaacttg aagacaagac tgaagatgga gaggcactag attgtaagaa
     2401 gaggccggaa gacggggagg agttggaaga cgaagctgtg cacagctgtg acagctgcct
     2461 ccaggtgttt gaatcgctga gcgatatcac agaacacaag attaatcaat gtcaactgac
     2521 agatggagtg gatgttgaag atgatccgac ttgctcttgg ccagcttcct caccttctag
     2581 caaggatcag acttccccta gccatggaga aggttgcgat tttggagagg aagaaggtgg
     2641 ccctgggctt ccatacccgt gtcaattctg tgacaagtcg tttagccgcc tcagctacct
     2701 aaagcaccat gagcagagtc acagtgacaa actgcctttc aaatgcacct actgcagtag
     2761 gctgttcaaa cacaagcgca gccgagatcg ccacataaaa ctccacaccg gggacaagaa
     2821 gtaccactgc agtgaatgtg atgctgcgtt ttccagaagt gatcacttga agatccactt
     2881 aaagactcac acgtccaaca agccatataa atgtgccatt tgtcgccgtg ggtttctgtc
     2941 ctctagttcc ttacacggac acatgcaggt tcatgagagg aacaaggacg gctctcagtc
     3001 cggttccagg atggaggact ggaagatgaa ggacactcag aagtgcagtc agtgtgagga
     3061 aggctttgac ttcccggaag acctccaaaa acacattgca gagtgccacc ccgaatgctc
     3121 cccaaatgag gaccgagcgg ccctccagtg tgtctactgc cacgagctct tcgtagagga
     3181 gacctccctc atgaaccaca tggagcaggt gcatagcggg gagaagaaga actcatgcag
     3241 catttgttct gagagtttcc acacagttga ggaactgtac agccacatgg acagtcacca
     3301 gcaaccggag tcatgcaatc acagcaacag cccttccctg gtcacggtgg gctatacctc
     3361 cgtgtccagt acgactccag attccaacct ctcagtggac agctcaacca tggtggaagc
     3421 tgccccgcca atcccaaaga gtcgagggag gaagagggcc gctcaacaaa cccctgacat
     3481 gactggtccc tcgagtaaac aagcaaaagt tacctacagc tgtatttact gcaacaaaca
     3541 attattttca agtcttgcag ttctgcagat tcacctgaaa actatgcact tagataagcc
     3601 agaacaggcc catatttgtc agtattgctt ggaggtcctg ccctcactct ataacctaaa
     3661 tgaacatctt aagcaagtgc atgaagctca ggacccaggt ctgattgttt ctgccatgcc
     3721 tgccattgtc taccagtgta acttctgttc cgaagttgtc aacgacctca acactcttca
     3781 ggaacacatc cgatgttctc atggatttgc aaaccctgca gctaaagata gtaatgcatt
     3841 cttttgtccc cattgctata tggggtttct cactgactct tccctggaag agcatattag
     3901 acaggttcat tgtgacctca gtggctcccg atttgggtct ccagtgcttg ggactcccaa
     3961 agaaccagta gtagaagtct attcttgttc ctattgtaca aattcgccaa tattcaacag
     4021 cgttcttaaa ctgaacaagc atatcaaaga gaatcataaa aacattccct tggccctgaa
     4081 ttatatccac aatgggaaga aatccagggc cttaagcccc ctatctcctg tggccataga
     4141 gcagacatct cttaagatga tgcaggcagt aggaggtgca cctgcacgtc ccactggaga
     4201 atatatctgt aatcaatgtg gtgctaagta cacatcccta gacagctttc agactcacct
     4261 aaaaactcat ctcgacactg tgcttccaaa attgacctgt cctcagtgca acaaggaatt
     4321 ccccaaccaa gaatccttgc tgaagcatgt taccattcac tttatgatca cttcaacgta
     4381 ttacatctgt gagagttgtg acaagcaatt cacatcagtg gatgaccttc agaaacacct
     4441 gctggacatg cacacctttg tcttctttcg ctgcaccctc tgccaggaag tttttgactc
     4501 aaaagtctcc attcagctcc acttggctgt gaagcacagt aacgaaaaga aagtctatag
     4561 gtgcacatct tgcaactggg acttccgcaa cgaaactgac ttgcagctcc atgtgaaaca
     4621 caaccacctg gaaaaccaag ggaaagtgca taagtgcatt ttctgcggtg agtcctttgg
     4681 caccgaggtg gagctgcaat gccacatcac cactcacagt aagaagtaca actgcaagtt
     4741 ctgtagcaaa gccttccatg cgatcatttt gttagaaaaa cacttgcgag aaaaacactg
     4801 tgtattcgaa accaagacac ccaactgtgg aacaaatgga gcttccgagc aagtgcagaa
     4861 agaggaagtg gagctgcaga ctttgctgac caacagccag gagtcccaca acagtcacga
     4921 tgggagcgaa gaagacgttg acacctctga gcctatgtac ggctgcgaca tttgtggggc
     4981 agcctacact atggaaactt tgctgcagaa tcaccagctc cgagaccaca acatcagacc
     5041 tggagaaagt gccatcgtga aaaagaaagc tgagctcatt aaagggaatt acaagtgcaa
     5101 cgtgtgctct cgaaccttct tctccgaaaa tggcctccgg gaacatatgc agacccacct
     5161 aggccctgtc aaacactaca tgtgccctat ttgcggagag cggtttccct cccttttaac
     5221 tcttactgaa cacaaagtca cgcatagtaa gagtcttgat actggaaact gccggatttg
     5281 caagatgcct ctccagagtg aagaggagtt tttagagcat tgccaaatgc accctgactt
     5341 gaggaattcc ctgacaggct ttcgctgcgt ggtgtgcatg cagacagtga cctccacctt
     5401 ggaactcaaa atccatggga cgttccacat gcaaaagaca gggaatgggt ctgcagttca
     5461 gaccacaggg cggggccagc acgtccaaaa actgtataag tgcgcatctt gcctcaaaga
     5521 attccgttcc aagcaagatc tggtgaaact tgatatcaat ggcctgccat atggtctgtg
     5581 tgccggctgc gtgaatctca gtaagagcgc cagcccaggc attaacgtcc ctcccggcac
     5641 gaatagacca ggcttgggcc agaatgagaa tctgagtgcc attgagggga aaggcaaggt
     5701 ggggggactg aagacacgct gctctagctg caacgttaag tttgagtctg aaagtgaact
     5761 ccagaaccac atccaaacca tccaccgaga gctcgtgcca gacagcaaca gcacacagtt
     5821 gaaaacgccc caagtatcac caatgcccag aatcagtccc tcccagtcgg atgagaagaa
     5881 gacctatcaa tgcatcaaat gtcagatggt tttctacaat gaatgggata ttcaggttca
     5941 tgttgcaaat cacatgattg atgaaggact gaaccatgaa tgcaaactct gcagccagac
     6001 ctttgactct cctgccaaac tccagtgcca cctgatagag cacagcttcg aagggatggg
     6061 aggcaccttc aagtgtccag tctgctttac agtatttgtt caagcaaaca agttgcagca
     6121 gcatattttc tctgcccatg gacaagaaga caagatctat gactgtacac aatgtccaca
     6181 gaagtttttc ttccaaacag agctgcagaa tcatacaatg acccaacaca gcagtggtgg
     6241 aggcggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     6301 ggatgacgat gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg
     6361 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     6421 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     6481 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     6541 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     6601 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     6661 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     6721 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     6781 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     6841 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga
     6901 gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga
     6961 gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat
     7021 gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta
     7081 cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat
     7141 caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca
     7201 cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg
     7261 caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac
     7321 cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt
     7381 cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag
     7441 cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga
     7501 ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc
     7561 catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg
     7621 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
     7681 ggagaatccc ggccctgcca ccatggctga acaagatgga ttgcacgcag gttctccggc
     7741 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     7801 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     7861 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     7921 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     7981 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     8041 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     8101 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     8161 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     8221 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     8281 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8341 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8401 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8461 catcgccttc tatcgccttc ttgacgagtt cttctgagtc gacaatcaac ctctggatta
     8521 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     8581 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     8641 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     8701 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     8761 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     8821 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8881 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     8941 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     9001 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     9061 gagtcggatc tccctttggg ccgcctcccc gcggtacctt taagaccaat gacttacaag
     9121 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     9181 tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     9241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9361 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     9421 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     9481 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9541 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9601 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9661 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9721 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     9781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9841 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9901 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10021 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10081 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10621 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11401 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    11581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    11641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11881 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    11941 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    12001 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    12061 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    12121 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    12181 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    12241 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    12301 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    12361 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
