LOCUS       V029263                11365 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029263
VERSION     V029263
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5391
                     /label="Dpyd(NM_170778)"
                     /note="Dpyd(NM_170778)"
                     /gene="Dpyd"
     CDS             5398..5463
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5507..5718
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5731..5999
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6028..6037
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6058..6720
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6790..6843
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6844..7443
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7444..8032
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8106..8339
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8411..8532
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8572..8707
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8740..8756)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8764..8780
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8788..8818)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8833..8854
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9142..9730)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9901..10761)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10762..10866)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11340..11356
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgggtgtgct gagcagggac
     2341 gcgccggaca tcgagagtat cctggcttta aaccctcgag tacaagctca tgcaactctg
     2401 cgttccaccg cagccaagaa actagacaag aaacactgga aaaggaacac ggacaagaac
     2461 tgctttacct gtgagaagct ggagagtaat tttgatgaca tcaagcacac gactcttggt
     2521 gagcgaggag ctctccgaga agcagtgaga tgcctgaagt gcgcagatgc tccctgccag
     2581 aagagctgtc ccacatctct tgacattaaa tcatttatca caagtatcgc caacaagaac
     2641 tactatggtg cagctaagct gattttttct gataaccctc ttggtcttac ttgtggaatg
     2701 gtttgtccaa catctgacct ctgtgttgga ggatgcaact tacatgctgc tgaagaggga
     2761 ccaattaata ttggtgggct gcagcagttt gctactgagg tgttcaaagc aatgaacatc
     2821 ccacagatca gaaacccatc gctgcctcct ccggaacata tgccggaagc ctactcagca
     2881 aaaattgcac tttttggtgc agggcctgcg agtataagct gtgcctcctt tttggctcgg
     2941 ttgggctatt ccaacattac tatattcgaa aaacaagaat atgttggtgg tttaagcact
     3001 tctgaaatcc ctcagtttcg actcccatat gacgtcgtga attttgagat tgagctcatg
     3061 aaggaccttg gtgtaaagat aatttgtggt aaaagccttt ccaccgatga aatgactctt
     3121 agctctttga aagaaaatgg ctacagagct gcatttattg gaataggctt gccagaaccc
     3181 aaaaaggacc atattttcca aggcttgaca caagtccagg gattttacac atctaaagac
     3241 tttctgccac ttgtcgccaa aagcagtaaa acaggaatgt gcgcctgtca ctctccattg
     3301 ccatccataa ggggagccgt gattgtactc ggagctggag atactgcgtt tgactgtgca
     3361 acatccgctc tgcgctgtgg agcccttcgc gtgttcatcg tcttcagaaa gggctttgtt
     3421 aatattcgag ctgttccaga ggagatggag ctcgctaagg aagagaaatg tgaatttttg
     3481 cctttcctct cgccacggaa ggttatagtc aaagatggaa agattgtggc aatgcaattt
     3541 gttcgaactg agcaagatga aaccggaaac tgggtggaag atgaagagca gacggtgcgg
     3601 ctaaaggctg atgtggttat tagtgccttt ggatctgtcc tggaggatcc caaagtgaaa
     3661 gaagcattga gtcccatcaa gtttaacaga tggggtctcc cagaagtaaa tccagaaacc
     3721 atgcaaacca gtgaaccatg ggtgtttgca ggtggtgatg ttgtgggtat ggctaacacc
     3781 acagtggaat ctgtcaacga tggaaagcaa gcttcatggt acattcacaa gcacatacag
     3841 gcacaatatg gaacctcagt gccttctcag cctacaatgc ccctatttta cactccggtt
     3901 gacttagtgg acatcagcgt ggaaatggca gggttgaggt ttcccaatcc ctttggcctt
     3961 gccagtgcga caccagccac tagcacacca atgattcgaa gggcctttga agctggctgg
     4021 ggttttgctc tgaccaaaac tttctctctt gataaggaca tcgtgacaaa cgtctcacca
     4081 agaatcatcc gggggacaac ttctggcccc ttgtatggcc ctggacaaag ctcctttctt
     4141 aacattgagc tcatcagtga gaaaacagct gcatattggt gtcacagtgt caccgaacta
     4201 aaggctgact tcccggacaa tatcctgatc gccagcatca tgtgcagtta caacaagagt
     4261 gactggatgg aactctccaa aatggctgag gcttctggag cagatgccct ggagttaaat
     4321 ttatcgtgtc cacatggcat gggggagaga ggaatgggcc tggcttgtgg gcaggatcca
     4381 gagctggtga ggaacatctg tcgctgggtt aggcaagctg ttcgggttcc attttttgcc
     4441 aagttgaccc caaatgtcac tgatattgta agcatcgcaa gagcagcaaa ggaaggtgga
     4501 gcagatggtg taacagccac caacaccgtc tcgggtctga tgggactgaa agctgatggc
     4561 acaccctggc ctgctgtggg cattggaagg aggactacat atggaggagt gtcaggaact
     4621 gctatcaggc ccattgcttt gagagctgtg accgccattg cccgtgcttt gcctgggttt
     4681 cctattttgg ccacaggtgg aattgactca gctgaaagtg gacttcagtt tcttcatagt
     4741 ggtgcttcag ttcttcaggt atgcagcgct attcagaatc aggacttcac tgtgattgaa
     4801 gattactgca ctggactcaa agccctgctt tatctgaaga gcattgaaga gttagcagac
     4861 tgggatggtc agagtccacc cattataagt catcagaaag ggaaaccagt tccacgcgtt
     4921 gctgagctca tgggacagaa actgcccagc tttggacctt accttgaaca gcgcaagaaa
     4981 atcatagcag caagcaaaat cagacagaaa gatcaaaaca cagcttgctc acctctccag
     5041 agaaagcact ttaactcgca aaagcctatt cctgccatca aggatgtaat tggaaaatca
     5101 ctgcaatacc tgggaacatt tggtgagatg agcatcatgg aacaagttgt ggccctgatc
     5161 gatgaggaaa tgtgcatcaa ttgcggcaaa tgttacatga cctgtaatga ctctggctac
     5221 caggctatcc agttcgatcc agaaactcac ctgcctaccg ttagcgacac atgtacaggc
     5281 tgtactctct gcctcagcgt ctgccctatt atggactgta tcaggatggt ttccagggca
     5341 acaccttatc aaccaaagag aggcctacca ttagccgtga agccggtgtg tggatccgac
     5401 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     5461 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     5521 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     5581 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5641 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5701 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     5761 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     5821 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     5881 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     5941 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     6001 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     6061 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     6121 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     6181 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     6241 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     6301 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     6361 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     6421 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     6481 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     6541 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     6601 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     6661 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     6721 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     6781 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     6841 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6901 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6961 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     7021 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     7081 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     7141 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     7201 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     7261 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7321 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7381 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7441 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7501 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7561 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7621 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7681 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7741 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7801 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7861 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7921 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7981 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     8041 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8101 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     8161 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8221 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8281 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8341 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8401 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8521 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     8581 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8641 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8701 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     8761 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8821 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8881 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8941 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9001 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9061 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9121 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9181 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9241 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9301 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9361 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9421 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9481 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9541 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     9601 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9661 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9721 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9781 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9841 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9901 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9961 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10021 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10081 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10141 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10201 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10261 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10321 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10381 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10441 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10501 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10561 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10621 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10681 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10741 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10801 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10861 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10921 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    10981 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    11041 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    11101 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    11161 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    11221 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    11281 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    11341 taaaacgacg gccagtgcca agctg
//
