LOCUS       V043708                10772 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043708
VERSION     V043708
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4609
                     /label="PGR(NM_001202474)"
                     /note="PGR(NM_001202474)"
                     /gene="PGR"
     CDS             4616..4681
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4725..4936
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4949..5217
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5246..5255
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5276..5938
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6008..6061
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6068..6862
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6869..7457
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7529..7762
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7834..7955
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7974..8109
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8147..8163)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8171..8187
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8195..8225)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8240..8261
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8549..9137)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9308..10168)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10169..10273)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10747..10763
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgagccg gtccgggtgc aaggttggag acagctccgg
     2341 gacggcagct gcccataaag tgctgccccg gggcctgtca ccagcccggc agctgctgct
     2401 cccggcctct gagagccctc actggtccgg ggccccagtg aagccgtctc cgcaggccgc
     2461 tgcggtggag gttgaggagg aggatggctc tgagtccgag gagtctgcgg gtccgcttct
     2521 gaagggcaaa cctcgggctc tgggtggcgc ggcggctgga ggaggagccg cggctgtccc
     2581 gccgggggcg gcagcaggag gcgtcgccct ggtccccaag gaagattccc gcttctcagc
     2641 gcccagggtc gccctggtgg agcaggacgc gccgatggcg cccgggcgct ccccgctggc
     2701 caccacggtg atggatttca tccacgtgcc tatcctgcct ctcaatcacg ccttattggc
     2761 agcccgcact cggcagctgc tggaagacga aagttacgac ggcggggccg gggctgccag
     2821 cgcctttgcc ccgccgcgga gttcaccctg tgcctcgtcc accccggtcg ctgtaggcga
     2881 cttccccgac tgcgcgtacc cgcccgacgc cgagcccaag gacgacgcgt accctctcta
     2941 tagcgacttc cagccgcccg ctctaaagat aaaggaggag gaggaaggcg cggaggcctc
     3001 cgcgcgctcc ccgcgttcct accttgtggc cggtgccaac cccgcagcct tcccggattt
     3061 cccgttgggg ccaccgcccc cgctgccgcc gcgagcgacc ccatccagac ccggggaagc
     3121 ggcggtgacg gccgcacccg ccagtgcctc agtctcgtct gcgtcctcct cggggtcgac
     3181 cctggagtgc atcctgtaca aagcggaggg cgcgccgccc cagcagggcc cgttcgcgcc
     3241 gccgccctgc aaggcgccgg gcgcgagcgg ctgcctgctc ccgcgggacg gcctgccctc
     3301 cacctccgcc tctgccgccg ccgccggggc ggcccccgcg ctctaccctg cactcggcct
     3361 caacgggctc ccgcagctcg gctaccaggc cgccgtgctc aaggagggcc tgccgcaggt
     3421 ctacccgccc tatctcaact acctgaggcc ggattcagaa gccagccaga gcccacaata
     3481 cagcttcgag tcattacctc agaagatttg tttaatctgt ggggatgaag catcaggctg
     3541 tcattatggt gtccttacct gtgggagctg taaggtcttc tttaagaggg caatggaagg
     3601 gcagcacaac tacttatgtg ctggaagaaa tgactgcatc gttgataaaa tccgcagaaa
     3661 aaactgccca gcatgtcgcc ttagaaagtg ctgtcaggct ggcatggtcc ttggaggtcg
     3721 aaaatttaaa aagttcaata aagtcagagt tgtgagagca ctggatgctg ttgctctccc
     3781 acagccagtg ggcgttccaa atgaaagcca agccctaagc cagagattca ctttttcacc
     3841 aggtcaagac atacagttga ttccaccact gatcaacctg ttaatgagca ttgaaccaga
     3901 tgtgatctat gcaggacatg acaacacaaa acctgacacc tccagttctt tgctgacaag
     3961 tcttaatcaa ctaggcgaga ggcaacttct ttcagtagtc aagtggtcta aatcattgcc
     4021 aggttttcga aacttacata ttgatgacca gataactctc attcagtatt cttggatgag
     4081 cttaatggtg tttggtctag gatggagatc ctacaaacac gtcagtgggc agatgctgta
     4141 ttttgcacct gatctaatac taaatgaaca gcggatgaaa gaatcatcat tctattcatt
     4201 atgccttacc atgtggcaga tcccacagga gtttgtcaag cttcaagtta gccaagaaga
     4261 gttcctctgt atgaaagtat tgttacttct taatacaatt cctttggaag ggctacgaag
     4321 tcaaacccag tttgaggaga tgaggtcaag ctacattaga gagctcatca aggcaattgg
     4381 tttgaggcaa aaaggagttg tgtcgagctc acagcgtttc tatcaactta caaaacttct
     4441 tgataacttg catgatcttg tcaaacaact tcatctgtac tgcttgaata catttatcca
     4501 gtcccgggca ctgagtgttg aatttccaga aatgatgtct gaagttattg ctgcacaatt
     4561 acccaagata ttggcaggga tggtgaaacc ccttctcttt cataaaaagg gatccgacta
     4621 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     4681 gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     4741 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     4801 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     4861 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     4921 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     4981 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     5041 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     5101 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     5161 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     5221 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc
     5281 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg
     5341 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg
     5401 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
     5461 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
     5521 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
     5581 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga
     5641 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
     5701 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg
     5761 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
     5821 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa
     5881 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag
     5941 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     6001 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     6061 tgccaccatg gctgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     6121 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     6181 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     6241 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     6301 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     6361 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6421 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6481 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6541 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6601 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6661 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6721 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6781 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6841 ccttcttgac gagttcttct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6901 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6961 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7021 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7081 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7141 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7201 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7261 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7321 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7381 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7441 ttgggccgcc tccccgcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7501 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa
     7561 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7621 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7681 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7741 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7801 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7861 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7921 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7981 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8041 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8101 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     8161 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8221 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8281 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8341 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8401 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8461 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8521 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8581 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8641 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8701 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8761 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8821 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8881 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8941 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9001 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9061 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9121 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9181 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9241 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9301 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9361 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9421 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9481 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9541 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9601 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9661 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9721 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9781 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9841 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9901 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9961 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10021 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10081 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10141 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10201 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10261 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    10321 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    10381 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    10441 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    10501 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    10561 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    10621 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
    10681 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
    10741 gacgttgtaa aacgacggcc agtgccaagc tg
//
