LOCUS       V041296                 9872 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041296
VERSION     V041296
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..3709
                     /label="ETS2(NM_005239)"
                     /note="ETS2(NM_005239)"
                     /gene="ETS2"
     CDS             3716..3781
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        3825..4036
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4049..4317
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4346..4355
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4376..5038
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5108..5161
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5168..5962
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    5969..6557
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6629..6862
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6934..7055
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7074..7209
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7247..7263)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7271..7287
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7295..7325)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7340..7361
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7649..8237)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8408..9268)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9269..9373)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9847..9863
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgaatga tttcggaatc aagaatatgg accaggtagc
     2341 ccctgtggct aacagttaca gagggacact caagcgccag ccagcctttg acacctttga
     2401 tgggtccctg tttgctgttt ttccttctct aaatgaagag caaacactgc aagaagtgcc
     2461 aacaggcttg gattccattt ctcatgactc cgccaactgt gaattgcctt tgttaacccc
     2521 gtgcagcaag gctgtgatga gtcaagcctt aaaagctacc ttcagtggct tcaaaaagga
     2581 acagcggcgc ctgggcattc caaagaaccc ctggctgtgg agtgagcaac aggtatgcca
     2641 gtggcttctc tgggccacca atgagttcag tctggtgaac gtgaatctgc agaggttcgg
     2701 catgaatggc cagatgctgt gtaaccttgg caaggaacgc tttctggagc tggcacctga
     2761 ctttgtgggt gacattctct gggaacatct ggagcaaatg atcaaagaaa accaagaaaa
     2821 gacagaagat caatatgaag aaaattcaca cctcacctcc gttcctcatt ggattaacag
     2881 caatacatta ggttttggca cagagcaggc gccctatgga atgcagacac agaattaccc
     2941 caaaggcggc ctcctggaca gcatgtgtcc ggcctccaca cccagcgtac tcagctctga
     3001 gcaggagttt cagatgttcc ccaagtctcg gctcagctcc gtcagcgtca cctactgctc
     3061 tgtcagtcag gacttcccag gcagcaactt gaatttgctc accaacaatt ctgggactcc
     3121 caaagaccac gactcccctg agaacggtgc ggacagcttc gagagctcag actccctcct
     3181 ccagtcctgg aacagccagt cgtccttgct ggatgtgcaa cgggttcctt ccttcgagag
     3241 cttcgaagat gactgcagcc agtctctctg cctcaataag ccaaccatgt ctttcaagga
     3301 ttacatccaa gagaggagtg acccagtgga gcaaggcaaa ccagttatac ctgcagctgt
     3361 gctggccggc ttcacaggaa gtggacctat tcagctgtgg cagtttctcc tggagctgct
     3421 atcagacaaa tcctgccagt cattcatcag ctggactgga gacggatggg agtttaagct
     3481 cgccgacccc gatgaggtgg cccgccggtg gggaaagagg aaaaataagc ccaagatgaa
     3541 ctacgagaag ctgagccggg gcttacgcta ctattacgac aagaacatca tccacaagac
     3601 gtcggggaag cgctacgtgt accgcttcgt gtgcgacctc cagaacttgc tggggttcac
     3661 gcccgaggaa ctgcacgcca tcctgggcgt ccagcccgac acggaggacg gatccgacta
     3721 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     3781 gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     3841 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     3901 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     3961 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     4021 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     4081 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     4141 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     4201 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     4261 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     4321 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc
     4381 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg
     4441 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg
     4501 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
     4561 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
     4621 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
     4681 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga
     4741 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
     4801 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg
     4861 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
     4921 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa
     4981 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag
     5041 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     5101 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     5161 tgccaccatg gctgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5221 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5281 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5341 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5401 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5461 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5521 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5581 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5641 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5701 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5761 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5821 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5881 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5941 ccttcttgac gagttcttct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6001 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6061 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6121 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6181 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6241 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6301 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6361 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6421 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6481 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6541 ttgggccgcc tccccgcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6601 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa
     6661 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6721 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6781 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6841 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6901 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7201 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     7261 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7321 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7381 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7441 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7561 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8161 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8221 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8281 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8341 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8401 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8461 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8521 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8581 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8641 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8701 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8761 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8821 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8881 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8941 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9001 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9061 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9121 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9181 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9241 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9301 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9361 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     9421 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     9481 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9541 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9601 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     9721 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9781 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9841 gacgttgtaa aacgacggcc agtgccaagc tg
//
