LOCUS       V029888                 9827 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029888
VERSION     V029888
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..3664
                     /label="MYC(NM_002467)"
                     /note="MYC(NM_002467)"
                     /gene="MYC"
     CDS             3671..3736
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        3780..3991
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4004..4272
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4301..4310
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4331..4993
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5063..5116
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5123..5917
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    5924..6512
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6584..6817
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6889..7010
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7029..7164
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7202..7218)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7226..7242
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7250..7280)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7295..7316
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7604..8192)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8363..9223)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9224..9328)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9802..9818
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggattt ttttcgggta gtggaaaacc agcagcctcc
     2341 cgcgacgatg cccctcaacg ttagcttcac caacaggaac tatgacctcg actacgactc
     2401 ggtgcagccg tatttctact gcgacgagga ggagaacttc taccagcagc agcagcagag
     2461 cgagctgcag cccccggcgc ccagcgagga tatctggaag aaattcgagc tgctgcccac
     2521 cccgcccctg tcccctagcc gccgctccgg gctctgctcg ccctcctacg ttgcggtcac
     2581 acccttctcc cttcggggag acaacgacgg cggtggcggg agcttctcca cggccgacca
     2641 gctggagatg gtgaccgagc tgctgggagg agacatggtg aaccagagtt tcatctgcga
     2701 cccggacgac gagaccttca tcaaaaacat catcatccag gactgtatgt ggagcggctt
     2761 ctcggccgcc gccaagctcg tctcagagaa gctggcctcc taccaggctg cgcgcaaaga
     2821 cagcggcagc ccgaaccccg cccgcggcca cagcgtctgc tccacctcca gcttgtacct
     2881 gcaggatctg agcgccgccg cctcagagtg catcgacccc tcggtggtct tcccctaccc
     2941 tctcaacgac agcagctcgc ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc
     3001 gtcctcggat tctctgctct cctcgacgga gtcctccccg cagggcagcc ccgagcccct
     3061 ggtgctccat gaggagacac cgcccaccac cagcagcgac tctgaggagg aacaagaaga
     3121 tgaggaagaa atcgatgttg tttctgtgga aaagaggcag gctcctggca aaaggtcaga
     3181 gtctggatca ccttctgctg gaggccacag caaacctcct cacagcccac tggtcctcaa
     3241 gaggtgccac gtctccacac atcagcacaa ctacgcagcg cctccctcca ctcggaagga
     3301 ctatcctgct gccaagaggg tcaagttgga cagtgtcaga gtcctgagac agatcagcaa
     3361 caaccgaaaa tgcaccagcc ccaggtcctc ggacaccgag gagaatgtca agaggcgaac
     3421 acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg
     3481 tgaccagatc ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa
     3541 agccacagca tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga
     3601 cttgttgcgg aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg
     3661 tgcgggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     3721 ggatgacgat gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg
     3781 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     3841 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     3901 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     3961 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     4021 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     4081 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     4141 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     4201 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     4261 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga
     4321 gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga
     4381 gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat
     4441 gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta
     4501 cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat
     4561 caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca
     4621 cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg
     4681 caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac
     4741 cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt
     4801 cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag
     4861 cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga
     4921 ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc
     4981 catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg
     5041 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
     5101 ggagaatccc ggccctgcca ccatggctga acaagatgga ttgcacgcag gttctccggc
     5161 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5221 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5281 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5341 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5401 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5461 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5521 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5581 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5641 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5701 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5761 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5821 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5881 catcgccttc tatcgccttc ttgacgagtt cttctgagtc gacaatcaac ctctggatta
     5941 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6001 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6061 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6121 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6181 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6241 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6301 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6361 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6421 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6481 gagtcggatc tccctttggg ccgcctcccc gcggtacctt taagaccaat gacttacaag
     6541 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6601 tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6781 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6841 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7021 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7081 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7141 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     7201 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7261 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7321 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8041 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9361 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9421 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9541 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9601 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9661 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9721 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     9781 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
