LOCUS       V038253                11364 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038253
VERSION     V038253
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2285..3586
                     /label="FAM83A(NM_032899)"
                     /note="FAM83A(NM_032899)"
                     /gene="FAM83A"
     CDS             3629..5281
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL*"
                     /label="luciferase"
     promoter        5328..5539
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5552..5820
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5849..5858
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5879..6541
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6611..6664
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6659..7459
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    7460..8047
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8121..8354
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8426..8547
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8566..8701
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8739..8755)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8763..8779
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8787..8817)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8832..8853
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9141..9729)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9900..10760)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10761..10865)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11339..11355
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatgagc cggtcaaggc acctgggcaa aatccggaag cgtctggaag atgtcaagag
     2341 ccagtgggtc cggccagcca gggctgactt tagtgacaac gagagtgccc ggctggccac
     2401 ggacgccctc ttggatgggg gttctgaagc ctactggcgg gtgctcagcc aggaaggcga
     2461 ggtggacttc ttgtcctcgg tggaggccca gtacatccag gcccaggcca gggagccccc
     2521 gtgtccccca gacaccctgg gaggggcgga agcaggccct aagggactgg actccagctc
     2581 cctacagtcc ggcacctact tccctgtggc ctcagagggc agcgagccgg ccctactgca
     2641 cagctgggcc tcagctgaga agccctacct gaaggaaaaa tccagcgcca ctgtgtactt
     2701 ccagaccgtc aagcacaaca acatcagaga cctcgtccgc cgctgcatca cccggactag
     2761 ccaggtcctg gtcatcctga tggatgtgtt cacggatgtg gagatcttct gtgacattct
     2821 agaggcagcc aacaagcgtg gggtgttcgt ttgtgtgctc ctggaccagg gaggtgtgaa
     2881 gctcttccag gagatgtgtg acaaagtcca gatctctgac agtcacctca agaacatttc
     2941 catccggagt gtggaaggag agatatactg tgccaagtca ggcaggaaat tcgctggcca
     3001 aatccgggag aagttcatca tctcggactg gagatttgtc ctgtctggat cttacagctt
     3061 cacctggctc tgcggacacg tgcaccggaa catcctctcc aagttcacag gccaggcggt
     3121 ggagctgttt gacgaggagt tccgccacct ctacgcctcc tccaagcctg tgatgggcct
     3181 gaagtccccg cggctggtcg cccccgtccc gcccggagca gccccggcca atggccgcct
     3241 tagcagcagc agtggctccg ccagtgaccg cacgtcctcc aaccccttca gcggccgctc
     3301 ggcaggcagc caccccggta cccgaagtgt gtccgcgtct tcagggccct gtagccccgc
     3361 ggccccacac ccgcctccac cgccccggtt ccagccccac caaggccctt ggggagcccc
     3421 gagtccccag gcccacctct ccccgcggcc ccacgacggc ccgcccgccg ctgtctacag
     3481 caacctgggg gcctacaggc ccacgcggct gcagctggag cagctgggcc tggtgccgag
     3541 gctgactcca acctggaggc ccttcctgca ggcctcccct cacttcggtg gaggcggatc
     3601 aggaggcggt ggatctgaat tcgccaccat ggaagacgcc aaaaacataa agaaaggccc
     3661 ggcgccattc tatcctcttg aggatggaac cgctggagag caactgcata aggctatgaa
     3721 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtgaacat
     3781 cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
     3841 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
     3901 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
     3961 acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa
     4021 ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga aaattattat
     4081 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
     4141 tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc gtgacaaaac
     4201 aattgcactg ataatgaact cctctggatc tactgggtta cctaagggtg tggcccttcc
     4261 gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg gcaatcaaat
     4321 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     4381 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     4441 gctgttttta cgatcccttc aggattacaa aattcaaagt gcgttgctag taccaaccct
     4501 attttcattc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     4561 aattgcttct gggggcgcac ctctttcgaa agaagtcggg gaagcggttg caaaacgctt
     4621 ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag ctattctgat
     4681 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     4741 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag gcgaattatg
     4801 tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     4861 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     4921 cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatacc aggtggcccc
     4981 cgctgaattg gagtcgatat tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg
     5041 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     5101 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     5161 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     5221 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt ccaaattgtg
     5281 aggatccgcg gccgcaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     5341 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     5401 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     5461 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     5521 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     5581 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     5641 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     5701 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     5761 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     5821 caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc
     5881 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     5941 gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa
     6001 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     6061 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     6121 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     6181 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc
     6241 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     6301 gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga
     6361 cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     6421 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag
     6481 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc
     6541 cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac
     6601 cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     6661 ccctatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     6721 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6781 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6841 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     6901 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     6961 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7021 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7081 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7141 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     7201 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     7261 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     7321 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     7381 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     7441 tcttgacgag ttcttctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7501 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7561 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7621 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7681 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7741 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7801 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7861 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7921 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7981 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc
     8041 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8101 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     8161 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8221 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8281 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8341 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8401 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8521 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8581 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8641 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8701 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     8761 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8821 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8881 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8941 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9001 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9061 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9541 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9661 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9721 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9781 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9841 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10201 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10501 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10861 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    10921 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    10981 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    11041 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    11101 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    11161 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
    11221 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    11281 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    11341 aaaacgacgg ccagtgccaa gctg
//
