LOCUS       V034292                 9851 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034292
VERSION     V034292
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..3688
                     /label="NFIL3(NM_001289999)"
                     /note="NFIL3(NM_001289999)"
                     /gene="NFIL3"
     CDS             3695..3760
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        3804..4015
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4028..4296
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4325..4334
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4355..5017
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5087..5140
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5147..5941
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    5948..6536
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6608..6841
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6913..7034
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7053..7188
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7226..7242)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7250..7266
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7274..7304)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7319..7340
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7628..8216)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8387..9247)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9248..9352)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9826..9842
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgcagct gagaaaaatg cagaccgtca aaaaggagca
     2341 ggcgtctctt gatgccagta gcaatgtgga caagatgatg gtccttaatt ctgctttaac
     2401 ggaagtgtca gaagactcca caacaggtga ggagctgctt ctcagtgaag gaagtgtggg
     2461 gaagaacaaa tcttctgcat gtcggaggaa acgggaattc attcctgatg aaaagaaaga
     2521 tgctatgtat tgggaaaaaa ggcggaaaaa taatgaagct gccaaaagat ctcgtgagaa
     2581 gcgtcgactg aatgacctgg ttttagagaa caaactaatt gcactgggag aagaaaacgc
     2641 cactttaaaa gctgagctgc tttcactaaa attaaagttt ggtttaatta gctccacagc
     2701 atatgctcaa gagattcaga aactcagtaa ttctacagct gtgtactttc aagattacca
     2761 gacttccaaa tccaatgtga gttcatttgt ggacgagcac gaaccctcga tggtgtcaag
     2821 tagttgtatt tctgtcatta aacactctcc acaaagctcg ctgtccgatg tttcagaagt
     2881 gtcctcagta gaacacacgc aggagagctc tgtgcaggga agctgcagaa gtcctgaaaa
     2941 caagttccag attatcaagc aagagccgat ggaattagag agctacacaa gggagccaag
     3001 agatgaccga ggctcttaca cagcgtccat ctatcaaaac tatatgggga attctttctc
     3061 tgggtactca cactctcccc cactactgca agtcaaccga tcctccagca actccccgag
     3121 aacgtcggaa actgatgatg gtgtggtagg aaagtcatct gatggagaag acgagcaaca
     3181 ggtccccaag ggccccatcc attctccagt tgaactcaag catgtgcatg caactgtggt
     3241 taaagttcca gaagtgaatt cctctgcctt gccacacaag ctccggatca aagccaaagc
     3301 catgcagatc aaagtagaag cctttgataa tgaatttgag gccacgcaaa aactttcctc
     3361 acctattgac atgacatcta aaagacattt cgaactcgaa aagcatagtg ccccaagtat
     3421 ggtacattct tctcttactc ctttctcagt gcaagtgact aacattcaag attggtctct
     3481 caaatcggag cactggcatc aaaaagaact gagtggcaaa actcagaata gtttcaaaac
     3541 tggagttgtt gaaatgaaag acagtggcta caaagtttct gacccagaga acttgtattt
     3601 gaagcagggg atagcaaact tatctgcaga ggttgtctca ctcaagagac ttatagccac
     3661 acaaccaatc tctgcttcag actctggggg atccgactac aaagaccatg acggtgatta
     3721 taaagatcat gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg
     3781 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     3841 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     3901 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     3961 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     4021 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     4081 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     4141 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     4201 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     4261 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     4321 agacgccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat
     4381 caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa
     4441 gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta
     4501 cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta
     4561 cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa
     4621 gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt
     4681 gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga
     4741 caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct
     4801 ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt
     4861 ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc
     4921 catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga
     4981 gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa
     5041 ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag
     5101 tcttctaaca tgcggtgacg tggaggagaa tcccggccct gccaccatgg ctgaacaaga
     5161 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5221 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5281 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5341 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5401 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5461 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5521 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5581 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5641 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     5701 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5761 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5821 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5881 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5941 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6001 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6061 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6121 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6181 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6241 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6301 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6361 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6421 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6481 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcggta
     6541 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6601 gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt tgcttgtact
     6661 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6721 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6781 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6841 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6901 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6961 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7021 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     7081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     7201 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7261 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7321 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7381 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7441 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7501 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7561 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7801 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8041 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8221 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8281 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8341 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8401 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8461 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8521 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8581 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8641 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8701 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8761 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8821 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8881 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8941 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9001 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9061 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9121 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9181 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9241 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9301 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9361 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9421 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9481 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9541 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9601 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9661 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     9721 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     9781 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     9841 gtgccaagct g
//
