LOCUS       V026153                10588 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026153
VERSION     V026153
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4614
                     /label="Dennd1b(NM_001166501)"
                     /note="Dennd1b(NM_001166501)"
                     /gene="Dennd1b"
     CDS             4621..4686
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4730..4941
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4954..5222
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5251..5260
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5281..5943
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6013..6066
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6067..6666
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6667..7255
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7329..7562
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7634..7755
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7795..7930
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7963..7979)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7987..8003
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8011..8041)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8056..8077
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8365..8953)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9124..9984)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9985..10089)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10563..10579
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg accgcaggac cagggaaaat
     2341 ccagaaagaa cttttgactt ggtattgaaa gtgaagtgtc atgcctctga aaatgaagat
     2401 cctgaggtat tgtggaaatt cccagaggac tttggagacc aggaagttct gcagagcgtg
     2461 cccaagttct gttttccctt cgatgtggaa agggtgtctc agaatcaagt tggacagcac
     2521 tttaccttcg tcctgacaga catggaaagt aagcagcgat ttgggttctg caggctcaca
     2581 tctggaggca gaatttgttt atgtattctt agttacttgc catggtttga agtatattat
     2641 aaacttctaa atactctggc agattacttg gccaaggaac tggaagagga tttgaatgaa
     2701 actcttaaat cactctataa ccacccagta ccaaaggcaa acactcctgt caacttgagt
     2761 gtgcactcct gtttcatcac acctgacata actggactcc caacgatacc ggagagtaga
     2821 aacctgaccg agtactttgt tgccgtggat gtcaacaaca tgctgcgact gtacgccagc
     2881 atgctgcatg agaggcgcat cattattacc tcgagcaaat taagcacttt aactgcctgt
     2941 ctccatggat cggctgcgct actctacccg atgtactggc aacacatata tatccccgtg
     3001 cttcctccac acctgctgga ctactgctgt gccccaatgc catacctgat tggaatacac
     3061 tccagtctca tagagagagt gaaaaataaa tcattggaag atgtggttgt gttaaatgtt
     3121 gacacaaaca cgttagaatc accatttaat gacttgagca gcctgcccag tgatgtggtc
     3181 tcggccttga aaaataagct gaagaagcag tccacagcta cgggagatgg agtggctcgg
     3241 gcctttctga gggctcaggc ggctttgttt ggctcctacc gagatgctct gagatacaag
     3301 cctggcgagc ccatcacctt ctgtgaggag agctttgtaa agcaccgctc aagcgtgatg
     3361 aaacagttcc tggagactgc agttaacctt cagctcttta agcagtttat tgatggtcga
     3421 ctggcaaagc ttaacgcagg aagaggtttc tctgatatct tcgaagaaga gatcacttcg
     3481 ggtggcttct gtggagggag cccgaggtca tatcagcagt gggtgtatac agtcaagaaa
     3541 ggaggtgcat tgttcaacac agcagtgacc aaagcaactc ccgctgtgcg gactgcatac
     3601 aaatttgcaa agagtcacgc cagactggga ctaaaggagg tgaagagcag gctgaaacac
     3661 aaggacaatg aggaagagta tgggacctgt tctggtttgg tgcagtatac accagtgtac
     3721 actttacaca atgaaaaggg agagaacaga gagaagcaca agctttccca gacacacttg
     3781 aaaaggcctc ataagagcct agatggtacc ctgtatgatg atgatgatga tgacgatgac
     3841 attgaacggg caagcaagat atcttctgaa gacggggaag aaacccgtgc ttatttctac
     3901 gaaagcgatg actccgttga agcccaggtg aaggctcctt actcagggga gatggactta
     3961 ctgggtgaga tcctggacac gctgagcacg cacagctctg atcaaggaaa gctggcgcct
     4021 gccaagagcc tggatttctt cagatccatg gatgacattg attacaagcc tacgaacaag
     4081 tccaacgctc ccagtgagaa taacctggcc ctgctgtgtg cttctggaga ccagggagag
     4141 tggaatctcg ggcaggacga cagcgccctc catggcaggc aactccctcc atccccaagg
     4201 aaacgcgttt cctctggtgg gctgacagag tctctgttta tcctgaaaga ggagagcagg
     4261 gagaagcccc tctgtgctga cagcgtaagt ggccctactg tggtgggaaa gccagctccg
     4321 acttcaggac tgcgttccca gcctgctgct cctgaagctt cccaaacaga gagaggaaga
     4381 gcagaagtga agcaaacacc aggtcaggct ccacttcagt cagaggatct gtcggtgccc
     4441 ggccccggga gccgccagtc gacttttgtt ccctgggaga aggctggcaa agaggacacg
     4501 aagccttcaa aggatgtcgg acttctccaa gaagtagtgt cattgtgtca catgtcatgt
     4561 gacttccaac aagatttaaa catttcagaa gaaagcagaa gtggaaacca aactggatcc
     4621 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     4681 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4741 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4801 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4861 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4921 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4981 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5041 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5101 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5161 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5221 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     5281 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     5341 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5401 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5461 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5521 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5581 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5641 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5701 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5761 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5821 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5881 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5941 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     6001 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     6061 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6121 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6181 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6241 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6301 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6361 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6421 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6481 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6541 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6601 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6661 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6721 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6781 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6841 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6901 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6961 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7021 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7081 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7141 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7201 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     7261 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7321 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     7381 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7441 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7501 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7561 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7621 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7681 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7741 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7801 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7861 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7921 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
     7981 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8041 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8101 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8161 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8221 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8281 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8341 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8401 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8461 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8521 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8581 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8641 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8701 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8761 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8821 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8881 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8941 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9001 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9061 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9121 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9181 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9241 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9301 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9361 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9421 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9481 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9541 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9601 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9661 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9721 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9781 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9841 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9901 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9961 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10021 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10081 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10141 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10201 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10261 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10321 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10381 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10441 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10501 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10561 ttgtaaaacg acggccagtg ccaagctg
//
