LOCUS       V031586                10037 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031586
VERSION     V031586
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..3850
                     /label="TRIM6(NM_001003818)"
                     /note="TRIM6(NM_001003818)"
                     /gene="TRIM6"
     CDS             3857..3946
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        3990..4201
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4214..4482
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4511..4520
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4541..5203
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5273..5326
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5333..6127
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6134..6722
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6794..7027
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7099..7220
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7239..7374
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7412..7428)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7436..7452
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7460..7490)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7505..7526
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7814..8402)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8573..9433)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9434..9538)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10012..10028
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtgcgg gtcagagagg attctacagg caggaaacat
     2341 cttagaaatc agggttgggc aggcaggagc caggagagta gctacaatga cttcaccagt
     2401 actggtggac atacgagaag aggtgacctg ccctatctgc ctggagctcc taacagaacc
     2461 cctgagcata gactgtggcc acagcttctg ccaagcctgc atcacaccaa atggcaggga
     2521 atcagtgatt ggtcaagaag gggaaagaag ctgccctgtg tgccagacca gctaccagcc
     2581 agggaacctg cggcctaatc ggcatctggc caacatagtg aggcggctca gagaggtagt
     2641 gttgggccct gggaagcagc tgaaagcagt tctttgtgca gaccatggag aaaaactgca
     2701 gctcttctgt caggaggatg ggaaggtcat ttgctggctt tgtgagcggt ctcaggagca
     2761 ccgtggtcac cacacgttcc tcgtggagga ggttgcccag gagtaccagg agaagtttca
     2821 ggagtctcta aagaagctga agaacgagga gcaggaagct gagaagctaa cagcttttat
     2881 cagagagaag aaaacatcct ggaagaatca gatggagcct gagagatgca ggatccagac
     2941 agagtttaat cagctgcgaa atatcctaga cagagtggag caacgggagc tgaaaaagct
     3001 ggaacaggaa gagaagaagg ggctacgaat tatagaagag gctgagaatg atctggtcca
     3061 ccagacccag tcgctgcgag agctcatctc ggatctggag cgtcgatgtc aggggtcaac
     3121 aatggagctg ctgcaggatg tgagtgatgt cacagaaagg agtgagttct ggaccctgag
     3181 gaagccagaa gctctcccta caaagctgag aagtatgttc cgagccccag atctgaaaag
     3241 gatgctgcga gtgtgtagag agctgacaga tgtccaaagc tactgggttg acgtgaccct
     3301 gaatccacac acagctaatt taaatcttgt cctggctaaa aaccggagac aagtgaggtt
     3361 tgtgggagct aaagtatctg gaccttcctg tctggaaaag cattatgact gtagtgtcct
     3421 gggctcccag cacttctcct ctggtaagca ttactgggag gtagatgtgg ccaagaagac
     3481 tgcctggatc ctgggggtat gcagcaattc actgggacct acattctctt tcaaccattt
     3541 tgctcaaaat cacagtgctt actccaggta tcagcctcag agtggatact gggtgattgg
     3601 gttacagcat aaccatgaat atagggccta tgaggattct tccccttccc tgcttctctc
     3661 catgacagtg ccccctcgcc gtgttggggt tttcttagat tatgaggctg gtactgtctc
     3721 cttttataat gtcacaaacc atggcttccc catctacact ttctctaaat attactttcc
     3781 cactactctt tgtccatatt ttaatccttg caactgtgta attcctatga ccctgcgtcg
     3841 tccaagctct ggatccgaac aaaaactaat atcagaggaa gatttggaac agaaactaat
     3901 cagtgaagaa gatttagagc aaaagttaat ttccgaggag gacttatagc ggccgcgaag
     3961 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     4021 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     4081 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     4141 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     4201 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     4261 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     4321 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     4381 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     4441 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     4501 tacgctagac gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg
     4561 ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac
     4621 ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag
     4681 cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag
     4741 cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat
     4801 cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg
     4861 ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt
     4921 caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa
     4981 cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt
     5041 cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg
     5101 gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat
     5161 cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc
     5221 gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag
     5281 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctgcca ccatggctga
     5341 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5401 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5461 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5521 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5581 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5641 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5701 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5761 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5821 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5881 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5941 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6001 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6061 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6121 cttctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6181 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6241 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6301 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6361 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6421 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6481 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     6541 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6601 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6661 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6721 gcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6781 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct
     6841 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6901 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6961 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7021 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7081 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7201 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7261 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7321 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7381 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7441 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7501 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7561 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8221 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9541 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9601 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     9661 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9781 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9841 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     9901 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     9961 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    10021 cggccagtgc caagctg
//
