LOCUS       V042113                11644 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042113
VERSION     V042113
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5670
                     /label="DSG2(NM_001943)"
                     /note="DSG2(NM_001943)"
                     /gene="DSG2"
     CDS             5677..5742
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5786..5997
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6010..6278
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6307..6316
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6337..6999
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7069..7122
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7123..7722
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7723..8311
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8385..8618
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8690..8811
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8851..8986
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9019..9035)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9043..9059
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9067..9097)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9112..9133
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9421..10009)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10180..11040)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11041..11145)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11619..11635
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgcggagccc gggacgcgcg
     2341 tacgccctgc tgcttctcct gatctgcttt aacgttggaa gtggacttca cttacaggtc
     2401 ttaagcacaa gaaatgaaaa taagctgctt cctaaacatc ctcatttagt gcggcaaaag
     2461 cgcgcctgga tcaccgcccc cgtggctctt cgggagggag aggatctgtc caagaagaat
     2521 ccaattgcca agatacattc tgatcttgca gaagaaagag gactcaaaat tacttacaaa
     2581 tacactggaa aagggattac agagccacct tttggtatat ttgtctttaa caaagatact
     2641 ggagaactga atgttaccag cattcttgat cgagaagaaa caccattttt tctgctaaca
     2701 ggttacgctt tggatgcaag aggaaacaat gtagagaaac ccttagagct acgcattaag
     2761 gttcttgata tcaatgacaa cgaaccagtg ttcacacagg atgtctttgt tgggtctgtt
     2821 gaagagttga gtgcagcaca tactcttgtg atgaaaatca atgcaacaga tgcagatgag
     2881 cccaataccc tgaattcgaa aatttcctat agaatcgtat ctctggagcc tgcttatcct
     2941 ccagtgttct acctaaataa agatacagga gagatttata caaccagtgt taccttggac
     3001 agagaggaac acagcagcta cactttgaca gtagaagcaa gagatggcaa tggagaagtt
     3061 acagacaaac ctgtaaaaca agctcaagtt cagattcgta ttttggatgt caatgacaat
     3121 atacctgtag tagaaaataa agtgcttgaa gggatggttg aagaaaatca agtcaacgta
     3181 gaagttacgc gcataaaagt gttcgatgca gatgaaatag gttctgataa ttggctggca
     3241 aattttacat ttgcatcagg aaatgaagga ggttatttcc acatagaaac agatgctcaa
     3301 actaacgaag gaattgtgac ccttattaag gaagtagatt atgaagaaat gaagaatctt
     3361 gacttcagtg ttattgtcgc taataaagca gcttttcaca agtcgattag gagtaaatac
     3421 aagcctacac ccattcccat caaggtcaaa gtgaaaaatg tgaaagaagg cattcatttt
     3481 aaaagcagcg tcatctcaat ttatgttagc gagagcatgg atagatcaag caaaggccaa
     3541 ataattggaa attttcaagc ttttgatgag gacactggac taccagccca tgcaagatat
     3601 gtaaaattag aagatagaga taattggatc tctgtggatt ctgtcacatc tgaaattaaa
     3661 cttgcaaaac ttcctgattt tgaatctaga tatgttcaaa atggcacata cactgtaaag
     3721 attgtggcca tatcagaaga ttatcctaga aaaaccatca ctggcacagt ccttatcaat
     3781 gttgaagaca tcaacgacaa ctgtcccaca ctgatagagc ctgtgcagac aatctgtcac
     3841 gatgcagagt atgtgaatgt tactgcagag gacctggatg gacacccaaa cagtggccct
     3901 ttcagtttct ccgtcattga caaaccacct ggcatggcag aaaaatggaa aatagcacgc
     3961 caagaaagta ccagtgtgct gctgcaacaa agtgagaaaa agcttgggag aagtgaaatt
     4021 cagttcctga tttcagacaa tcagggtttt agttgtcctg aaaagcaggt ccttacactc
     4081 acagtttgtg agtgtctgca tggcagcggc tgcagggaag cacagcatga ctcctatgtg
     4141 ggcctgggac ccgcagcaat tgcgctcatg attttggcct ttctgctcct gctattggta
     4201 ccacttttac tgctgatgtg ccattgcgga aagggcgcca aaggctttac ccccatacct
     4261 ggcaccatag agatgctgca tccttggaat aatgaaggag caccacctga agacaaggtg
     4321 gtgccatcat ttctgccagt ggatcaaggg ggcagtctag taggaagaaa tggagtagga
     4381 ggtatggcca aggaagccac gatgaaagga agtagctctg cttccattgt caaagggcaa
     4441 catgagatgt ccgagatgga tggaaggtgg gaagaacaca gaagcctgct ttctggtaga
     4501 gctacccagt ttacaggggc cacaggcgct atcatgacca ctgaaaccac gaagaccgca
     4561 agggccacag gggcttccag agacatggcc ggagctcagg cagctgctgt tgcactgaac
     4621 gaagaattct taagaaatta tttcactgat aaagcggcct cttacactga ggaagatgaa
     4681 aatcacacag ccaaagattg ccttctggtt tattctcagg aagaaactga atcgctgaat
     4741 gcttctattg gttgttgcag ttttattgaa ggagagctag atgaccgctt cttagatgat
     4801 ttgggactta aattcaagac actagctgaa gtttgcctgg gtcaaaaaat agatataaat
     4861 aaggaaattg agcagagaca aaaacctgcc acagaaacaa gtatgaacac agcttcacat
     4921 tcactctgtg agcaaactat ggttaattca gagaatacct actcctctgg cagtagcttc
     4981 ccagttccaa aatctttgca agaagccaat gcagagaaag taactcagga aatagtcact
     5041 gaaagatctg tgtcttctag gcaggcgcaa aaggtagcta cacctcttcc tgacccaatg
     5101 gcttctagaa atgtgatagc aacagaaact tcctatgtca cagggtccac tatgccacca
     5161 accactgtga tcctgggtcc tagccagcca cagagcctta ttgtgacaga gagggtgtat
     5221 gctccagctt ctaccttggt agatcagcct tatgctaatg aaggtacagt tgtggtcact
     5281 gaaagagtaa tacagcctca tgggggtgga tcgaatcctc tggaaggcac tcagcatctt
     5341 caagatgtac cttacgtcat ggtgagggaa agagagagct tccttgcccc cagctcaggt
     5401 gtgcagccta ctctggccat gcctaatata gcagtaggac agaatgtgac agtgacagaa
     5461 agagttctag cacctgcttc cactctgcaa tccagttacc agattcccac tgaaaattct
     5521 atgacggcta ggaacaccac ggtgtctgga gctggagtcc ctggccctct gccagatttt
     5581 ggtttagagg aatctggtca ttctaattct accataacca catcttccac cagagttacc
     5641 aagcatagca ctgtacagca ttcttactcc ggatccgact acaaagacca tgacggtgat
     5701 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     5761 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5821 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     5881 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5941 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     6001 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     6061 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     6121 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     6181 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     6241 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     6301 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     6361 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     6421 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     6481 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     6541 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     6601 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     6661 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     6721 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     6781 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     6841 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     6901 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     6961 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     7021 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     7081 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     7141 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     7201 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     7261 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     7321 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     7381 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     7441 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7501 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7561 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     7621 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7681 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     7741 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7801 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7861 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7921 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7981 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8041 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8101 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8161 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8221 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8281 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     8341 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8401 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8461 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8521 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8581 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8641 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     8701 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8761 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8821 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8881 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8941 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     9001 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9061 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9121 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9181 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9241 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9841 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11161 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    11221 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    11281 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    11341 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    11401 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11461 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    11521 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    11581 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    11641 gctg
//
