LOCUS       V045926                11733 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045926
VERSION     V045926
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5752
                     /label="Ddb1(NM_001410219)"
                     /note="Ddb1(NM_001410219)"
                     /gene="Ddb1"
     CDS             5759..5848
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5892..6103
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6116..6384
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6413..6422
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6443..7105
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7175..7228
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7229..7828
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7829..8416
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8490..8723
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8795..8916
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8935..9070
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9108..9124)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9132..9148
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9156..9186)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9201..9222
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9510..10098)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10269..11129)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11130..11234)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11708..11724
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgt cgtacaacta cgtcgtaacg gcgcagaagc ctactgcagt
     2341 gaacggctgt gtgaccggac actttacttc agcggaagat ctgaacctgt tgattgctaa
     2401 aaacacaaga ttagagatct atgtggtcac tgcggagggt cttcggccag tcaaagaggt
     2461 gggaatgtac gggaagattg cagtcatgga gctcttcagg cccaaggggg agagcaaaga
     2521 cctgctgttc atcttgactg ctaaatacaa tgcctgtatc ttggagtata agcagagtgg
     2581 cgaaagcatt gacatcatta caagagccca tggcaatgtc caggaccgca ttggtcgccc
     2641 ctcggagact ggcatcattg gcatcattga ccctgagtgc cgtatgattg gccttcgact
     2701 ctacgatggc cttttcaagg tcattccact agaccgagac aataaagagc tcaaggcctt
     2761 taacatccgc ctagaggaac tgcacgtcat tgatgtcaag ttcctgtatg gttgtcaagc
     2821 acctaccatt tgctttgtct accagcctct taaaggtgac tttggcagcc ccaagactga
     2881 tgatcctcag gggcggcatg tgaaaaccta tgaggtgtct cttcgagaga aggagttcaa
     2941 caagggccct tggaaacagg agaatgtaga agccgaagct tcaatggtga tagcagtccc
     3001 agagcctttt ggaggtgcca tcatcattgg acaggaatca atcacctatc ataacggtga
     3061 taaatacctg gcaattgctc ctcctatcat taagcaaagc actattgtgt gccataaccg
     3121 ggtggatcct aatggctcgc ggtaccttct gggagatatg gagggacggc ttttcatgct
     3181 gctcttagag aaggaggagc agatggatgg cactgtcacc ctcaaggacc ttcgagtgga
     3241 actcctcgga gagacctcca ttgctgagtg cctaacttat cttgataatg gtgttgtatt
     3301 tgtcgggtct cgcctaggtg actcccagct cgtaaagctc aatgtcgaca gcaacgaaca
     3361 aggctcctat gtagtggcca tggaaacctt taccaattta ggacccattg tggatatgtg
     3421 tgtggtggac ctggagaggc agggacaggg tcagctggtc acttgctctg gagctttcaa
     3481 ggaaggttcc ttgcggatca tccggaacgg cattggaatc catgagcatg ccagcattga
     3541 cttaccaggc atcaaaggtt tatggcctct gcgctctgac cccggtcggg agacggatga
     3601 cactttggtg ctctcttttg tgggccagac aagagttctc atgctaaacg gagaggaagt
     3661 ggaagagaca gaactgatgg gctttgtgga cgatcagcag actttctttt gtggcaacgt
     3721 ggctcatcag caacttatcc agatcacctc agcatctgtg aggttggtgt ctcaagagcc
     3781 caaagctttg gtcagcgagt ggaaagagcc tcagggcaag aacatcagtg tggcctcctg
     3841 taacagcagc caggtggttg ttgccgtggg aagggcacta tattaccttc agatccaccc
     3901 tcaggagctc cggcaaatca gccacacaga gatggaacat gaagtggcat gcttggatat
     3961 caccccatta ggggacagca atggcctctc cccactttgt gccattggac tttggacaga
     4021 catctcagct cgtatcctga agctcccatc ctttgaacta ctacacaagg agatgctggg
     4081 tggagagatc attcctcgat ccatcctgat gaccactttt gagagtagcc actacctcct
     4141 ttgtgccttg ggagatgggg ctcttttcta ctttgggctc aacatcgaga caggtttact
     4201 gagtgaccgt aaaaaggtga ctttgggcac ccagcccacg gtgttgagga cattccgttc
     4261 tctttctacc accaacgtct ttgcttgctc tgaccggccc actgtcatct atagcagcaa
     4321 tcacaagttg gtcttctcca atgtcaacct caaggaggtg aactatatgt gtcctctcaa
     4381 ctcagatggc tatcctgaca gtctggcatt ggccaataac agcactctca ccattggcac
     4441 catcgatgag atccagaagc tccatattcg cacagttccc ctctatgagt ctcccaggaa
     4501 gatctgctat caggaagtgt ctcagtgctt tggggtcctt tccagccgca ttgaagtcca
     4561 agatagcagt ggaggcacta ctgctttgag gcccagtgcc agcacccagg ccctgtccag
     4621 cagcgtcagc tccagcaaac ttttctccag cagtacggct cctcacgaga cctcctttgg
     4681 agaagaagtg gaggtgcaca acctcctcat cattgaccag cacacctttg aagtgcttca
     4741 cgcccatcag tttctacaaa acgagtatgc cctcagcctg gtctcctgca agttgggtaa
     4801 agaccccaac acttacttca ttgtgggcac agccatggta tatcctgaag aggcagagcc
     4861 caagcagggt cgtattgtgg tcttccagta ttcagatgga aaactacaga ctgtggctga
     4921 aaaggaagta aaaggagctg tgtactctat ggtggagttt aatgggaagc tgcttgccag
     4981 catcaatagc acggtgcggc tctatgagtg gaccacagaa aaggagcttc gcactgagtg
     5041 taatcactac aacaacatca tggccctgta cctgaagacc aagggcgact tcatcctggt
     5101 gggcgacctc atgcgctcag tgctgctgct tgcctacaag cctatggaag gaaactttga
     5161 agagattgcc cgagacttta atcccaactg gatgagtgca gtggagatct tggatgatga
     5221 caattttctg ggagctgaaa atgcctttaa cttgtttgtg tgtcagaagg acagtgctgc
     5281 caccactgat gaggagcggc agcacctaca ggaggttggt ctcttccacc tgggcgagtt
     5341 tgtcaatgtg ttctgccatg gctccctagt tatgcagaat ctgggcgagg cttctacccc
     5401 tacacagggc tcggtgctct ttggcacagt caacggcatg atagggctgg tgacctcgct
     5461 gtcagagagc tggtataacc tcctgttgga catgcagaac cgactcaata aggtcatcaa
     5521 aagcgtgggc aagattgagc actccttctg gagatccttt cacactgagc gaaagacaga
     5581 accagccaca ggcttcatcg atggtgatct gattgaaagt ttcctagata tcagccgccc
     5641 taagatgcag gaggttgtgg caaacttgca gtatgatgat ggcagtggta tgaagcggga
     5701 ggcaactgca gatgacctca tcaaagtcgt ggaggagcta actcggatcc atggatccga
     5761 acaaaaacta atatcagagg aagatttgga acagaaacta atcagtgaag aagatttaga
     5821 gcaaaagtta atttccgagg aggacttata gcggccgcga aggatctgcg atcgctccgg
     5881 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     5941 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     6001 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     6061 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc
     6121 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag
     6181 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag
     6241 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact
     6301 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt
     6361 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat
     6421 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct
     6481 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat
     6541 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca
     6601 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt
     6661 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg
     6721 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt
     6781 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg
     6841 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt
     6901 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat
     6961 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt
     7021 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc
     7081 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga
     7141 cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg
     7201 cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc
     7261 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     7321 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     7381 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     7441 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     7501 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     7561 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7621 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     7681 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     7741 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7801 gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag
     7861 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7921 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7981 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8041 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8101 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8161 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8221 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8281 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8341 gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct
     8401 ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     8461 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata
     8521 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8581 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8641 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8701 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8761 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8821 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8881 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8941 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9001 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9061 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt
     9121 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9181 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9241 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9301 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9361 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9421 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9481 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9541 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9601 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9661 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9721 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9781 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9841 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9901 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     9961 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10021 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10081 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10141 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10201 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10261 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10321 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10381 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10441 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10501 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10561 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10621 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10681 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10741 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10801 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10861 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10921 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10981 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11041 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11101 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11161 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11221 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    11281 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    11341 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    11401 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11461 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    11521 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    11581 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11641 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    11701 cgacgttgta aaacgacggc cagtgccaag ctg
//
