LOCUS       V023205                10802 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023205
VERSION     V023205
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2369..4639
                     /label="MFN2(NM_014874)"
                     /note="MFN2(NM_014874)"
                     /gene="MFN2"
     CDS             4646..4711
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4755..4966
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4979..5247
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5276..5285
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5306..5968
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6038..6091
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6098..6892
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6899..7487
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7559..7792
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7864..7985
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8004..8139
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8177..8193)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8201..8217
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8225..8255)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8270..8291
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8579..9167)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9338..10198)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10199..10303)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10777..10793
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgctgct atccgtgccg ttgctgctcg gcctcctcgg
     2341 cctggccgtc gccgaccggt cgcacaccat gtccctgctc ttctctcgat gcaactctat
     2401 cgtcacagtc aagaaaaata agagacacat ggctgaggtg aatgcatccc cacttaagca
     2461 ctttgtcact gccaagaaga agatcaatgg catttttgag cagctggggg cctacatcca
     2521 ggagagcgcc accttccttg aagacacgta caggaatgca gaactggacc ccgttaccac
     2581 agaagaacag gttctggacg tcaaaggtta cctatccaaa gtgagaggca tcagtgaggt
     2641 gctggctcgg aggcacatga aagtggcttt ttttggccgg acgagcaatg ggaagagcac
     2701 cgtgatcaat gccatgctct gggacaaagt tctgccctct gggattggcc acaccaccaa
     2761 ttgcttcctg cgggtagagg gcacagatgg ccatgaggcc tttctcctta ccgagggctc
     2821 agaggaaaag aggagtgcca agactgtgaa ccagctggcc catgccctcc accaggacaa
     2881 gcagctccat gccggcagcc tagtgagtgt gatgtggccc aactctaagt gcccacttct
     2941 gaaggatgac ctcgttttga tggacagccc tggtattgat gtcaccacag agctggacag
     3001 ctggattgac aagttttgtc tggatgctga tgtgtttgtg ctggtggcca actcagagtc
     3061 caccctgatg cagacggaaa agcacttctt ccacaaggtg agtgagcgtc tctcccggcc
     3121 aaacatcttc atcctgaaca accgctggga tgcatctgcc tcagagcccg agtacatgga
     3181 ggaggtgcgg cggcagcaca tggagcgttg taccagcttc ctggtggatg agctgggcgt
     3241 ggtggatcga tcccaggccg gggaccgcat cttctttgtg tctgctaagg aggtgctcaa
     3301 cgccaggatt cagaaagccc agggcatgcc tgaaggaggg ggcgctctcg cagaaggctt
     3361 tcaagtgagg atgtttgagt ttcagaattt tgagaggaga tttgaggagt gcatctccca
     3421 gtctgcagtg aagaccaagt ttgagcagca cacggtccgg gccaagcaga ttgcagaggc
     3481 ggttcgactc atcatggact ccctgcacat ggcggctcgg gagcagcagg tttactgcga
     3541 ggaaatgcgt gaagagcggc aagaccgact gaaatttatt gacaaacagc tggagctctt
     3601 ggctcaagac tataagctgc gaattaagca gattacggag gaagtggaga ggcaggtgtc
     3661 gactgcaatg gccgaggaga tcaggcgcct ctctgtactg gtggacgatt accagatgga
     3721 cttccaccct tctccagtag tcctcaaggt ttataagaat gagctgcacc gccacataga
     3781 ggaaggactg ggtcgaaaca tgtctgaccg ctgctccacg gccatcacca actccctgca
     3841 gaccatgcag caggacatga tagatggctt gaaacccctc cttcctgtgt ctgtgcggag
     3901 tcagatagac atgctggtcc cacgccagtg cttctccctc aactatgacc taaactgtga
     3961 caagctgtgt gctgacttcc aggaagacat tgagttccat ttctctctcg gatggaccat
     4021 gctggtgaat aggttcctgg gccccaagaa cagccgtcgg gccttgatgg gctacaatga
     4081 ccaggtccag cgtcccatcc ctctgacgcc agccaacccc agcatgcccc cactgccaca
     4141 gggctcgctc acccaggagg agttcatggt ttccatggtt accggcctgg cctccttgac
     4201 atccaggacc tccatgggca ttcttgttgt tggaggagtg gtgtggaagg cagtgggctg
     4261 gcggctcatt gccctctcct ttgggctcta tggcctcctc tacgtctatg agcgtctgac
     4321 ctggaccacc aaggccaagg agagggcctt caagcgccag tttgtggagc atgccagcga
     4381 gaagctgcag cttgtcatca gctacactgg ctccaactgc agccaccaag tccagcagga
     4441 actgtctggg acctttgctc atctgtgtca gcaagttgac gtcacccggg agaacctgga
     4501 gcaggaaatt gccgccatga acaagaaaat tgaggttctt gactcacttc agagcaaagc
     4561 aaagctgctc aggaataaag ccggttggtt ggacagtgag ctcaacatgt tcacacacca
     4621 gtacctgcag cccagcagag gatccgacta caaagaccat gacggtgatt ataaagatca
     4681 tgacatcgac tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc
     4741 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4801 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4861 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     4921 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     4981 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     5041 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     5101 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     5161 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     5221 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac
     5281 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac
     5341 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg
     5401 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag
     5461 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc
     5521 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga
     5581 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga
     5641 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat
     5701 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag
     5761 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca
     5821 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc
     5881 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca
     5941 cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt
     6001 ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac
     6061 atgcggtgac gtggaggaga atcccggccc tgccaccatg gctgaacaag atggattgca
     6121 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     6181 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     6241 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     6301 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     6361 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6421 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6481 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6541 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6601 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     6661 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6721 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6781 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6841 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagtcgacaa
     6901 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6961 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7021 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7081 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7141 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7201 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7261 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7321 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7381 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7441 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcggt acctttaaga
     7501 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7561 gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac tgggtctctc
     7621 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7681 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7741 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7801 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7861 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7981 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     8041 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8101 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
     8161 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8221 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8281 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8341 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8401 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8461 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8521 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8581 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8641 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8701 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8761 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8821 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8881 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8941 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9001 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9061 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9121 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9181 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9241 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9301 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9361 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9421 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9481 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9541 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9601 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9661 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9721 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9781 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9841 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9901 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9961 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10021 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10081 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10141 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10201 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10261 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10321 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10381 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10441 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10501 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    10561 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10621 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10681 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10741 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    10801 tg
//
