LOCUS       V046145                10250 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046145
VERSION     V046145
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4087
                     /label="Ptpn6(NM_013545)"
                     /note="Ptpn6(NM_013545)"
                     /gene="Ptpn6"
     CDS             4094..4159
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4203..4414
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4427..4695
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4724..4733
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4754..5416
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5486..5539
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5546..6340
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6347..6935
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7007..7240
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7312..7433
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7452..7587
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7625..7641)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7649..7665
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7673..7703)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7718..7739
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8027..8615)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8786..9646)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9647..9751)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10225..10241
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggtgag gtggtttcac cgggacctca gcgggcctga
     2341 tgcagagacc ctgctgaagg gccggggagt ccctgggagc ttcctggctc ggcccagccg
     2401 caagaaccag ggtgacttct ccctctcagt cagggtggat gatcaggtga ctcatattcg
     2461 gatccagaac tcaggggact tctatgacct gtacggaggg gagaagtttg cgacgctgac
     2521 agagctggtc gagtattaca cgcagcagca gggcatcctg caggaccgag atggcaccat
     2581 catccacctt aagtacccac tgaactgctc ggaccccacc agtgagaggt ggtaccacgg
     2641 ccacatatct ggagggcagg cggagtcact gctgcaggcc aagggcgagc cctggacatt
     2701 tcttgtgcgt gagagtctca gccaacctgg tgattttgtg ctctctgtgc tcaatgacca
     2761 gcccaaggct ggcccaggtt ccccgctcag ggtcactcat atcaaggtta tgtgtgaggg
     2821 tggacgctat actgtgggtg gctcagagac gtttgacagc ctcacagacc tggtggagca
     2881 cttcaagaag acagggattg aggaggcctc gggtgccttt gtctacctgc ggcagcctta
     2941 ctacgctact cgggtaaacg cagctgacat tgagaatcgg gtcttggaac tgaacaagaa
     3001 gcaggagtcg gaggacacag ccaaggctgg cttctgggag gagtttgaga gtctacaaaa
     3061 gcaggaggta aagaatctac accaacgtct ggaagggcag cggccagaga acaagagcaa
     3121 gaaccgctac aagaacattc ttccctttga ccacagccga gtgatcctgc agggacgtga
     3181 cagtaacatc ccaggctctg actacatcaa tgccaactac gtgaagaacc agctgctagg
     3241 tccagatgag aactctaaga cctacatcgc cagccagggc tgtctggatg ccacagtcaa
     3301 tgacttctgg cagatggctt ggcaggagaa cactcgtgtc atcgtcatga ctaccagaga
     3361 ggtggagaaa ggccggaaca aatgtgtccc atactggccc gaggtgggca ctcagcgtgt
     3421 ctatggtctc tactctgtga ccaacagtag ggagcatgac acagcagaat acaaactgcg
     3481 aacattacag atctccccac tagacaatgg ggacctggtt cgggagatat ggcactacca
     3541 gtacctgagc tggcctgacc atggggttcc cagtgagcct gggggtgtcc tcagctttct
     3601 ggatcagatc aaccagcgac aggaaagttt gcctcatgca gggcccatca ttgtgcattg
     3661 cagcgctggc atcggccgca cgggcaccat catcgtcatt gatatgctta tggaaagcat
     3721 ctccaccaag gggctagact gtgacattga tatccagaag accatccaga tggtacgagc
     3781 acagcgctcc ggcatggtgc agaccgaggc ccagtacaag tttatttacg tggccattgc
     3841 ccagttcatc gaaacgacca agaagaaact ggagatcata caatcccaga agggccagga
     3901 gtcggagtat gggaatatca cgtaccctcc cgctgtgagg agtgcccacg ccaaagcctc
     3961 gcgtacttcc tccaagcaca aggaggaggt gtacgaaaac gtgcatagca agagcaagaa
     4021 ggaagagaaa gtaaagaagc agcggtcggc agacaaggag aagaacaaag gttctctcaa
     4081 gaggaaggga tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     4141 caaggatgac gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     4201 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4261 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4321 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4381 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     4441 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     4501 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     4561 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     4621 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     4681 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga
     4741 cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt
     4801 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa
     4861 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg
     4921 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc
     4981 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct
     5041 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt
     5101 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc
     5161 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac
     5221 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa
     5281 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt
     5341 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac
     5401 ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc
     5461 cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt
     5521 ggaggagaat cccggccctg ccaccatggc tgaacaagat ggattgcacg caggttctcc
     5581 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5641 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5701 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     5761 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5821 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5881 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5941 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6001 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6061 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6121 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6181 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6241 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6301 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gtcgacaatc aacctctgga
     6361 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6421 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6481 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6541 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6601 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6661 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6721 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6781 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6841 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6901 gacgagtcgg atctcccttt gggccgcctc cccgcggtac ctttaagacc aatgacttac
     6961 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7021 cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7081 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7141 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7201 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7261 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7321 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7381 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7441 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7501 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7561 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat
     7621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8461 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9781 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     9841 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     9901 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     9961 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
    10021 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10081 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10141 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10201 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
