LOCUS       V047531                12934 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047531
VERSION     V047531
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6960
                     /label="DUOX2(NM_014080)"
                     /note="DUOX2(NM_014080)"
                     /gene="DUOX2"
     CDS             6967..7032
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7076..7287
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7300..7568
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7597..7606
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7627..8289
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8359..8412
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8413..9012
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9013..9601
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9675..9908
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9980..10101
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10141..10276
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10309..10325)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10333..10349
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10357..10387)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10402..10423
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10711..11299)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11470..12330)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12331..12435)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12909..12925
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tccgtgcaag accagaggca
     2341 ctgatgctcc tgggagctct tctgactgga tccctgggtc catcgggcaa tcaggacgca
     2401 ctctcactgc cctgggaagt gcagcgctat gacggctggt ttaacaacct gaggcaccac
     2461 gagcgtggtg ctgttggctg ccggttgcag cgccgcgtac cagccaatta cgccgacggt
     2521 gtgtatcagg ctctggagga gccgcagctg cccaacccgc gccggctcag caacgcagcc
     2581 acgcggggca tagccggcct gccgtcgctc cacaaccgca ccgtactggg ggtcttcttt
     2641 ggctaccatg ttctttccga cgtggtgagc gtggaaacgc ccggttgccc cgccgagttc
     2701 ctcaacatcc gcatcccacc tggagacccc gtgttcgacc ccgaccagcg cggggacgtg
     2761 gtgctgccct tccagaggag ccgctgggac cccgagaccg gacggagtcc cagcaacccc
     2821 cgggacctgg ccaaccaggt gacgggctgg ctggacggca gcgccatcta tggctcctcg
     2881 cactcctgga gcgacgcgct gcggagcttc tcggggggac agctggcgtc ggggcccgac
     2941 cccgctttcc cccgagactc gcagaacccc ctgctcatgt gggcggcgcc cgaccccgcc
     3001 accgggcaga acgggccccg ggggctgtac gccttcgggg cagagagagg gaaccgggaa
     3061 cccttcctgc aggcgctggg cctgctctgg ttccgctacc acaacctgtg ggcgcagagg
     3121 ctggcccgcc agcacccaga ctgggaggac gaggagctgt tccagcacgc acgcaagagg
     3181 gtcatcgcca cctaccagaa catcgctgtg tatgagtggc tgcccagctt cctgcagaaa
     3241 acactcccgg agtatacagg ataccgtcct ttcctagacc ccagcatctc cccggaattt
     3301 gtggtggcct ctgagcagtt cttctctacc atggtgcccc ctggtgtcta catgagaaat
     3361 gccagctgtc atttccggaa ggtcctgaac aagggttttc aaagctccca agctctcagg
     3421 gtctgcaaca actactggat tcgggagaac cccaatctga acagtaccca ggaggtgaat
     3481 gagctgctgc tgggaatggc ctcccagatt tcggagttgg aggacaacat agtggttgaa
     3541 gatctgaggg attactggcc tggccctggc aaattctccc gtacagacta tgtggccagc
     3601 agcatccaac gtggccgaga tatggggctg cccagctata gccaggccct gctggccttt
     3661 gggctggaca tcccaaggaa ctggagtgat ctcaacccta atgtggaccc ccaggtgctg
     3721 gaggccacag ctgccctgta caaccaggac ctatcccagc tagagctgct ccttgggggg
     3781 ctcctggaga gccatgggga ccctggaccc ctgttcagtg ccattgtcct cgaccagttt
     3841 gtacggctgc gggatggtga ccgctactgg tttgagaaca ccaggaatgg gctgttctcc
     3901 aagaaggaga ttgaagacat ccgaaatacc accctgcggg acgtgctggt cgctgttatc
     3961 aacattgacc ccagtgccct gcagcccaat gtctttgtct ggcataaagg tgcaccctgc
     4021 cctcaaccta agcagctcac aactgacggc ctgccccagt gtgcacccct gactgtgctt
     4081 gacttctttg aaggcagcag ccctggtttt gccatcacca tcattgctct ctgctgcctt
     4141 cccttagtga gtctgcttct ctctggagtg gtggcctatt tccggggccg agaacacaag
     4201 aagctacaaa agaaactcaa agagagcgtg aagaaggaag cagccaaaga tggagtgcca
     4261 gcgatggagt ggccaggccc caaggagagg agcagtccca tcatcatcca gctgctgtca
     4321 gacaggtgtc tgcaggtcct gaacaggcat ctcactgtgc tccgtgtggt ccagctgcag
     4381 cctctgcagc aggtcaacct catcctgtcc aacaaccgag gatgccgcac cctgctgctc
     4441 aagatcccta aggagtatga cctggtgctg ctgtttagtt ctgaagagga acggggcgcc
     4501 tttgtgcagc agctatggga cttctgcgtg cgctgggctc tgggcctcca tgtggctgag
     4561 atgagcgaga aggagctatt taggaaggct gtgacaaagc agcagcggga acgcatcctg
     4621 gagatcttct tcagacacct ttttgctcag gtgctggaca tcaaccaggc cgacgcaggg
     4681 accctgcccc tggactcctc ccagaaggtg cgggaggccc tgacctgcga gctgagcagg
     4741 gccgagtttg ccgagtccct gggcctcaag ccccaggaca tgtttgtgga gtccatgttc
     4801 tctctggctg acaaggatgg caatggctac ctgtccttcc gagagttcct ggacatcctg
     4861 gtggtcttca tgaaaggctc cccagaggat aagtcccgtc taatgtttac catgtatgac
     4921 ctggatgaga atggcttcct ctccaaggac gaattcttca ccatgatgcg atccttcatc
     4981 gagatctcca acaactgcct gtccaaggcc cagctggccg aggtggtgga gtctatgttc
     5041 cgggagtcgg gattccagga caaggaggag ctgacatggg aggattttca cttcatgctg
     5101 cgggaccatg acagcgagct ccgcttcacg cagctctgtg tcaaaggtgg aggtggaggt
     5161 ggaaatggta ttagagatat ctttaaacaa aacatcagct gtcgagtctc gttcatcact
     5221 cggacacctg gggagcgctc ccacccccag ggactggggc cccctgcccc agaagcccca
     5281 gagctgggag gccctggact gaagaagagg tttggcaaaa aggcagcagt gcccactccc
     5341 cggctgtaca cagaggcgct gcaagagaag atgcagcgag gcttcctagc ccaaaagctg
     5401 cagcagtaca agcgcttcgt ggagaactac cggaggcaca tcgtgtgtgt ggcaatcttc
     5461 tcggccatct gtgttggcgt gtttgcagat cgtgcttact actatggctt tgcctcgcca
     5521 ccctcggaca ttgcacagac caccctcgtg ggcatcatcc tgtcacgagg cacggcggcc
     5581 agcgtctcct tcatgttctc ttatatcttg ctcaccatgt gccgcaacct cataaccttc
     5641 ctgcgagaga ctttcctcaa ccgctatgtg ccttttgatg ccgcagtgga cttccaccgc
     5701 tggatcgcca tggctgctgt tgtcctggcc attttgcaca gtgctggcca cgcagtcaat
     5761 gtctacatct tctcagtcag cccactcagc ctgctggcct gcatattccc caacgtcttt
     5821 gtgaatgatg ggtccaagct tccccagaag ttctattggt ggttcttcca gaccgtccca
     5881 ggtatgacag gtgtgcttct gctcctggtc ctggccatca tgtatgtctt cgcctcccac
     5941 cacttccgcc gccgcagctt ccggggcttc tggctgaccc accacctcta catcctgctc
     6001 tatgccctgc tcatcatcca tggcagctat gctctgatcc agctgcccac tttccacatc
     6061 tacttcctgg tcccggcaat catctatgga ggtgacaagc tggtgagcct gagccggaag
     6121 aaggtggaga tcagcgtggt gaaggcggag ctgctgccct caggagtgac ctacctgcaa
     6181 ttccagaggc cccaaggctt tgagtacaag tcaggacagt gggtgcggat cgcctgcctg
     6241 gctctgggga ccaccgagta ccaccccttc acactgacct ccgcgcccca tgaggacaca
     6301 ctcagcctgc acatccgggc agtggggccc tggaccactc gcctcaggga gatctactca
     6361 tccccaaagg gcaatggctg tgctggatac ccaaagctgt accttgatgg accgtttgga
     6421 gagggccatc aggagtggca taaatttgag gtgtcagtgt tggtgggagg gggcattggg
     6481 gtcaccccct ttgcctccat cctcaaagac ctggtcttca agtcatcctt gggcagccaa
     6541 atgctgtgta agaagatcta cttcatctgg gtgacacgga cccagcgtca gtttgagtgg
     6601 ctggctgaca tcatccaaga ggtggaggag aacgaccacc aggacctggt gtctgtgcac
     6661 atttatgtca cccagctggc tgagaagttc gacctcagga ccaccatgct atacatctgc
     6721 gagcggcact tccagaaagt gctgaaccgg agtctgttca cgggcctgcg ctccatcacc
     6781 cactttggcc gtcccccctt cgagcccttc ttcaactccc tgcaggaggt ccacccacag
     6841 gtgcgcaaga tcggggtgtt cagctgcggc cctccaggaa tgaccaagaa tgtagagaag
     6901 gcctgtcagc tcgtcaacag gcaggaccga gcccacttca tgcaccacta tgagaacttc
     6961 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     7021 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     7081 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     7141 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     7201 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     7261 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     7321 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     7381 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     7441 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     7501 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     7561 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     7621 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     7681 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     7741 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     7801 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     7861 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     7921 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     7981 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     8041 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     8101 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     8161 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     8221 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     8281 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     8341 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     8401 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8461 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8521 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8581 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8641 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8701 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     8761 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     8821 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     8881 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     8941 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     9001 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     9061 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     9121 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     9181 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9241 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9301 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9361 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     9421 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9481 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9541 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9601 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9661 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     9721 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9781 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9841 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9901 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     9961 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
    10021 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10081 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
    10141 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10201 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10261 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
    10321 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10381 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10441 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10501 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10561 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10621 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10681 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10741 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10801 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10861 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10921 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10981 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11041 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11101 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    11161 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11221 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11281 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11401 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11461 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11521 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11581 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11641 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11701 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11761 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11821 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11881 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11941 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12001 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12061 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12121 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12181 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12241 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12301 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12361 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12421 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    12481 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    12541 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    12601 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    12661 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    12721 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    12781 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    12841 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    12901 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
